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Zorova LD, Demchenko EA, Korshunova GA, et al. Is the Mitochondrial Membrane Potential (∆Ψ) Correctly Assessed? Intracellular and Intramitochondrial Modifications of the ∆Ψ Probe, Rhodamine 123. Int J Mol Sci. 2022;23(1)doi: 10.3390/ijms23010482.
Zorova, L. D., Demchenko, E. A., Korshunova, G. A., Tashlitsky, V. N., Zorov, S. D., Andrianova, N. V., Popkov, V. A., Babenko, V. A., Pevzner, I. B., Silachev, D. N., Plotnikov, E. Y., & Zorov, D. B. (2022). Is the Mitochondrial Membrane Potential (∆Ψ) Correctly Assessed? Intracellular and Intramitochondrial Modifications of the ∆Ψ Probe, Rhodamine 123. International journal of molecular sciences, 23(1), . https://doi.org/10.3390/ijms23010482
Zorova, Ljubava D, et al. "Is the Mitochondrial Membrane Potential (∆Ψ) Correctly Assessed? Intracellular and Intramitochondrial Modifications of the ∆Ψ Probe, Rhodamine 123." International journal of molecular sciences vol. 23,1 (2022). doi: https://doi.org/10.3390/ijms23010482
Zorova LD, Demchenko EA, Korshunova GA, Tashlitsky VN, Zorov SD, Andrianova NV, Popkov VA, Babenko VA, Pevzner IB, Silachev DN, Plotnikov EY, Zorov DB. Is the Mitochondrial Membrane Potential (∆Ψ) Correctly Assessed? Intracellular and Intramitochondrial Modifications of the ∆Ψ Probe, Rhodamine 123. Int J Mol Sci. 2022 Jan 01;23(1). doi: 10.3390/ijms23010482. PMID: 35008907.
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Int J Mol Sci. 2022 Jan 01;23(1). doi: 10.3390/ijms23010482.

Is the Mitochondrial Membrane Potential (∆Ψ) Correctly Assessed? Intracellular and Intramitochondrial Modifications of the ∆Ψ Probe, Rhodamine 123.

International journal of molecular sciences

Ljubava D Zorova, Evgeniya A Demchenko, Galina A Korshunova, Vadim N Tashlitsky, Savva D Zorov, Nadezda V Andrianova, Vasily A Popkov, Valentina A Babenko, Irina B Pevzner, Denis N Silachev, Egor Y Plotnikov, Dmitry B Zorov

Affiliations

  1. A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia.
  2. Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia.
  3. Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119992 Moscow.

PMID: 35008907 DOI: 10.3390/ijms23010482

Abstract

The mitochondrial membrane potential (∆Ψ) is the driving force providing the electrical component of the total transmembrane potential of hydrogen ions generated by proton pumps, which is utilized by the ATP synthase. The role of ∆Ψ is not limited to its role in bioenergetics since it takes part in other important intracellular processes, which leads to the mandatory requirement of the homeostasis of ∆Ψ. Conventionally, ∆Ψ in living cells is estimated by the fluorescence of probes such as rhodamine 123, tetramethylrodamine, etc. However, when assessing the fluorescence, the possibility of the intracellular/intramitochondrial modification of the rhodamine molecule is not taken into account. Such changes were revealed in this work, in which a comparison of normal (astrocytic) and tumor (glioma) cells was conducted. Fluorescent microscopy, flow cytometry, and mass spectrometry revealed significant modifications of rhodamine molecules developing over time, which were prevented by amiodarone apparently due to blocking the release of xenobiotics from the cell and their transformation with the participation of cytochrome P450. Obviously, an important role in these processes is played by the increased retention of rhodamines in tumor cells. Our data require careful evaluation of mitochondrial ∆Ψ potential based on the assessment of the fluorescence of the mitochondrial probe.

Keywords: cancer; cytochrome P450; energetics; esterase; fluorescence; membrane potential; mitochondria; tumor cells

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