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Shahgond L, Patel C, Thakur K, et al. Therapeutic potential of probiotics - Lactobacillus plantarum UBLP40 and Bacillus clausii UBBC07 on thioacetamide-induced acute hepatic encephalopathy in rats. Metab Brain Dis. 2021;37(1):185-195doi: 10.1007/s11011-021-00862-w.
Shahgond, L., Patel, C., Thakur, K., Sarkar, D., Acharya, S., & Patel, P. (2022). Therapeutic potential of probiotics - Lactobacillus plantarum UBLP40 and Bacillus clausii UBBC07 on thioacetamide-induced acute hepatic encephalopathy in rats. Metabolic brain disease, 37(1), 185-195. https://doi.org/10.1007/s11011-021-00862-w
Shahgond, Lalita, et al. "Therapeutic potential of probiotics - Lactobacillus plantarum UBLP40 and Bacillus clausii UBBC07 on thioacetamide-induced acute hepatic encephalopathy in rats." Metabolic brain disease vol. 37,1 (2022): 185-195. doi: https://doi.org/10.1007/s11011-021-00862-w
Shahgond L, Patel C, Thakur K, Sarkar D, Acharya S, Patel P. Therapeutic potential of probiotics - Lactobacillus plantarum UBLP40 and Bacillus clausii UBBC07 on thioacetamide-induced acute hepatic encephalopathy in rats. Metab Brain Dis. 2022 Jan;37(1):185-195. doi: 10.1007/s11011-021-00862-w. Epub 2021 Nov 03. PMID: 34731397.
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Metab Brain Dis. 2022 Jan;37(1):185-195. doi: 10.1007/s11011-021-00862-w. Epub 2021 Nov 03.

Therapeutic potential of probiotics - Lactobacillus plantarum UBLP40 and Bacillus clausii UBBC07 on thioacetamide-induced acute hepatic encephalopathy in rats.

Metabolic brain disease

Lalita Shahgond, Chirag Patel, Khushboo Thakur, Dipta Sarkar, Sanjeev Acharya, Priyanshi Patel

Affiliations

  1. Department of Pharmacology, S.S.R. College of Pharmacy, Silvassa, Dadra and Nagar Haveli, India, 396230.
  2. Department of Pharmacology, L. M. College of Pharmacy, Ahmedabad, 380009, India. [email protected].

PMID: 34731397 DOI: 10.1007/s11011-021-00862-w

Abstract

PURPOSE: Hepatic encephalopathy (HE) or hepatic coma is a demanding, not utterly understood complication of acute and chronic liver dysfunction and portosystemic shunting. In HE, hyperammonemia and inflammatory responses are believed to act in synergism. Probiotics, Lactobacillus plantarum UBLP40 and Bacillus clausii UBBC07 reduce small intestinal bacterial overgrowth and hyperammonemia, thereby preventing HE development.

METHODS: The effect of probiotics-Lactobacillus plantarum UBLP40 (10

RESULTS: In contrast to only thioacetamide treated rats, probiotics treatment substantially (p < 0.001) reduced liver function parameters, i.e. serum AST, ALT, ALP, and ammonia, improved behaviour parameters, i.e. decreased motor disruption, improved memory impairment. Probiotics treated rats have also shown a substantial improvement in oxidative stress parameters i.e. reduced lipid peroxidation and increased glutathione level in brain tissue and ameliorated the histopathological changes induced by thioacetamide in the brain and liver.

CONCLUSIONS: It can be concluded based on the findings that the combination therapy of Lactobacillus plantarum UBLP40 and Bacillus clausiiUBBC07 proves to be effective in acute hepatic encephalopathy in the preclinical stage, and further studies are required to assess this therapy potential in the clinical setting.

© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords: Bacillus clausii; Hepatic Encephalopathy; Lactobacillus plantarum; Probiotics; Thioacetamide

