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Naumann C, Brumm T, Bayerl TM. Phase transition behavior of single phosphatidylcholine bilayers on a solid spherical support studied by DSC, NMR and FT-IR. Biophys J. 1992;63(5):1314-9doi: 10.1016/S0006-3495(92)81708-3.
Naumann, C., Brumm, T., & Bayerl, T. M. (1992). Phase transition behavior of single phosphatidylcholine bilayers on a solid spherical support studied by DSC, NMR and FT-IR. Biophysical journal, 63(5), 1314-9. https://doi.org/10.1016/S0006-3495(92)81708-3
Naumann, C, et al. "Phase transition behavior of single phosphatidylcholine bilayers on a solid spherical support studied by DSC, NMR and FT-IR." Biophysical journal vol. 63,5 (1992): 1314-9. doi: https://doi.org/10.1016/S0006-3495(92)81708-3
Naumann C, Brumm T, Bayerl TM. Phase transition behavior of single phosphatidylcholine bilayers on a solid spherical support studied by DSC, NMR and FT-IR. Biophys J. 1992 Nov;63(5):1314-9. doi: 10.1016/S0006-3495(92)81708-3. PMID: 19431855; PMCID: PMC1261435.
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Biophys J. 1992 Nov;63(5):1314-9. doi: 10.1016/S0006-3495(92)81708-3.

Phase transition behavior of single phosphatidylcholine bilayers on a solid spherical support studied by DSC, NMR and FT-IR.

Biophysical journal

C Naumann, T Brumm, T M Bayerl

Affiliations

  1. Technische Universität München, Physik Department E22, W-8046 Garching, Germany.

PMID: 19431855 PMCID: PMC1261435 DOI: 10.1016/S0006-3495(92)81708-3

Abstract

For the first time, the chain melting transition from the gel phase to the liquid crystalline phase of a single DPPC bilayer on a solid, spherical support (silica beads) is observed by differential scanning calorimetry (DSC). This transition is remarkably cooperative, exhibits a transition temperature T(m) which is 2 degrees C lower than usually found for DPPC multilamellar vesicles and its excess enthalpy is about 25% less than in DPPC multilayers. 31P- and 2H-NMR data as well as FT-IR data provide evidence that despite the highly asymmetric characteristic of the model system, the whole single bilayer undergoes the transition at T(m), i.e., there is no decoupling of the two monolayer leaflets at the main phase transition. Furthermore, our results show that the formation of the ripple (P(beta')) phase is inhibited in single bilayers on a solid support. This result confirms a conclusion which we reached previously on the basis of neutron scattering data obtained on planar supported bilayers. The most likely reason for this inhibition as well as for the above mentioned thermodynamic differences between multilamellar vesicles and supported membranes is a significantly higher lateral stress in the latter. Moreover, the exchange of lipids between two populations of spherical supported vesicles (DMPC and chain perdeuterated DMPC) is studied by DSC. It is shown that this exchange process is symmetric and its half-time is a factor of 3-4 higher than observed for small sonicated DMPC vesicles.

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