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Ecklund KH, Bailey ME, Kossen KA, et al. The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity. J Cell Sci. 2021;134(23)doi: 10.1242/jcs.258510.
Ecklund, K. H., Bailey, M. E., Kossen, K. A., Dietvorst, C. K., Asbury, C. L., & Markus, S. M. (2021). The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity. Journal of cell science, 134(23), . https://doi.org/10.1242/jcs.258510
Ecklund, Kari H, et al. "The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity." Journal of cell science vol. 134,23 (2021). doi: https://doi.org/10.1242/jcs.258510
Ecklund KH, Bailey ME, Kossen KA, Dietvorst CK, Asbury CL, Markus SM. The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity. J Cell Sci. 2021 Dec 01;134(23). doi: 10.1242/jcs.258510. Epub 2021 Dec 02. PMID: 34854468; PMCID: PMC8714068.
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J Cell Sci. 2021 Dec 01;134(23). doi: 10.1242/jcs.258510. Epub 2021 Dec 02.

The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity.

Journal of cell science

Kari H Ecklund, Megan E Bailey, Kelly A Kossen, Carsten K Dietvorst, Charles L Asbury, Steven M Markus

Affiliations

  1. Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA.
  2. Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA.

PMID: 34854468 PMCID: PMC8714068 DOI: 10.1242/jcs.258510

Abstract

Dynein motors move the mitotic spindle to the cell division plane in many cell types, including in budding yeast, in which dynein is assisted by numerous factors including the microtubule-associated protein (MAP) She1. Evidence suggests that She1 plays a role in polarizing dynein-mediated spindle movements toward the daughter cell; however, how She1 performs this function is unknown. We find that She1 assists dynein in maintaining the spindle in close proximity to the bud neck, such that, at anaphase onset, the chromosomes are segregated to mother and daughter cells. She1 does so by attenuating the initiation of dynein-mediated spindle movements within the mother cell, thus ensuring such movements are polarized toward the daughter cell. Our data indicate that this activity relies on She1 binding to the microtubule-bound conformation of the dynein microtubule-binding domain, and to astral microtubules within mother cells. Our findings reveal how an asymmetrically localized MAP directionally tunes dynein activity by attenuating motor activity in a spatially confined manner.

© 2021. Published by The Company of Biologists Ltd.

Keywords: Dynactin; Dynein; Microtubule-associated proteins; She1; Spindle positioning

Conflict of interest statement

Competing interests The authors declare no competing or financial interests.

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