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Filik K, Szermer-Olearnik B, Niedziółka-Jönson J, et al. φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3. AMB Express. 2022;12(1):1doi: 10.1186/s13568-021-01341-2.
Filik, K., Szermer-Olearnik, B., Niedziółka-Jönson, J., Roźniecka, E., Ciekot, J., Pyra, A., Matyjaszczyk, I., Skurnik, M., & Brzozowska, E. (2022). φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3. AMB Express, 12(1), 1. https://doi.org/10.1186/s13568-021-01341-2
Filik, Karolina, et al. "φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3." AMB Express vol. 12,1 (2022): 1. doi: https://doi.org/10.1186/s13568-021-01341-2
Filik K, Szermer-Olearnik B, Niedziółka-Jönson J, Roźniecka E, Ciekot J, Pyra A, Matyjaszczyk I, Skurnik M, Brzozowska E. φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3. AMB Express. 2022 Jan 06;12(1):1. doi: 10.1186/s13568-021-01341-2. PMID: 34989907.
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AMB Express. 2022 Jan 06;12(1):1. doi: 10.1186/s13568-021-01341-2.

φYeO3-12 phage tail fiber Gp17 as a promising high specific tool for recognition of Yersinia enterocolitica pathogenic serotype O:3.

AMB Express

Karolina Filik, Bożena Szermer-Olearnik, Joanna Niedziółka-Jönson, Ewa Roźniecka, Jarosław Ciekot, Anna Pyra, Irwin Matyjaszczyk, Mikael Skurnik, Ewa Brzozowska

Affiliations

  1. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl St, 53114, Wroclaw, Poland.
  2. Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44, 5201-224, Warsaw, Poland.
  3. Faculty of Chemistry, University of Wroclaw, 14 F. Joliot-Curie St, 50383, Wroclaw, Poland.
  4. Department of Mycology and Genetics, Institute of Genetics and Microbiology, University of Wroc?aw, 51-148, Wroclaw, Poland.
  5. Department of Bacteriology and Immunology, Faculty of Medicine, Human Microbiome Research Program, University of Helsinki, Helsinki, Finland.
  6. Division of Clinical Microbiology, Helsinki University Hospital, HUSLAB, Helsinki, Finland.
  7. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 12 R. Weigl St, 53114, Wroclaw, Poland. [email protected].

PMID: 34989907 DOI: 10.1186/s13568-021-01341-2

Abstract

Yersiniosis is an infectious zoonotic disease caused by two enteropathogenic species of Gram-negative genus Yersinia: Yersinia enterocolitica and Yersinia pseudotuberculosis. Pigs and other wild and domestic animals are reservoirs for these bacteria. Infection is usually spread to humans by ingestion of contaminated food. Yersiniosis is considered a rare disease, but recent studies indicate that it is overlooked in the diagnostic process therefore the infections with this bacterium are not often identified. Reliable diagnosis of Yersiniosis by culturing is difficult due to the slow growth of the bacteria easily overgrown by other more rapidly growing microbes unless selec-tive growth media is used. Phage adhesins recognizing bacteria in a specific manner can be an excellent diagnostic tool, es-pecially in the diagnosis of pathogens difficult for culturing. In this study, it was shown that Gp17, the tail fiber protein (TFP) of phage φYeO3-12, specifically recognizes only the pathogenic Yersinia enterocolitica serotype O:3 (YeO:3) bacteria. The ELISA test used in this work confirmed the specific interaction of this protein with YeO:3 and demonstrated a promising tool for developing the pathogen recognition method based on phage adhesins.

© 2022. The Author(s).

Keywords: Diagnostic; ELISA; Phage; Phage adhesins; Tail fiber protein; Yersinia enterocolitica; Yersiniosis

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