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Probiotics Antimicrob Proteins. 2021 Oct;13(5):1458-1466. doi: 10.1007/s12602-021-09823-2. Epub 2021 Jul 20.

Purification and Characterization of a New Halocin HA4 from Haloferax larsenii HA4 Isolated from a Salt Lake.

Probiotics and antimicrobial proteins

Ramanjeet Kaur, Santosh Kumar Tiwari

Affiliations

  1. Department of Genetics, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
  2. Department of Genetics, Maharshi Dayanand University, Rohtak, 124001, Haryana, India. [email protected].

PMID: 34286419 DOI: 10.1007/s12602-021-09823-2

Abstract

Halocins are antimicrobial peptides secreted by different members of haloarchaea. Halocin HA4 was purified from Haloferax larsenii HA4 using combination of ultrafiltration and chromatographic techniques. It was found to be ~ 14 kDa with unique N-terminal sequence, H

© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords: Anion-exchange chromatography; FTIR; Gel filtration; Haloarchaea; Halocin; Mode of action

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