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Daoudi K, Malosse C, Lafnoune A, et al. Mass spectrometry-based top-down and bottom-up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom. FEBS Open Bio. 2021;11(7):1867-1892doi: 10.1002/2211-5463.13143.
Daoudi, K., Malosse, C., Lafnoune, A., Darkaoui, B., Chakir, S., Sabatier, J. M., Chamot-Rooke, J., Cadi, R., & Oukkache, N. (2021). Mass spectrometry-based top-down and bottom-up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom. FEBS open bio, 11(7), 1867-1892. https://doi.org/10.1002/2211-5463.13143
Daoudi, Khadija, et al. "Mass spectrometry-based top-down and bottom-up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom." FEBS open bio vol. 11,7 (2021): 1867-1892. doi: https://doi.org/10.1002/2211-5463.13143
Daoudi K, Malosse C, Lafnoune A, Darkaoui B, Chakir S, Sabatier JM, Chamot-Rooke J, Cadi R, Oukkache N. Mass spectrometry-based top-down and bottom-up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom. FEBS Open Bio. 2021 Jul;11(7):1867-1892. doi: 10.1002/2211-5463.13143. Epub 2021 May 28. PMID: 33715301; PMCID: PMC8255848.
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FEBS Open Bio. 2021 Jul;11(7):1867-1892. doi: 10.1002/2211-5463.13143. Epub 2021 May 28.

Mass spectrometry-based top-down and bottom-up approaches for proteomic analysis of the Moroccan Buthus occitanus scorpion venom.

FEBS open bio

Khadija Daoudi, Christian Malosse, Ayoub Lafnoune, Bouchra Darkaoui, Salma Chakir, Jean-Marc Sabatier, Julia Chamot-Rooke, Rachida Cadi, Naoual Oukkache

Affiliations

  1. Laboratory of Venoms and Toxins, Pasteur Institute of Morocco, Casablanca, Morocco.
  2. Laboratory of Molecular Genetics, Physiopathology and Biotechnology, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Morocco.
  3. Mass spectrometry for Biology Unit, Institut Pasteur, CNRS USR 2000, Paris, France.
  4. Laboratory INSERM UMR 1097, University of Aix Marseille, France.

PMID: 33715301 PMCID: PMC8255848 DOI: 10.1002/2211-5463.13143

Abstract

Buthus occitanus (B. occitanus) is one of the most dangerous scorpions in the world. Despite the involvement of B. occitanus scorpion in severe cases of envenomation in Morocco, no study has focused yet on the proteomic composition of the Moroccan B. occitanus scorpion venom. Mass spectrometry-based proteomic techniques are commonly used in the study of scorpion venoms. The implementation of top-down and bottom-up approaches for proteomic analyses facilitates screening by allowing a global view of the structural aspects of such complex matrices. Here, we provide a partial overview of the venom of B. occitanus scorpion, in order to explore the diversity of its toxins and hereafter understand their effects. To this end, a combination of top-down and bottom-up approaches was applied using nano-high liquid chromatography coupled to nano-electrospray tandem mass spectrometry (nano-LC-ESI MS/MS). The LC-MS results showed that B. occitanus venom contains around 200 molecular masses ranging from 1868 to 16 720 Da, the most representative of which are those between 5000 and 8000 Da. Interestingly, combined top-down and bottom-up LC-MS/MS results allowed the identification of several toxins, which were mainly those acting on ion channels, including those targeting sodium (NaScTxs), potassium (KScTxs), chloride (ClScTxs), and calcium channels (CaScTx), as well as antimicrobial peptides (AMPs), amphipathic peptides, myotropic neuropeptides, and hypothetical secreted proteins. This study reveals the molecular diversity of B. occitanus scorpion venom and identifies components that may have useful pharmacological activities.

© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

Keywords: Buthus occitanus scorpion; bottom-up; top-down; toxins; venom; venomic

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