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Panariello BHD, Cavichioli EAM, Sochacki SF, et al. Effect of blue light plus chlorhexidine therapy on Streptococcus mutans biofilm and its regrowth in an in vitro orthodontic model. Am J Orthod Dentofacial Orthop. 2021;161(1):103-114doi: 10.1016/j.ajodo.2020.06.050.
Panariello, B. H. D., Cavichioli, E. A. M., Sochacki, S. F., Gandini Junior, L. G., & Duarte, S. (2022). Effect of blue light plus chlorhexidine therapy on Streptococcus mutans biofilm and its regrowth in an in vitro orthodontic model. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics, 161(1), 103-114. https://doi.org/10.1016/j.ajodo.2020.06.050
Panariello, Beatriz H D, et al. "Effect of blue light plus chlorhexidine therapy on Streptococcus mutans biofilm and its regrowth in an in vitro orthodontic model." American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics vol. 161,1 (2022): 103-114. doi: https://doi.org/10.1016/j.ajodo.2020.06.050
Panariello BHD, Cavichioli EAM, Sochacki SF, Gandini Junior LG, Duarte S. Effect of blue light plus chlorhexidine therapy on Streptococcus mutans biofilm and its regrowth in an in vitro orthodontic model. Am J Orthod Dentofacial Orthop. 2022 Jan;161(1):103-114. doi: 10.1016/j.ajodo.2020.06.050. Epub 2021 Aug 25. PMID: 34452788.
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Am J Orthod Dentofacial Orthop. 2022 Jan;161(1):103-114. doi: 10.1016/j.ajodo.2020.06.050. Epub 2021 Aug 25.

Effect of blue light plus chlorhexidine therapy on Streptococcus mutans biofilm and its regrowth in an in vitro orthodontic model.

American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics

Beatriz H D Panariello, Eder A M Cavichioli, Sabrina Feitosa Sochacki, Luiz Gonzaga Gandini Junior, Simone Duarte

Affiliations

  1. Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, Indianapolis, Ind.
  2. Department of Orthodontics and Pediatric Dentistry, School of Dentistry at Araraquara, UNESP São Paulo State University, Araraquara, São Paulo, Brazil.
  3. Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, Indianapolis, Ind.
  4. Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, Indianapolis, Ind. Electronic address: [email protected].

PMID: 34452788 DOI: 10.1016/j.ajodo.2020.06.050

Abstract

INTRODUCTION: Fixed orthodontic appliances create areas of stagnation for dental biofilms and make it difficult to clean the teeth; therefore, there is a risk of developing incipient caries lesions during the orthodontic treatment. The objective of this study is to determine if the combination of 2 different therapies, phototherapy by blue light (BL) and the antimicrobial 0.12% chlorhexidine (CHX) on enamel, orthodontic brackets, and elastics, would reduce or inhibit mature Streptococcus mutans biofilms and their regrowth on these substrates 24 hours after the application of the treatment; and if this treatment would interfere with bracket adhesion to the enamel.

METHODS: Biofilms of S. mutans UA159 were formed for 5-days over samples composed of a bovine enamel, orthodontic bracket, and orthodontic elastic. Then, the specimens were treated with 0.89% NaCl for 1 minute, BL for 12 minutes (72 J/cm

RESULTS: After 5 days of biofilm formation, BL+CHX significantly reduced the bacterial viability on enamel compared with NaCl (P = 0.004) and BL (P = 0.014). For bracket and elastic, all the treatments resulted in similar bacterial viability (P ≥0.081). In the regrowth, CHX and BL+CHX significantly reduced the bacterial viability in the enamel compared with the NaCl (P ≤0.015) and BL (P ≤0.013). For bracket, BL+CHX significantly reduced the bacterial viability compared with NaCl (P = 0.008) and BL (P = 0.009). For the elastic, BL+CHX eliminated the biofilms from the substrate. CHX and BL+CHX significantly reduced the bacterial viability 24 hours after treatment for all substrates (P ≤0.05). The media pH significantly increased when samples were treated with CHX and BL+CHX (P ≤0.001). Confocal laser scanning microscopy images visually showed an abundant quantity of red cells in the samples treated with BL+CHX. There was no difference in the SBS between the treatments (P ≥0.932).

CONCLUSIONS: The association between BL and CHX reduced S. mutans biofilm and its regrowth on an in vitro orthodontic model and did not influence the bonding strength between bracket and enamel.

Copyright © 2021 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

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