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Fridman Y, Strauss S, Horev G, et al. The root meristem is shaped by brassinosteroid control of cell geometry. Nat Plants. 2021;7(11):1475-1484doi: 10.1038/s41477-021-01014-9.
Fridman, Y., Strauss, S., Horev, G., Ackerman-Lavert, M., Reiner-Benaim, A., Lane, B., Smith, R. S., & Savaldi-Goldstein, S. (2021). The root meristem is shaped by brassinosteroid control of cell geometry. Nature plants, 7(11), 1475-1484. https://doi.org/10.1038/s41477-021-01014-9
Fridman, Y, et al. "The root meristem is shaped by brassinosteroid control of cell geometry." Nature plants vol. 7,11 (2021): 1475-1484. doi: https://doi.org/10.1038/s41477-021-01014-9
Fridman Y, Strauss S, Horev G, Ackerman-Lavert M, Reiner-Benaim A, Lane B, Smith RS, Savaldi-Goldstein S. The root meristem is shaped by brassinosteroid control of cell geometry. Nat Plants. 2021 Nov;7(11):1475-1484. doi: 10.1038/s41477-021-01014-9. Epub 2021 Nov 15. PMID: 34782771; PMCID: PMC8592843.
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Nat Plants. 2021 Nov;7(11):1475-1484. doi: 10.1038/s41477-021-01014-9. Epub 2021 Nov 15.

The root meristem is shaped by brassinosteroid control of cell geometry.

Nature plants

Y Fridman, S Strauss, G Horev, M Ackerman-Lavert, A Reiner-Benaim, B Lane, R S Smith, S Savaldi-Goldstein

Affiliations

  1. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel.
  2. Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
  3. Lorey I. Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion - Israel Institute of Technology, Haifa, Israel.
  4. Clinical Epidemiology Unit, Rambam Health Care Campus, Haifa, Israel.
  5. Department of Computational and Systems Biology, John Innes Centre, Norwich, UK.
  6. Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany. [email protected].
  7. Department of Computational and Systems Biology, John Innes Centre, Norwich, UK. [email protected].
  8. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel. [email protected].

PMID: 34782771 PMCID: PMC8592843 DOI: 10.1038/s41477-021-01014-9

Abstract

Growth extent and direction determine cell and whole-organ architecture. How they are spatio-temporally modulated to control size and shape is not well known. Here we tackled this question by studying the effect of brassinosteroid (BR) signalling on the structure of the root meristem. Quantification of the three-dimensional geometry of thousands of individual meristematic cells across different tissue types showed that the modulation of BR signalling yields distinct changes in growth rate and anisotropy, which affects the time that cells spend in the meristem and has a strong impact on the final root form. By contrast, the hormone effect on cell volume was minor, establishing cell volume as invariant to the effect of BR. Thus, BR has the highest effect on cell shape and growth anisotropy, regulating the overall longitudinal and radial growth of the meristem, while maintaining a coherent distribution of cell sizes. Moving from single-cell quantification to the whole organ, we developed a computational model of radial growth. The simulation demonstrates how differential BR-regulated growth between the inner and outer tissues shapes the meristem and thus explains the non-intuitive outcomes of tissue-specific perturbation of BR signalling. The combined experimental data and simulation suggest that the inner and outer tissues have distinct but coordinated roles in growth regulation.

© 2021. The Author(s).

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