References

  1. Afifi N, Ramadan A, El-Eraky W et al (2016) Quercetin protects against thioacetamide-induced hepatotoxicity in rats through decreased oxidative stress biomarkers, the inflammatory cytokines; (TNF-α), (NF-κ B) and DNA fragmentation. Der Pharma Chem 8:48–55 - PubMed
  2. Agarwal AN, Mais DD (2019) Sensitivity and Specificity of Alzheimer Type II Astrocytes in Hepatic Encephalopathy. Arch Pathol Lab Med 143:1256–1258. https://doi.org/10.5858/arpa.2018-0455-OA - PubMed
  3. Aldridge DR, Tranah EJ, Shawcross DL (2015) Pathogenesis of hepatic encephalopathy: role of ammonia and systemic inflammation. J Clin Exp Hepatol 5:S7–S20. https://doi.org/10.1016/j.jceh.2014.06.004 - PubMed
  4. Auron A, Brophy PD (2012) Hyperammonemia in review: pathophysiology, diagnosis, and treatment. Pediatr Nephrol 27:207–222. https://doi.org/10.1007/s00467-011-1838-5 - PubMed
  5. Azhari H, Swain MG (2018) Role of Peripheral Inflammation in Hepatic Encephalopathy. J Clin Exp Hepatol 8:281–285. https://doi.org/10.1016/j.jceh.2018.06.008 - PubMed
  6. Ballan R, Battistini C, Xavier-Santos D, Saad SMI (2020) Interactions of probiotics and prebiotics with the gut microbiota. Prog Mol Biol Transl Sci 171:265–300. https://doi.org/10.1016/bs.pmbts.2020.03.008 - PubMed
  7. Chen WC, Quigley EMM (2014) Probiotics, prebiotics & synbiotics in small intestinal bacterial overgrowth: opening up a new therapeutic horizon! Indian J Med Res 140:582–584 - PubMed
  8. Chen T-M, Subeq Y-M, Lee R-P et al (2008) Single-dose intravenous thioacetamide administration as a model of acute liver damage in rats. Int J Exp Pathol 89:223–231. https://doi.org/10.1111/j.1365-2613.2008.00576.x - PubMed
  9. Cooper AJ (2001) Role of glutamine in cerebral nitrogen metabolism and ammonia neurotoxicity. Ment Retard Dev Disabil Res Rev 7:280–286. https://doi.org/10.1002/mrdd.1039 - PubMed
  10. da Silva SS, Vitolo M, González JMD, de OliveiraS, RP (2014) Overview of Lactobacillus plantarum as a promising bacteriocin producer among lactic acid bacteria. Food Res Int 64:527–536. https://doi.org/10.1016/j.foodres.2014.07.041 - PubMed
  11. Dalal R, McGee RG, Riordan SM, Webster AC (2017) Probiotics for people with hepatic encephalopathy. Cochrane database Syst Rev 2:CD008716. https://doi.org/10.1002/14651858.CD008716.pub3 - PubMed
  12. Danduga RCSR, Dondapati SR, Kola PK et al (2018) Neuroprotective activity of tetramethylpyrazine against 3-nitropropionic acid induced Huntington’s disease-like symptoms in rats. Biomed Pharmacother 105:1254–1268. https://doi.org/10.1016/j.biopha.2018.06.079 - PubMed
  13. Diether NE, Willing BP (2019) Microbial Fermentation of Dietary Protein: An Important Factor in Diet - PubMed
  14. Duc LH, Hong HA, Barbosa TM et al (2004) Characterization of Bacillus probiotics available for human use. Appl Environ Microbiol 70:2161–2171. https://doi.org/10.1128/AEM.70.4.2161-2171.2004 - PubMed
  15. El-Baz FK, Salama A, Salama RAA (2019) Therapeutic Effect of Dunaliella salina Microalgae on Thioacetamide- (TAA-) Induced Hepatic Liver Fibrosis in Rats: Role of TGF-β and MMP9. Biomed Res Int 2019:7028314. https://doi.org/10.1155/2019/7028314 - PubMed
  16. El-Baz FK, Salama AAA, Hussein RA (2020) Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats. Toxicol Reports 7:36–45. https://doi.org/10.1016/j.toxrep.2019.10.017 - PubMed
  17. El-Baz FK, Elgohary R, Salama A (2021) Amelioration of Hepatic Encephalopathy Using Dunaliella salina Microalgae in Rats: Modulation of Hyperammonemia/TLR4. Biomed Res Int 2021:8843218. https://doi.org/10.1155/2021/8843218 - PubMed
  18. El-Marasy SA, El Awdan SA, Abd-Elsalam RM (2019) Protective role of chrysin on thioacetamide-induced hepatic encephalopathy in rats. Chem Biol Interact 299:111–119. https://doi.org/10.1016/j.cbi.2018.11.021 - PubMed
  19. Ennaceur A, Delacour J (1988) A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data. Behav Brain Res 31:47–59. https://doi.org/10.1016/0166-4328(88)90157-x - PubMed
  20. Farjam M, Dehdab P, Abbassnia F et al (2012) Thioacetamide-induced acute hepatic encephalopathy in rat: behavioral, biochemical and histological changes. Iran Red Crescent Med J 14(3):164–170 - PubMed
