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Di Cesare A, Sabatino R, Yang Y, et al. Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments. Environ Pollut. 2021;297:118774doi: 10.1016/j.envpol.2021.118774.
Di Cesare, A., Sabatino, R., Yang, Y., Brambilla, D., Li, P., Fontaneto, D., Eckert, E. M., & Corno, G. (2021). Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments. Environmental pollution (Barking, Essex : 1987), 297118774. https://doi.org/10.1016/j.envpol.2021.118774
Di Cesare, Andrea, et al. "Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments." Environmental pollution (Barking, Essex : 1987) vol. 297 (2021): 118774. doi: https://doi.org/10.1016/j.envpol.2021.118774
Di Cesare A, Sabatino R, Yang Y, Brambilla D, Li P, Fontaneto D, Eckert EM, Corno G. Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments. Environ Pollut. 2021 Dec 30;297:118774. doi: 10.1016/j.envpol.2021.118774. Epub 2021 Dec 30. PMID: 34974089.
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Environ Pollut. 2021 Dec 30;297:118774. doi: 10.1016/j.envpol.2021.118774. Epub 2021 Dec 30.

Contribution of plasmidome, metal resistome and integrases to the persistence of the antibiotic resistome in aquatic environments.

Environmental pollution (Barking, Essex : 1987)

Andrea Di Cesare, Raffaella Sabatino, Ying Yang, Diego Brambilla, Pu Li, Diego Fontaneto, Ester M Eckert, Gianluca Corno

Affiliations

  1. Water Research Institute (IRSA) - MEG Molecular Ecology Group, CNR - National Research Council of Italy, Largo Tonolli 50, 28922, Verbania, Italy. Electronic address: [email protected].
  2. Water Research Institute (IRSA) - MEG Molecular Ecology Group, CNR - National Research Council of Italy, Largo Tonolli 50, 28922, Verbania, Italy.
  3. School of Marine Sciences, Sun Yat-sen University, 519082, Zhuhai, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), 519082, Zhuhai, China; Pearl River Estuary Marine Ecosystem Research Station, Ministry of Education, Zhuhai, 519082, China.

PMID: 34974089 DOI: 10.1016/j.envpol.2021.118774

Abstract

Wastewater treatment plants (WWTPs) are among the main hotspots of antibiotic resistance genes (ARGs) in the environment. Previously, we demonstrated that, by increasing anthropogenic pollution, the antibiotic resistome persisted in the microbial community of rivers and lakes, independently by changes in community composition. In this study, we reanalysed the data to test for the relation of metal resistance genes (MRGs), plasmids, and integrons to the persistence of the antibiotic resistome. The experiment consisted in replicated co-cultures of riverine or lacustrine microbial communities and WWTP effluents in different proportions. Samples before (T0) and after a short period of incubation (TF) were collected and community metagenomic data were obtained by shotgun sequencing. The data were processed to annotate MRGs, plasmids, and integrases. The integrases stabilized in the aquatic environment following the degree of contamination with effluent water (in particular in one site), whereas MRGs and plasmids showed stochastic trajectories. These results confirm the potential correlation between integrons and anthropogenic pollution, and the reliability of intI1 as a pollution marker. Only in one site MRGs, plasmids, and ARGs were correlated, highlighting their partial contribution to the persistence of ARGs in surface waters.

Copyright © 2021 Elsevier Ltd. All rights reserved.

Keywords: Antimicrobial resistance; Integrons; Metal resistance genes; Plasmids

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