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Kato K, Udagawa R, Hayashi Y, et al. SF3B14 is involved in the centrosome regulation through splicing of TUBGCP6 pre-mRNA. Biochem Biophys Res Commun. 2021;588:133-139doi: 10.1016/j.bbrc.2021.12.059.
Kato, K., Udagawa, R., Hayashi, Y., Maki, M., Yanagida, M., Higashiura, S., Yagishita, R., Shimamoto, H., & Kimura, K. (2022). SF3B14 is involved in the centrosome regulation through splicing of TUBGCP6 pre-mRNA. Biochemical and biophysical research communications, 588133-139. https://doi.org/10.1016/j.bbrc.2021.12.059
Kato, Kazashi, et al. "SF3B14 is involved in the centrosome regulation through splicing of TUBGCP6 pre-mRNA." Biochemical and biophysical research communications vol. 588 (2022): 133-139. doi: https://doi.org/10.1016/j.bbrc.2021.12.059
Kato K, Udagawa R, Hayashi Y, Maki M, Yanagida M, Higashiura S, Yagishita R, Shimamoto H, Kimura K. SF3B14 is involved in the centrosome regulation through splicing of TUBGCP6 pre-mRNA. Biochem Biophys Res Commun. 2022 Jan 15;588:133-139. doi: 10.1016/j.bbrc.2021.12.059. Epub 2021 Dec 18. PMID: 34954520.
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Biochem Biophys Res Commun. 2022 Jan 15;588:133-139. doi: 10.1016/j.bbrc.2021.12.059. Epub 2021 Dec 18.

SF3B14 is involved in the centrosome regulation through splicing of TUBGCP6 pre-mRNA.

Biochemical and biophysical research communications

Kazashi Kato, Rina Udagawa, Yuki Hayashi, Masayoshi Maki, Makiko Yanagida, Sae Higashiura, Reina Yagishita, Haruka Shimamoto, Keiji Kimura

Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki, 305-8577, Japan.
  2. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki, 305-8577, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki, 305-8577, Japan.
  3. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki, 305-8577, Japan; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki, 305-8577, Japan. Electronic address: [email protected].

PMID: 34954520 DOI: 10.1016/j.bbrc.2021.12.059

Abstract

Splicing precursor messenger RNA (pre-mRNA) is a critical step to produce physiologically functional protein. Splicing failure not only gives rise to dysfunctional proteins but also generates abnormal protein function, which causes several diseases. Several pre-mRNA splicing factors are reported to regulate mitosis directly at mitotic structures and/or indirectly through controlling the pre-mRNA splicing for mitotic proteins. In this study, we described the mitotic functions of SF3B14, a component of the spliceosomal U2 small nuclear ribonucleoprotein (snRNP), which we identified as a candidate involved in mitosis based on the large-scale RNA interference (RNAi) screen of the nucleolar proteome database. We observed that SF3B14 depletion caused prolonged mitosis and several mitotic defects, such as monopolar spindle and chromosome misalignment during metaphase. Although SF3B14 was found in the nucleolar proteome database, our immunofluorescent stainings demonstrated that SF3B14 was predominantly localized in the nucleoplasm and excluded from the nucleolus during interphase. In addition, SF3B14 did not colocalize with specific mitotic structures during mitosis, which is not in line with its direct mitotic function. Notably, we found that the SF3B14 depletion reduced protein levels of TUBGCP6, required for centrosome regulation, and increased the unspliced/spliced ratio of its mRNA. Taken together, we propose that the pre-mRNA of TUBGCP6 is one of the targets for SF3B14 splicing through which SF3B14 controls mitotic chromosome behavior.

Copyright © 2021 Elsevier Inc. All rights reserved.

Keywords: Centrosomes; Mitosis; Splicing factors; TUBGCP6

Conflict of interest statement

Declaration of competing interest The authors declare no conflict of the interests.

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