  21. Giannini EG, Testa R, Savarino V (2005) Liver enzyme alteration: a guide for clinicians. CMAJ 172(3):367–379. https://doi.org/10.1503/cmaj.1040752 - PubMed
  22. Hadjihambi A, Arias N, Sheikh M, Jalan R (2018) Hepatic encephalopathy: a critical current review. Hepatol Int 12:135–147. https://doi.org/10.1007/s12072-017-9812-3 - PubMed
  23. Hernández-Gómez JG, López-Bonilla A, Trejo-Tapia G et al (2021) In vitro bile salt hydrolase (Bsh) activity screening of different probiotic microorganisms. Foods 10:1–10. https://doi.org/10.3390/foods10030674 - PubMed
  24. Jaeger V, DeMorrow S, McMillin M (2019) The Direct Contribution of Astrocytes and Microglia to the Pathogenesis of Hepatic Encephalopathy. J Clin Transl Hepatol 7:352–361. https://doi.org/10.14218/JCTH.2019.00025 - PubMed
  25. Kornerup LS, Gluud LL, Vilstrup H, Dam G (2018) Update on the Therapeutic Management of Hepatic Encephalopathy. Curr Gastroenterol Rep 20:21. https://doi.org/10.1007/s11894-018-0627-8 - PubMed
  26. Li S, Tan H-Y, Wang N et al (2015) The Role of Oxidative Stress and Antioxidants in Liver Diseases. Int J Mol Sci 16:26087–26124. https://doi.org/10.3390/ijms161125942 - PubMed
  27. Liu Y, Tran DQ, Rhoads JM (2018) Probiotics in Disease Prevention and Treatment. J Clin Pharmacol 58(Suppl 1):S164–S179. https://doi.org/10.1002/jcph.1121 - PubMed
  28. Mostafa RE, Salama AAA, Abdel-Rahman RF, Ogaly HA (2017) Hepato- and neuro-protective influences of biopropolis on thioacetamide-induced acute hepatic encephalopathy in rats. Can J Physiol Pharmacol 95:539–547. https://doi.org/10.1139/cjpp-2016-0433 - PubMed
  29. Muramatsu K (1967) Direct Colorimetric Method for the Determination of Free Ammonia in Blood. Agric Biol Chem 31:301–308. https://doi.org/10.1080/00021369.1967.10858803 - PubMed
  30. Parekh PJ, Balart LA (2015) Ammonia and Its Role in the Pathogenesis of Hepatic Encephalopathy. Clin Liver Dis 19:529–537. https://doi.org/10.1016/j.cld.2015.05.002 - PubMed
  31. Patel C, Patel P, Acharya S (2020) Therapeutic Prospective of a Spore-Forming Probiotic-Bacillus clausii UBBC07 Against Acetaminophen-Induced Uremia in Rats. Probiotics Antimicrob Proteins 12:253–258. https://doi.org/10.1007/s12602-019-09540-x - PubMed
  32. Prakash R, Mullen KD (2010) Mechanisms, diagnosis and management of hepatic encephalopathy. Nat Rev Gastroenterol Hepatol 7:515–525. https://doi.org/10.1038/nrgastro.2010.116 - PubMed
  33. Ruiz-Larrea MB, Leal AM, Liza M et al (1994) Antioxidant effects of estradiol and 2-hydroxyestradiol on iron-induced lipid peroxidation of rat liver microsomes. Steroids 59:383–388. https://doi.org/10.1016/0039-128x(94)90006-x - PubMed
  34. Savy N, Brossier D, Brunel-Guitton C et al (2018) Acute pediatric hyperammonemia: current diagnosis and management strategies. Hepat Med 10:105–115. https://doi.org/10.2147/HMER.S140711 - PubMed
  35. Suárez I, Bodega G, Fernández B (2002) Glutamine synthetase in brain: effect of ammonia. Neurochem Int 41:123–142. https://doi.org/10.1016/s0197-0186(02)00033-5 - PubMed
  36. Swaminathan M, Ellul MA, Cross TJ (2018) Hepatic encephalopathy: current challenges and future prospects. Hepat Med 10:1–11. https://doi.org/10.2147/HMER.S118964 - PubMed
  37. Vijitruth R, Liu M, Choi D-Y et al (2006) Cyclooxygenase-2 mediates microglial activation and secondary dopaminergic cell death in the mouse MPTP model of Parkinson’s disease. J Neuroinflammation 3:6. https://doi.org/10.1186/1742-2094-3-6 - PubMed
  38. Vorhees CV, Williams MT (2014) Assessing spatial learning and memory in rodents. ILAR J 55:310–332. https://doi.org/10.1093/ilar/ilu013 - PubMed
  39. Yoon H, Lee JG, Yoo JH et al (2013) Effects of metabolic syndrome on fibrosis in chronic viral hepatitis. Gut Liver 7:469–474. https://doi.org/10.5009/gnl.2013.7.4.469 - PubMed
  40. You DD, Choi GS, Kim JM et al (2017) Long-term Outcomes for Liver Transplant Recipients in Terms of Hepatic Encephalopathy. Transplant Proc 49:1425–1429. https://doi.org/10.1016/j.transproceed.2017.02.054 - PubMed

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