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López-Varea A, Vega-Cuesta P, Ruiz-Gómez A, et al. Genome-wide phenotypic RNAi screen in the Drosophila wing: phenotypic description of functional classes. G3 (Bethesda). 2021;11(12)doi: 10.1093/g3journal/jkab349.
López-Varea, A., Vega-Cuesta, P., Ruiz-Gómez, A., Ostalé, C. M., Molnar, C., Hevia, C. F., Martín, M., Organista, M. F., de Celis, J., Culí, J., Esteban, N., & de Celis, J. F. (2021). Genome-wide phenotypic RNAi screen in the Drosophila wing: phenotypic description of functional classes. G3 (Bethesda, Md.), 11(12), . https://doi.org/10.1093/g3journal/jkab349
López-Varea, Ana, et al. "Genome-wide phenotypic RNAi screen in the Drosophila wing: phenotypic description of functional classes." G3 (Bethesda, Md.) vol. 11,12 (2021). doi: https://doi.org/10.1093/g3journal/jkab349
López-Varea A, Vega-Cuesta P, Ruiz-Gómez A, Ostalé CM, Molnar C, Hevia CF, Martín M, Organista MF, de Celis J, Culí J, Esteban N, de Celis JF. Genome-wide phenotypic RNAi screen in the Drosophila wing: phenotypic description of functional classes. G3 (Bethesda). 2021 Dec 08;11(12). doi: 10.1093/g3journal/jkab349. PMID: 34599810; PMCID: PMC8664486.
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G3 (Bethesda). 2021 Dec 08;11(12). doi: 10.1093/g3journal/jkab349.

Genome-wide phenotypic RNAi screen in the Drosophila wing: phenotypic description of functional classes.

G3 (Bethesda, Md.)

Ana López-Varea, Patricia Vega-Cuesta, Ana Ruiz-Gómez, Cristina M Ostalé, Cristina Molnar, Covadonga F Hevia, Mercedes Martín, Maria F Organista, Jesus de Celis, Joaquín Culí, Nuria Esteban, Jose F de Celis

Affiliations

  1. Centro de Biología Molecular "Severo Ochoa," CSIC and Universidad Autónoma de Madrid, Madrid 28049, Spain.
  2. IRB Barcelona, Barcelona 08028, Spain.

PMID: 34599810 PMCID: PMC8664486 DOI: 10.1093/g3journal/jkab349

Abstract

The Drosophila genome contains approximately 14,000 protein-coding genes encoding all the necessary information to sustain cellular physiology, tissue organization, organism development, and behavior. In this manuscript, we describe in some detail the phenotypes in the adult fly wing generated after knockdown of approximately 80% of Drosophila genes. We combined this phenotypic description with a comprehensive molecular classification of the Drosophila proteins into classes that summarize the main expected or known biochemical/functional aspect of each protein. This information, combined with mRNA expression levels and in situ expression patterns, provides a simplified atlas of the Drosophila genome, from housekeeping proteins to the components of the signaling pathways directing wing development, that might help to further understand the contribution of each gene group to wing formation.

© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

Keywords: RNAi; phenotype; screen; wing

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