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Kuhn JA, Vainchtein ID, Braz J, et al. Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice. Elife. 2021;10doi: 10.7554/eLife.69056.
Kuhn, J. A., Vainchtein, I. D., Braz, J., Hamel, K., Bernstein, M., Craik, V., Dahlgren, M. W., Ortiz-Carpena, J., Molofsky, A. B., Molofsky, A. V., & Basbaum, A. I. (2021). Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice. eLife, 10. https://doi.org/10.7554/eLife.69056
Kuhn, Julia A, et al. "Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice." eLife vol. 10 (2021). doi: https://doi.org/10.7554/eLife.69056
Kuhn JA, Vainchtein ID, Braz J, Hamel K, Bernstein M, Craik V, Dahlgren MW, Ortiz-Carpena J, Molofsky AB, Molofsky AV, Basbaum AI. Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice. Elife. 2021 Oct 15;10. doi: 10.7554/eLife.69056. PMID: 34652270; PMCID: PMC8639143.
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Elife. 2021 Oct 15;10. doi: 10.7554/eLife.69056.

Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice.

eLife

Julia A Kuhn, Ilia D Vainchtein, Joao Braz, Katherine Hamel, Mollie Bernstein, Veronica Craik, Madelene W Dahlgren, Jorge Ortiz-Carpena, Ari B Molofsky, Anna V Molofsky, Allan I Basbaum

Affiliations

  1. Department of Anatomy, University of California San Francisco, San Francisco, United States.
  2. Department of Psychiatry and Behavioral Sciences/Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, United States.
  3. Department of Laboratory Medicine, University of California, San Francisco, San Francisco, United States.

PMID: 34652270 PMCID: PMC8639143 DOI: 10.7554/eLife.69056

Abstract

Peripheral nerve injury-induced neuropathic pain is a chronic and debilitating condition characterized by mechanical hypersensitivity. We previously identified microglial activation via release of colony-stimulating factor 1 (CSF1) from injured sensory neurons as a mechanism contributing to nerve injury-induced pain. Here, we show that intrathecal administration of CSF1, even in the absence of injury, is sufficient to induce pain behavior, but only in male mice. Transcriptional profiling and morphologic analyses after intrathecal CSF1 showed robust immune activation in male but not female microglia. CSF1 also induced marked expansion of lymphocytes within the spinal cord meninges, with preferential expansion of regulatory T-cells (Tregs) in female mice. Consistent with the hypothesis that Tregs actively suppress microglial activation in females, Treg deficient (

© 2021, Kuhn et al.

Keywords: CSF1; Treg; meninges; microglia; mouse; neuroscience; pain; spinal cord

Conflict of interest statement

JK Patent approved on use of CSF1 blockade to treat neuropathic pain (Publication Number WO/2016/057800). IV, JB, KH, MB, VC, MD, JO, AM, AM No competing interests declared, AB Reviewing editor, eLife

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