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Dos Santos DR, Fiais GA, de Oliveira Passos A, et al. Effects of orchiectomy and testosterone replacement therapy on redox balance and salivary gland function in Wistar rats. J Steroid Biochem Mol Biol. 2021;218:106048doi: 10.1016/j.jsbmb.2021.106048.
Dos Santos, D. R., Fiais, G. A., de Oliveira Passos, A., Dos Santos, L. F. G., Kayahara, G. M., Crivelini, M. M., Matsushita, D. H., Antoniali, C., Nakamune, A. C. M. S., Dornelles, R. C. M., & Chaves-Neto, A. H. (2021). Effects of orchiectomy and testosterone replacement therapy on redox balance and salivary gland function in Wistar rats. The Journal of steroid biochemistry and molecular biology, 218106048. https://doi.org/10.1016/j.jsbmb.2021.106048
Dos Santos, Damáris Raissa, et al. "Effects of orchiectomy and testosterone replacement therapy on redox balance and salivary gland function in Wistar rats." The Journal of steroid biochemistry and molecular biology vol. 218 (2021): 106048. doi: https://doi.org/10.1016/j.jsbmb.2021.106048
Dos Santos DR, Fiais GA, de Oliveira Passos A, Dos Santos LFG, Kayahara GM, Crivelini MM, Matsushita DH, Antoniali C, Nakamune ACMS, Dornelles RCM, Chaves-Neto AH. Effects of orchiectomy and testosterone replacement therapy on redox balance and salivary gland function in Wistar rats. J Steroid Biochem Mol Biol. 2021 Dec 29;218:106048. doi: 10.1016/j.jsbmb.2021.106048. Epub 2021 Dec 29. PMID: 34973376.
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J Steroid Biochem Mol Biol. 2021 Dec 29;218:106048. doi: 10.1016/j.jsbmb.2021.106048. Epub 2021 Dec 29.

Effects of orchiectomy and testosterone replacement therapy on redox balance and salivary gland function in Wistar rats.

The Journal of steroid biochemistry and molecular biology

Damáris Raissa Dos Santos, Gabriela Alice Fiais, Arthur de Oliveira Passos, Luis Fernando Gadioli Dos Santos, Giseli Mitsuy Kayahara, Marcelo Macedo Crivelini, Doris Hissako Matsushita, Cristina Antoniali, Ana Cláudia de Melo Stevanato Nakamune, Rita Cássia Menegati Dornelles, Antonio Hernandes Chaves-Neto

Affiliations

  1. Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas - SBFis, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
  2. Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
  3. Department of Diagnosis and Surgery, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
  4. Department of Basic Sciences, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil; Programa de Pós-Graduação Multicêntrico em Ciências Fisiológicas - SBFis, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil. Electronic address: [email protected].

PMID: 34973376 DOI: 10.1016/j.jsbmb.2021.106048

Abstract

The objective of this study was to investigate the effects of orchiectomy (ORX) and testosterone replacement therapy (TRT) on redox balance and function of salivary glands. Forty-five young adult male Wistar rats (3 months old) were either castrated bilaterally or underwent fictitious surgery (SHAM) and were subsequently distributed into 3 groups: SHAM, ORX, and TRT (castrated rats that received an intramuscular injection of testosterone cypionate 10 mg/kg/weekly). All treatments started 4 weeks after castration (4 months old) and lasted 4 weeks (5 months old). At the end of treatment, pilocarpine-induced salivary secretion was collected to analyze salivary flow rate and biochemistry composition, and the parotid (PG) and submandibular (SMG) glands were sampled for redox balance markers and histomorphometric analyses. ORX increased salivary flow rate, calcium, phosphate, and chloride, and decreased total protein and amylase, while not changing the salivary buffer capacity, pH, sodium, and potassium compared to SHAM. TRT restored all salivary parameters to SHAM values. ORX increased oxidative lipid and protein damage, total antioxidant capacity, and uric acid in both salivary glands compared to SHAM. Superoxide dismutase, catalase, and glutathione peroxidase activities were greater only in the SMG of the ORX group in relation to SHAM. ORX decreased duct and acini area, while increasing connective tissue in the PG. On the other hand, ORX reduced duct area and increased acini area in the SMG compared to SHAM. TRT restored the redox balance and histomorphometric parameters to close to SHAM values in both salivary glands. Orchiectomy-induced salivary gland dysfunction was characterized by an increase in the salivary flow rate and changes in the secretion of total protein, amylase, and electrolytes, which are key factors, considered important for maintaining oral health status. To sum up, orchiectomy impaired the redox balance of the salivary glands. Our results also showed that TRT reversed the oxidative damage, morphological alterations, and salivary gland dysfunction induced by orchiectomy. Therefore, these results suggest an important action of testosterone on the redox balance and secretory ability of salivary glands.

Copyright © 2021 Elsevier Ltd. All rights reserved.

Keywords: Orchiectomy; Oxidative stress; Saliva; Salivary glands; Testosterone cypionate

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