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Elizarev P, Finkl K, Müller J. Distinct requirements for Pho, Sfmbt, and Ino80 for cell survival in Drosophila. Genetics. 2021;219(1)doi: 10.1093/genetics/iyab096.
Elizarev, P., Finkl, K., & Müller, J. (2021). Distinct requirements for Pho, Sfmbt, and Ino80 for cell survival in Drosophila. Genetics, 219(1), . https://doi.org/10.1093/genetics/iyab096
Elizarev, Pavel, et al. "Distinct requirements for Pho, Sfmbt, and Ino80 for cell survival in Drosophila." Genetics vol. 219,1 (2021). doi: https://doi.org/10.1093/genetics/iyab096
Elizarev P, Finkl K, Müller J. Distinct requirements for Pho, Sfmbt, and Ino80 for cell survival in Drosophila. Genetics. 2021 Aug 26;219(1). doi: 10.1093/genetics/iyab096. PMID: 34849913; PMCID: PMC8633127.
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Genetics. 2021 Aug 26;219(1). doi: 10.1093/genetics/iyab096.

Distinct requirements for Pho, Sfmbt, and Ino80 for cell survival in Drosophila.

Genetics

Pavel Elizarev, Katja Finkl, Jürg Müller

Affiliations

  1. Laboratory of Chromatin Biology, Max-Planck Institute of Biochemistry, Martinsried 82152, Germany.

PMID: 34849913 PMCID: PMC8633127 DOI: 10.1093/genetics/iyab096

Abstract

The Drosophila proteins Pleiohomeotic (Pho) and its paralog Pho-like (Phol) are the homologs of the mammalian transcription factor YY1. Pho and Phol are subunits of the Polycomb group protein complex PhoRC and they are also stably associated with the INO80 nucleosome remodeling complex. Drosophila lacking both Pho and Phol arrest development as larvae with small misshaped imaginal discs. The basis of this phenotype is poorly understood. We find that in pho phol mutant animals cells retain the capacity to proliferate but show a high incidence of apoptotic cell death that results in tissue hypoplasia. Clonal analyses establish that cells stringently require Pho and Phol to survive. In contrast, the PhoRC subunit Sfmbt and the ATP-dependent nucleosome remodeling factor Ino80 are not essential for cell viability. Pho and Phol, therefore, execute their critical role for cell survival through mechanisms that do not involve Sfmbt function or INO80 nucleosome remodeling.

© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

Keywords: Drosophila ; Ino80; Pho; Polycomb; Sfmbt; apoptosis

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