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Lipovšek S, Novak T, Dariš B, et al. Ultrastructure of spherites in the midgut diverticula and Malpighian tubules of the harvestman Amilenus aurantiacus during the winter diapause. Histochem Cell Biol. 2021;157(1):107-118doi: 10.1007/s00418-021-02046-0.
Lipovšek, S., Novak, T., Dariš, B., Hofer, F., Leitinger, G., & Letofsky-Papst, I. (2022). Ultrastructure of spherites in the midgut diverticula and Malpighian tubules of the harvestman Amilenus aurantiacus during the winter diapause. Histochemistry and cell biology, 157(1), 107-118. https://doi.org/10.1007/s00418-021-02046-0
Lipovšek, Saška, et al. "Ultrastructure of spherites in the midgut diverticula and Malpighian tubules of the harvestman Amilenus aurantiacus during the winter diapause." Histochemistry and cell biology vol. 157,1 (2022): 107-118. doi: https://doi.org/10.1007/s00418-021-02046-0
Lipovšek S, Novak T, Dariš B, Hofer F, Leitinger G, Letofsky-Papst I. Ultrastructure of spherites in the midgut diverticula and Malpighian tubules of the harvestman Amilenus aurantiacus during the winter diapause. Histochem Cell Biol. 2022 Jan;157(1):107-118. doi: 10.1007/s00418-021-02046-0. Epub 2021 Nov 05. PMID: 34738145.
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Histochem Cell Biol. 2022 Jan;157(1):107-118. doi: 10.1007/s00418-021-02046-0. Epub 2021 Nov 05.

Ultrastructure of spherites in the midgut diverticula and Malpighian tubules of the harvestman Amilenus aurantiacus during the winter diapause.

Histochemistry and cell biology

Saška Lipovšek, Tone Novak, Barbara Dariš, Ferdinand Hofer, Gerd Leitinger, Ilse Letofsky-Papst

Affiliations

  1. Faculty of Medicine, University of Maribor, Taborska ulica 8, SI-2000, Maribor, Slovenia. [email protected].
  2. Department of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška cesta 160, SI-2000, Maribor, Slovenia. [email protected].
  3. Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, SI-2000, Maribor, Slovenia. [email protected].
  4. Gottfried Schatz Research Center, Division of Cell Biology, Histology and Embryology, Medical University of Graz, Neue Stiftingtalstrasse 6, 8010, Graz, Austria. [email protected].
  5. Department of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, Koroška cesta 160, SI-2000, Maribor, Slovenia.
  6. Faculty of Medicine, University of Maribor, Taborska ulica 8, SI-2000, Maribor, Slovenia.
  7. Institute of Electron Microscopy and Nanoanalysis and Center for Electron Microscopy, Graz University of Technology, NAWI Graz, Steyrergasse 17, 8010, Graz, Austria.
  8. Gottfried Schatz Research Center, Division of Cell Biology, Histology and Embryology, Medical University of Graz, Neue Stiftingtalstrasse 6, 8010, Graz, Austria.

PMID: 34738145 DOI: 10.1007/s00418-021-02046-0

Abstract

Amilenus aurantiacus overwinter in diapause, a natural starvation period, in hypogean habitats. The structure of spherites in the midgut diverticula (MD) and Malpighian tubules (MT) has been studied comparatively by light microscopy and TEM to detect eventual differences in mineral consumption in the beginning and at the end of the starvation period in these organs (MD and MT) associated with digestive processes. The chemical composition of spherites was examined by combining energy-dispersive X-ray spectroscopy (EDXS), electron energy-loss spectroscopy (EELS) and energy-filtered TEM (EFTEM). The structure of the spherites changed during overwintering in both organs. At the beginning of overwintering, the spherites were composed of densely packed concentric layers of electron-dense and electron-lucent material. In the middle and at the end of overwintering, the electron-lucent layers between the layers of material indicated the loss of some material. The chemical composition of the spherites changed only in the MD; at the beginning of overwintering, these contained Si, O, C and Fe, while later there was no more Fe. In contrast, spherites in the MT were composed of Si, O, C and Ca throughout overwintering. A less intensive exploitation of the MD spherites was probably due to complete cessation of digestive and other cell activity in this organ during the winter diapause; activity of the MT slowed, but continued removing the cell metabolites.

© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords: Analytical electron microscopy; Arachnids; Malpighian tubules; Midgut diverticula; Opiliones; Spherite structure

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