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Ghafarkhani M, Avci CB, Rahbarghazi R, et al. Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy. Sci Rep. 2021;11(1):23984doi: 10.1038/s41598-021-02697-y.
Ghafarkhani, M., Avci, C. B., Rahbarghazi, R., Karimi, A., Sadeghizadeh, M., Zarebkohan, A., & Bani, F. (2021). Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy. Scientific reports, 11(1), 23984. https://doi.org/10.1038/s41598-021-02697-y
Ghafarkhani, Maryam, et al. "Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy." Scientific reports vol. 11,1 (2021): 23984. doi: https://doi.org/10.1038/s41598-021-02697-y
Ghafarkhani M, Avci CB, Rahbarghazi R, Karimi A, Sadeghizadeh M, Zarebkohan A, Bani F. Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy. Sci Rep. 2021 Dec 14;11(1):23984. doi: 10.1038/s41598-021-02697-y. PMID: 34907215; PMCID: PMC8671444.
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Sci Rep. 2021 Dec 14;11(1):23984. doi: 10.1038/s41598-021-02697-y.

Mild hyperthermia induced by gold nanorods acts as a dual-edge blade in the fate of SH-SY5Y cells via autophagy.

Scientific reports

Maryam Ghafarkhani, Cigir Biray Avci, Reza Rahbarghazi, Abbas Karimi, Majid Sadeghizadeh, Amir Zarebkohan, Farhad Bani

Affiliations

  1. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, 516661-4733, Tabriz, Iran.
  2. Department of Medical Biology, Medical Faculty, Ege University, Bornova, 35100, Izmir, Turkey.
  3. Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
  4. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  5. Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
  6. Department of Nanobiotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  7. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, 516661-4733, Tabriz, Iran. [email protected].
  8. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. [email protected].
  9. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, 516661-4733, Tabriz, Iran. [email protected].
  10. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. [email protected].

PMID: 34907215 PMCID: PMC8671444 DOI: 10.1038/s41598-021-02697-y

Abstract

Unraveling unwanted side effects of nanotechnology-based therapies like photothermal therapy (PTT) is vital in translational nanomedicine. Herein, we monitored the relationship between autophagic response at the transcriptional level by using a PCR array and tumor formation ability by colony formation assay in the human neuroblastoma cell line, SH-SY5Y, 48 h after being exposed to two different mild hyperthermia (43 and 48 °C) induced by PTT. In this regard, the promotion of apoptosis and autophagy were evaluated using immunofluorescence imaging and flow cytometry analyses. Protein levels of Ki-67, P62, and LC3 were measured using ELISA. Our results showed that of 86 genes associated with autophagy, the expression of 54 genes was changed in response to PTT. Also, we showed that chaperone-mediated autophagy (CMA) and macroautophagy are stimulated in PTT. Importantly, the results of this study also showed significant changes in genes related to the crosstalk between autophagy, dormancy, and metastatic activity of treated cells. Our findings illustrated that PTT enhances the aggressiveness of cancer cells at 43 °C, in contrast to 48 °C by the regulation of autophagy-dependent manner.

© 2021. The Author(s).

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