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Massel K, Lam Y, Hintzsche J, et al. Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals. Plant Cell Rep. 2021;doi: 10.1007/s00299-021-02816-z.
Massel, K., Lam, Y., Hintzsche, J., Lester, N., Botella, J. R., & Godwin, I. D. (2021). Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals. Plant cell reports, . https://doi.org/10.1007/s00299-021-02816-z
Massel, Karen, et al. "Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals." Plant cell reports vol. (2021). doi: https://doi.org/10.1007/s00299-021-02816-z
Massel K, Lam Y, Hintzsche J, Lester N, Botella JR, Godwin ID. Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals. Plant Cell Rep. 2021 Dec 02; doi: 10.1007/s00299-021-02816-z. Epub 2021 Dec 02. PMID: 34854968.
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Plant Cell Rep. 2021 Dec 02; doi: 10.1007/s00299-021-02816-z. Epub 2021 Dec 02.

Endogenous U6 promoters improve CRISPR/Cas9 editing efficiencies in Sorghum bicolor and show potential for applications in other cereals.

Plant cell reports

Karen Massel, Yasmine Lam, Jessica Hintzsche, Nicholas Lester, Jose R Botella, Ian D Godwin

Affiliations

  1. Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, 4072, Australia. [email protected].
  2. Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD, 4072, Australia.
  3. School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia.

PMID: 34854968 DOI: 10.1007/s00299-021-02816-z

Abstract

KEY MESSAGE: Endogenous U6 promoters increase CRISPR/Cas9 editing efficiency in sorghum and may be useful for gene editing applications in other cereals.

© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords: Cereals; Gene editing; Promoters; Sorghum

References

  1. Jiang WZ, Zhou HB, Bi HH, Fromm M, Yang B, Weeks DP (2013) Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Res 41:188 - PubMed
  2. Liu G, Li J, Godwin ID (2019) Genome editing by CRISPR/Cas9 in sorghum through biolistic bombardment. In: Zhao Z-Y, Dahlberg J (eds) Sorghum: methods and protocols. Springer, New York, pp 169–183. https://doi.org/10.1007/978-1-4939-9039-9_12 - PubMed
  3. Long L, Guo DD, Gao W, Yang WW, Hou LP, Ma XN, Miao YC, Botella JR, Song CP (2018) Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression. Plant Methods 14:85 - PubMed
  4. Mace E, Tai S, Gilding E et al (2013) Whole-genome sequencing reveals untapped genetic potential in Africa’s indigenous cereal crop sorghum. Nat Commun 4:2320. https://doi.org/10.1038/ncomms3320 - PubMed
  5. Peng C, Wang H, Xu X, Wang X, Chen X, Wei W, Lai Y, Liu G, Godwin ID, Li J, Zhang L, Xu J (2018) High-throughput detection and screening of plants modified by gene editing using quantitative real-time polymerase chain reaction. Plant J 95:557–567 - PubMed
  6. Ren C, Liu Y, Guo Y, Duan W, Fan P, Li S, Liang Z (2021) Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters. Hortic Res 8:52 - PubMed
  7. Wu X, Kriz AJ, Sharp PA (2014) Target specificity of the CRISPR-Cas9 system. Quant Biol 2:59–70 - PubMed
  8. Zhan X, Lu Y, Zhu JK, Botella JR (2021) Genome editing for plant research and crop improvement. J Integr Plant Biol 63:3–33 - PubMed
  9. Zhang Y, Massel K, Godwin ID, Gao C (2018) Applications and potential of genome editing in crop improvement. Genome Biol 19:210 - PubMed

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