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Sarabhai T, Koliaki C, Mastrototaro L, et al. Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle. Diabetologia. 2021;65(2):301-314doi: 10.1007/s00125-021-05596-z.
Sarabhai, T., Koliaki, C., Mastrototaro, L., Kahl, S., Pesta, D., Apostolopoulou, M., Wolkersdorfer, M., Bönner, A. C., Bobrov, P., Markgraf, D. F., Herder, C., & Roden, M. (2022). Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle. Diabetologia, 65(2), 301-314. https://doi.org/10.1007/s00125-021-05596-z
Sarabhai, Theresia, et al. "Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle." Diabetologia vol. 65,2 (2022): 301-314. doi: https://doi.org/10.1007/s00125-021-05596-z
Sarabhai T, Koliaki C, Mastrototaro L, Kahl S, Pesta D, Apostolopoulou M, Wolkersdorfer M, Bönner AC, Bobrov P, Markgraf DF, Herder C, Roden M. Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle. Diabetologia. 2022 Feb;65(2):301-314. doi: 10.1007/s00125-021-05596-z. Epub 2021 Oct 26. PMID: 34704121.
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Diabetologia. 2022 Feb;65(2):301-314. doi: 10.1007/s00125-021-05596-z. Epub 2021 Oct 26.

Dietary palmitate and oleate differently modulate insulin sensitivity in human skeletal muscle.

Diabetologia

Theresia Sarabhai, Chrysi Koliaki, Lucia Mastrototaro, Sabine Kahl, Dominik Pesta, Maria Apostolopoulou, Martin Wolkersdorfer, Anna C Bönner, Pavel Bobrov, Daniel F Markgraf, Christian Herder, Michael Roden

Affiliations

  1. Department of Endocrinology and Diabetology, Medical Faculty and University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany.
  2. Institute for Clinical Diabetology, German Diabetes Center, Leibniz Institute for Diabetes Research at Heinrich-Heine-University, Düsseldorf, Germany.
  3. German Center for Diabetes Research, Partner Düsseldorf, Neuherberg, Germany.
  4. Landesapotheke Salzburg, Department of Production, Hospital Pharmacy, Salzburg, Austria.
  5. Institute for Biometrics and Epidemiology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich-Heine-University, Düsseldorf, Germany.
  6. Department of Endocrinology and Diabetology, Medical Faculty and University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany. [email protected].
  7. Institute for Clinical Diabetology, German Diabetes Center, Leibniz Institute for Diabetes Research at Heinrich-Heine-University, Düsseldorf, Germany. [email protected].
  8. German Center for Diabetes Research, Partner Düsseldorf, Neuherberg, Germany. [email protected].

PMID: 34704121 DOI: 10.1007/s00125-021-05596-z

Abstract

AIMS/HYPOTHESIS: Energy-dense nutrition generally induces insulin resistance, but dietary composition may differently affect glucose metabolism. This study investigated initial effects of monounsaturated vs saturated lipid meals on basal and insulin-stimulated myocellular glucose metabolism and insulin signalling.

METHODS: In a randomised crossover study, 16 lean metabolically healthy volunteers received single meals containing safflower oil (SAF), palm oil (PAL) or vehicle (VCL). Whole-body glucose metabolism was assessed from glucose disposal (R

RESULTS: SAF and PAL raised plasma oleate, but only PAL significantly increased plasma palmitate concentrations. SAF and PAL increased myocellular diacylglycerol and activated protein kinase C (PKC) isoform θ (p < 0.05) but only PAL activated PKCɛ. Moreover, PAL led to increased myocellular ceramides along with stimulated PKCζ translocation (p < 0.05 vs SAF). During clamp, SAF and PAL both decreased insulin-stimulated R

CONCLUSIONS/INTERPRETATION: Lipid-induced myocellular insulin resistance is likely more pronounced with palmitate than with oleate and is associated with PKC isoforms activation and inhibitory insulin signalling.

TRIAL REGISTRATION: ClinicalTrials.gov .NCT01736202.

FUNDING: German Federal Ministry of Health, Ministry of Culture and Science of the State North Rhine-Westphalia, German Federal Ministry of Education and Research, European Regional Development Fund, German Research Foundation, German Center for Diabetes Research.

© 2021. The Author(s).

Keywords: Glucose metabolism; Insulin signalling; Lipotoxicity; Monounsaturated fatty acids; Saturated fat; Skeletal muscle

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