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Piola B, Sabbatini M, Gino S, et al. 3D Bioprinting of Gelatin-Xanthan Gum Composite Hydrogels for Growth of Human Skin Cells. Int J Mol Sci. 2022;23(1)doi: 10.3390/ijms23010539.
Piola, B., Sabbatini, M., Gino, S., Invernizzi, M., & Renò, F. (2022). 3D Bioprinting of Gelatin-Xanthan Gum Composite Hydrogels for Growth of Human Skin Cells. International journal of molecular sciences, 23(1), . https://doi.org/10.3390/ijms23010539
Piola, Beatrice, et al. "3D Bioprinting of Gelatin-Xanthan Gum Composite Hydrogels for Growth of Human Skin Cells." International journal of molecular sciences vol. 23,1 (2022). doi: https://doi.org/10.3390/ijms23010539
Piola B, Sabbatini M, Gino S, Invernizzi M, Renò F. 3D Bioprinting of Gelatin-Xanthan Gum Composite Hydrogels for Growth of Human Skin Cells. Int J Mol Sci. 2022 Jan 04;23(1). doi: 10.3390/ijms23010539. PMID: 35008965; PMCID: PMC8745252.
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Int J Mol Sci. 2022 Jan 04;23(1). doi: 10.3390/ijms23010539.

3D Bioprinting of Gelatin-Xanthan Gum Composite Hydrogels for Growth of Human Skin Cells.

International journal of molecular sciences

Beatrice Piola, Maurizio Sabbatini, Sarah Gino, Marco Invernizzi, Filippo Renò

Affiliations

  1. Innovative Research Laboratory for Wound Healing, Health Sciences Department, Medical School, Università del Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy.
  2. Department of Sciences and Technological Innovation, Università del Piemonte Orientale, Via T. Michel 11, 15121 Alessandria, Italy.
  3. Health Science Department, Physical Medicine and Rehabilitation Division, Università del Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy.
  4. Department of Integrated Research and Innovation, Translational Medicine Unit (DAIRI), Hospital "S.S. Antonio e Biagio e Cesare Arrigo", 15121 Alessandria, Italy.

PMID: 35008965 PMCID: PMC8745252 DOI: 10.3390/ijms23010539

Abstract

In recent years, bioprinting has attracted much attention as a potential tool for generating complex 3D biological constructs capable of mimicking the native tissue microenvironment and promoting physiologically relevant cell-cell and cell-matrix interactions. The aim of the present study was to develop a crosslinked 3D printable hydrogel based on biocompatible natural polymers, gelatin and xanthan gum at different percentages to be used both as a scaffold for cell growth and as a wound dressing. The CellInk Inkredible 3D printer was used for the 3D printing of hydrogels, and a glutaraldehyde solution was tested for the crosslinking process. We were able to obtain two kinds of printable hydrogels with different porosity, swelling and degradation time. Subsequently, the printed hydrogels were characterized from the point of view of biocompatibility. Our results showed that gelatin/xanthan-gum bioprinted hydrogels were biocompatible materials, as they allowed both human keratinocyte and fibroblast in vitro growth for 14 days. These two bioprintable hydrogels could be also used as a helpful dressing material.

Keywords: biocompatibility; bioprinting; gelatin; hydrogel; xanthan gum

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