Display options
Cite
Henning A, Henkel J, Bier FF, et al. Label-free electrical quantification of the dielectrophoretic response of DNA. PMC Biophys. 2008;1(1):4doi: 10.1186/1757-5036-1-4.
Henning, A., Henkel, J., Bier, F. F., & Hölzel, R. (2008). Label-free electrical quantification of the dielectrophoretic response of DNA. PMC biophysics, 1(1), 4. https://doi.org/10.1186/1757-5036-1-4
Henning, Anja, et al. "Label-free electrical quantification of the dielectrophoretic response of DNA." PMC biophysics vol. 1,1 (2008): 4. doi: https://doi.org/10.1186/1757-5036-1-4
Henning A, Henkel J, Bier FF, Hölzel R. Label-free electrical quantification of the dielectrophoretic response of DNA. PMC Biophys. 2008 Nov 05;1(1):4. doi: 10.1186/1757-5036-1-4. PMID: 19351426; PMCID: PMC2666632.
Copy Download .nbib Format: NLM
Share it on

PMC Biophys. 2008 Nov 05;1(1):4. doi: 10.1186/1757-5036-1-4.

Label-free electrical quantification of the dielectrophoretic response of DNA.

PMC biophysics

Anja Henning, Jörg Henkel, Frank F Bier, Ralph Hölzel

Affiliations

  1. Fraunhofer Institute for Biomedical Engineering, Am Mühlenberg 13, 14476 Potsdam-Golm, Germany. [email protected].

PMID: 19351426 PMCID: PMC2666632 DOI: 10.1186/1757-5036-1-4

Abstract

A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples of 1 muL volume. The method has been applied to the characterisation of the dielectrophoretic response of DNA without the need for any chemical modifications. The influence of electrical parameters like duty cycle, voltage and frequency has been investigated. The results are in good agreement with data from dielectrophoretic studies on fluorescently labelled DNA. Extension of the method down to the single molecule level appears feasible.PACS: 87.50.ch, 87.80.Fe, 87.85.fK.

References

  1. Electrophoresis. 2007 Dec;28(24):4521-38 - PubMed
  2. Biophys J. 1998 Feb;74(2 Pt 1):1024-30 - PubMed
  3. Electrophoresis. 2002 Aug;23(16):2658-66 - PubMed
  4. Nucleic Acids Res. 2004 Jan 22;32(2):e20 - PubMed
  5. Phys Rev E Stat Nonlin Soft Matter Phys. 2002 Aug;66(2 Pt 2):026305 - PubMed
  6. Anal Bioanal Chem. 2003 Oct;377(3):528-39 - PubMed
  7. IEE Proc Nanobiotechnol. 2003 Nov;150(2):47-53 - PubMed
  8. Biophys J. 2002 Oct;83(4):2170-9 - PubMed
  9. Science. 2002 Feb 22;295(5559):1503-6 - PubMed
  10. Biochim Biophys Acta. 1999 May 6;1450(1):53-60 - PubMed
  11. Nanotechnology. 2008 Apr 30;19(17):175202 - PubMed
  12. Anal Bioanal Chem. 2005 Jan;381(1):141-55 - PubMed
  13. Phys Rev Lett. 2005 Sep 16;95(12):128102 - PubMed
  14. Biosens Bioelectron. 2004 Oct 15;20(3):606-19 - PubMed
  15. Science. 1993 Dec 3;262(5139):1561-3 - PubMed
  16. Science. 2003 Sep 26;301(5641):1882-4 - PubMed
  17. Science. 2003 Jul 18;301(5631):344-7 - PubMed
  18. IEEE Trans Nanobioscience. 2006 Mar;5(1):1-8 - PubMed
  19. Nano Lett. 2005 Apr;5(4):777-81 - PubMed
  20. Science. 2003 Nov 7;302(5647):1002-5 - PubMed
  21. Nature. 2006 Mar 16;440(7082):297-302 - PubMed
  22. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12577-82 - PubMed
  23. Nat Biotechnol. 2003 Oct;21(10):1192-9 - PubMed
  24. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 2000 Apr;61(4 Pt B):4011-8 - PubMed
  25. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16677-80 - PubMed
  26. Small. 2008 Apr;4(4):447-50 - PubMed
  27. Eur Phys J E Soft Matter. 2005 Oct;18(2):133-42 - PubMed
  28. Biophys J. 1997 Aug;73(2):1103-9 - PubMed

Publication Types