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DeLuca KF, Mick JE, Ide AH, et al. Generation and diversification of recombinant monoclonal antibodies. Elife. 2021;10doi: 10.7554/eLife.72093.
DeLuca, K. F., Mick, J. E., Ide, A. H., Lima, W. C., Sherman, L., Schaller, K. L., Anderson, S. M., Zhao, N., Stasevich, T. J., Varma, D., Nilsson, J., & DeLuca, J. G. (2021). Generation and diversification of recombinant monoclonal antibodies. eLife, 10. https://doi.org/10.7554/eLife.72093
DeLuca, Keith F, et al. "Generation and diversification of recombinant monoclonal antibodies." eLife vol. 10 (2021). doi: https://doi.org/10.7554/eLife.72093
DeLuca KF, Mick JE, Ide AH, Lima WC, Sherman L, Schaller KL, Anderson SM, Zhao N, Stasevich TJ, Varma D, Nilsson J, DeLuca JG. Generation and diversification of recombinant monoclonal antibodies. Elife. 2021 Dec 31;10. doi: 10.7554/eLife.72093. Epub 2021 Dec 31. PMID: 34970967.
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Elife. 2021 Dec 31;10. doi: 10.7554/eLife.72093. Epub 2021 Dec 31.

Generation and diversification of recombinant monoclonal antibodies.

eLife

Keith F DeLuca, Jeanne E Mick, Amy Hodges Ide, Wanessa C Lima, Lori Sherman, Kristin L Schaller, Steven M Anderson, Ning Zhao, Timothy J Stasevich, Dileep Varma, Jakob Nilsson, Jennifer G DeLuca

Affiliations

  1. Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, United States.
  2. Geneva Antibody Facility, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
  3. CU Cancer Center Cell Technologies Shared Resource, University of Colorado Anschutz Medical Campus, Aurora, United States.
  4. Department of Pediatric Hematology, Oncology and Bone Marrow Transplant, University of Colorado Anschutz Medical Campus, Aurora, United States.
  5. Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, United States.
  6. Department of Cell and Developmental Biology, Northwestern University, Chicago, United States.
  7. The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark.

PMID: 34970967 DOI: 10.7554/eLife.72093

Abstract

Antibodies are indispensable tools used for a large number of applications in both foundational and translational bioscience research; however, there are drawbacks to using traditional antibodies generated in animals. These include a lack of standardization leading to problems with reproducibility, high costs of antibodies purchased from commercial sources, and ethical concerns regarding the large number of animals used to generate antibodies. To address these issues, we have developed practical methodologies and tools for generating low-cost, high-yield preparations of recombinant monoclonal antibodies and antibody fragments directed to protein epitopes from primary sequences. We describe these methods here, as well as approaches to diversify monoclonal antibodies, including customization of antibody species specificity, generation of genetically encoded small antibody fragments, and conversion of single chain antibody fragments (

© 2021, DeLuca et al.

Keywords: biochemistry; cell biology; chemical biology; human

Conflict of interest statement

KD, JM, AI, WL, LS, KS, SA, NZ, TS, DV, JN The other authors declare that no competing interests exist. JD Jennifer G DeLuca, Reviewing editor, eLife.

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Grant support