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Pathog Glob Health. 2021 Jul;115(5):307-314. doi: 10.1080/20477724.2021.1896265. Epub 2021 Mar 09.

Diagnosing human cutaneous leishmaniasis using fluorescence .

Pathogens and global health

Thilini Jayasena Kaluarachchi, Rajitha Wickremasinghe, Manjula Weerasekera, Surangi Yasawardene, Andrew J McBain, Bandujith Yapa, Hiromel De Silva, Chandranie Menike, Subodha Jayathilake, Anuradha Munasinghe, Renu Wickremasinghe, Shalindra Ranasinghe

Affiliations

  1. Department of Parasitology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  2. Department of Public Health, Faculty of Medicine, University of Kelaniya, Sri Lanka.
  3. Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  4. Department of Anatomy, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  5. Division of Pharmacy & Optometry, School of Health Sciences, Faculty of Biology, Medicine and Health, the University of Manchester, UK.
  6. Dermatology Unit, District General Hospital, Matara, Sri Lanka.
  7. Dermatology Unit, Base Hospital, Tangalle, Sri Lanka.
  8. Department of Pathology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
  9. Department of Transport and Logistics Management, University of Moratuwa, Katubedda, Moratuwa, Sri Lanka.

PMID: 33686909 PMCID: PMC8547812 DOI: 10.1080/20477724.2021.1896265

Abstract

Cutaneous leishmaniasis (CL) is endemic in Sri Lanka. Giemsa-stained slit-skin-smears (SSS-Giemsa) and histology are routinely used in diagnosis with a sensitivity of 40-70%. PCR currently has limited accessibility. Therefore, we assessed the sensitivity and specificity of a previously described fluorescence

Keywords: Cutaneous leishmaniasis; Sri Lanka; fluorescence in situ hybridization

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