Curr Pharm Biotechnol. 2021 May 31; doi: 10.2174/1389201022666210531120935. Epub 2021 May 31.
Revealing the promoting effect of betaine on vitamin B12 biosynthetic pathway of Pseudomonas denitrificans by using a proteomics analysis.
Current pharmaceutical biotechnology
Kun-Tai Li, Yong Yang, Xin Cheng
Affiliations
Affiliations
- College of Food Science and Technology, Guangdong Provincial Key Labotatory of Aquatic Product Processing and Safety, Guangdong Ocean University, Zhanjiang 524088, China.
- University of Shanghai for Science and Technology, Shanghai Engineering Research Center of Food Microbiology, Shanghai 200093, China.
- Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Jiangxi Agricultural University, Nanchang 330045, China.
PMID: 34061014
DOI: 10.2174/1389201022666210531120935
Abstract
BACKGROUND: Our previous comparative metabolomics research revealed that betaine (N,N,N-trimethylglycine, a typically essential methyl-group donor for vitamin B12 biosynthesis) had a powerful promoting effect on the generation of vitamin B12 precursors and intermediates in vitamin B12-producing Pseudomonas denitrificans. However, the integral effect of betaine on the vitamin B12 biosynthetic pathway is still unclear.
OBJECTIVE: Considering the vitamin B12 biosynthetic pathway of P. denitrificans as a whole, this work aimed to reveal the biological function of betaine on the vitamin B12 biosynthetic pathway in P. denitrificans, which would sharpen and expand the understanding of betaine as the methyl-group donor for vitamin B12 biosynthesis.
MATERIALS AND METHODS: By using a proteomics method based on the iTRAQ technique, the present study compared and analyzed the differential expression of proteins involved in vitamin B12 biosynthetic pathway under 10 g/L betaine addition to P. denitrificans fermentation medium.
RESULTS: The results showed that betaine could significantly up-regulate the expression of proteins related to the vitamin B12 biosynthetic pathway, which was mainly reflected in the following three aspects: 1) the δ-aminolevulinic acid (ALA) synthase and porphobilinogen synthase that were responsible for the formation of the committed precursors for tetrapyrrole-derived macrocycle in vitamin B12 molecule; 2) the C-methylation-related enzymes (such as precorrin-4 C(11)-methyltransferase, Precorrin-2 C(20)-methyltransferase, Precorrin-8X methylmutase, and Precorrin-6Y C5,15-methyltransferase) and methionine synthase that were crucial to the C-methylation reactions for vitamin B12 biosynthesis; 3) the late-stage key enzymes (Cobaltochelatase, and Cob(I)yrinic acid a,c-diamide adenosyltransferase) that were related to cobalt chelation of vitamin B12 molecule.
CONCLUSIONS: The present study clearly demonstrated that betaine could significantly promote the expression of the integral enzymes involved in the vitamin B12 biosynthetic pathway of P. denitrificans, thus promoting vitamin B12 biosynthesis.
Copyright© Bentham Science Publishers; For any queries, please email at [email protected].
Keywords: Pseudomonas denitrificans; betaine; differential expression of proteins; promoting effect; proteomics analysis
; vitamin B12 biosynthetic pathway
Publication Types
Grant support
