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Panda S, Jozwiak A, Sonawane PD, et al. Steroidal alkaloids defence metabolism and plant growth are modulated by the joint action of gibberellin and jasmonate signalling. New Phytol. 2021;233(3):1220-1237doi: 10.1111/nph.17845.
Panda, S., Jozwiak, A., Sonawane, P. D., Szymanski, J., Kazachkova, Y., Vainer, A., Vasuki Kilambi, H., Almekias-Siegl, E., Dikaya, V., Bocobza, S., Shohat, H., Meir, S., Wizler, G., Giri, A. P., Schuurink, R., Weiss, D., Yasuor, H., Kamble, A., & Aharoni, A. (2022). Steroidal alkaloids defence metabolism and plant growth are modulated by the joint action of gibberellin and jasmonate signalling. The New phytologist, 233(3), 1220-1237. https://doi.org/10.1111/nph.17845
Panda, Sayantan, et al. "Steroidal alkaloids defence metabolism and plant growth are modulated by the joint action of gibberellin and jasmonate signalling." The New phytologist vol. 233,3 (2022): 1220-1237. doi: https://doi.org/10.1111/nph.17845
Panda S, Jozwiak A, Sonawane PD, Szymanski J, Kazachkova Y, Vainer A, Vasuki Kilambi H, Almekias-Siegl E, Dikaya V, Bocobza S, Shohat H, Meir S, Wizler G, Giri AP, Schuurink R, Weiss D, Yasuor H, Kamble A, Aharoni A. Steroidal alkaloids defence metabolism and plant growth are modulated by the joint action of gibberellin and jasmonate signalling. New Phytol. 2022 Feb;233(3):1220-1237. doi: 10.1111/nph.17845. Epub 2021 Dec 03. PMID: 34758118.
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New Phytol. 2022 Feb;233(3):1220-1237. doi: 10.1111/nph.17845. Epub 2021 Dec 03.

Steroidal alkaloids defence metabolism and plant growth are modulated by the joint action of gibberellin and jasmonate signalling.

The New phytologist

Sayantan Panda, Adam Jozwiak, Prashant D Sonawane, Jedrzej Szymanski, Yana Kazachkova, Andrii Vainer, Himabindu Vasuki Kilambi, Efrat Almekias-Siegl, Varvara Dikaya, Samuel Bocobza, Hagai Shohat, Sagit Meir, Guy Wizler, Ashok P Giri, Robert Schuurink, David Weiss, Hagai Yasuor, Avinash Kamble, Asaph Aharoni

Affiliations

  1. Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 7610001, Israel.
  2. Gilat Research Center, Agricultural Research Organization (ARO), Rural delivery Negev, 85280, Israel.
  3. Department of Botany, Savitribai Phule Pune University, Ganeshkhind, Pune, 411007, India.
  4. Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
  5. Department of Biology I, Ludwig-Maximilians-University of Munich, Munich, Germany.
  6. Department of Vegetable Research, ARO-Volcani Center, Bet Dagan, 50250, Israel.
  7. Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel.
  8. Plant Molecular Biology Unit, Division of Biochemical Sciences, Council of Scientific and Industrial Research-National Chemical Laboratory, Pune, 411008, India.
  9. Green Life Sciences Research Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, the Netherlands.

PMID: 34758118 DOI: 10.1111/nph.17845

Abstract

Steroidal glycoalkaloids (SGAs) are protective metabolites constitutively produced by Solanaceae species. Genes and enzymes generating the vast structural diversity of SGAs have been largely identified. Yet, mechanisms of hormone pathways coordinating defence (jasmonate; JA) and growth (gibberellin; GA) controlling SGAs metabolism remain unclear. We used tomato to decipher the hormonal regulation of SGAs metabolism during growth vs defence tradeoff. This was performed by genetic and biochemical characterisation of different JA and GA pathways components, coupled with in vitro experiments to elucidate the crosstalk between these hormone pathways mediating SGAs metabolism. We discovered that reduced active JA results in decreased SGA production, while low levels of GA or its receptor led to elevated SGA accumulation. We showed that MYC1 and MYC2 transcription factors mediate the JA/GA crosstalk by transcriptional activation of SGA biosynthesis and GA catabolism genes. Furthermore, MYC1 and MYC2 transcriptionally regulate the GA signalling suppressor DELLA that by itself interferes in JA-mediated SGA control by modulating MYC activity through protein-protein interaction. Chemical and fungal pathogen treatments reinforced the concept of JA/GA crosstalk during SGA metabolism. These findings revealed the mechanism of JA/GA interplay in SGA biosynthesis to balance the cost of chemical defence with growth.

© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.

Keywords: defence; gibberellin (GA); growth; jasmonate (JA); steroidal glycoalkaloids (SGAs); tomato; α-tomatine

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