Display options
Cite
Raynov AM, Choung YH, Park HY, et al. Establishment and characterization of an in vitro model for cholesteatoma. Clin Exp Otorhinolaryngol. 2008;1(2):86-91doi: 10.3342/ceo.2008.1.2.86.
Raynov, A. M., Choung, Y. H., Park, H. Y., Choi, S. J., & Park, K. (2008). Establishment and characterization of an in vitro model for cholesteatoma. Clinical and experimental otorhinolaryngology, 1(2), 86-91. https://doi.org/10.3342/ceo.2008.1.2.86
Raynov, Alexander M, et al. "Establishment and characterization of an in vitro model for cholesteatoma." Clinical and experimental otorhinolaryngology vol. 1,2 (2008): 86-91. doi: https://doi.org/10.3342/ceo.2008.1.2.86
Raynov AM, Choung YH, Park HY, Choi SJ, Park K. Establishment and characterization of an in vitro model for cholesteatoma. Clin Exp Otorhinolaryngol. 2008 Jun;1(2):86-91. doi: 10.3342/ceo.2008.1.2.86. Epub 2008 Jun 20. PMID: 19434278; PMCID: PMC2671788.
Copy Download .nbib Format: NLM
Share it on

Clin Exp Otorhinolaryngol. 2008 Jun;1(2):86-91. doi: 10.3342/ceo.2008.1.2.86. Epub 2008 Jun 20.

Establishment and characterization of an in vitro model for cholesteatoma.

Clinical and experimental otorhinolaryngology

Alexander M Raynov, Yun-Hoon Choung, Hun Yi Park, Seong Jun Choi, Keehyun Park

Affiliations

  1. Department of Otorhinolaryngology, University Hospital Tzaritza Joanna, Medical University-Sofia, Sofia, Bulgaria.

PMID: 19434278 PMCID: PMC2671788 DOI: 10.3342/ceo.2008.1.2.86

Abstract

OBJECTIVES: Experimental models are of importance to study the pathogenesis of middle ear cholesteatoma, however, they were not established until now. We aimed to develop in vitro model of middle ear cholesteatoma using primary keratinocytes and fibroblasts isolated from cholesteatoma tissue. HaCaT cell line was used as a "skin equivalent" and to compare the grade of homogeneity between cholesteatoma keratinocytes and HaCaT cells.

METHODS: Primary keratinocytes were isolated from cholesteatoma tissue, co-cultured with preliminary prepared feeder layer from cholesteatoma fibroblasts and subsequently air-exposed. The protein profile of cholesteatoma keratinocytes and HaCaT cells was evaluated by means of immunoblot using monoclonal antibody against cytokeratin (CK) 13 and 16. Tissue localization of CK 13 and 16 was accomplished with immunohistochemistry.

RESULTS: Different protein profile and stronger expression of CK 13 and 16 were demonstrated in cholesteatoma keratinocytes in comparison with HaCaT cells. Bigger stratification was observed in the 3D-in vitro systems when both cholesteatoma keratinocytes and HaCaT cells were respectively co-cultured with fibroblasts in comparison with the corresponding control groups without fibroblasts.

CONCLUSION: 3D-model demonstrates the significance of intercellular interaction between components of cholesteatoma tissue.

Keywords: Cholesteatoma; Culture; In vitro; Keratinocytes

References

  1. Am J Otolaryngol. 1988 Jul-Aug;9(4):180-8 - PubMed
  2. J Cell Biol. 1988 Mar;106(3):761-71 - PubMed
  3. Am J Otolaryngol. 1993 Mar-Apr;14(2):82-7 - PubMed
  4. Laryngoscope. 1999 Nov;109(11):1825-31 - PubMed
  5. Am J Otol. 1996 May;17(3):393-6 - PubMed
  6. Proc R Soc Med. 1964 Sep;57:769-71 - PubMed
  7. Acta Otolaryngol. 1983 Jul-Aug;96(1-2):119-29 - PubMed
  8. Acta Otolaryngol. 2002 Oct;122(7):720-5 - PubMed
  9. Clin Otolaryngol Allied Sci. 1983 Jun;8(3):165-70 - PubMed
  10. Clin Otolaryngol Allied Sci. 1995 Oct;20(5):453-60 - PubMed
  11. Am J Otolaryngol. 1989 Nov-Dec;10(6):393-8 - PubMed
  12. Ann Otol Rhinol Laryngol. 1997 Aug;106(8):669-73 - PubMed
  13. Otolaryngol Head Neck Surg. 1994 Mar;110(3):310-7 - PubMed
  14. Acta Otolaryngol. 1996 Mar;116(2):293-8 - PubMed
  15. Laryngoscope. 1999 Jul;109(7 Pt 1):1102-7 - PubMed
  16. Lancet. 1991 Oct 26;338(8774):1041-3 - PubMed
  17. J Invest Dermatol. 1999 Apr;112(4):489-98 - PubMed
  18. Acta Otolaryngol. 1982 Jan-Feb;93(1-2):61-7 - PubMed

Publication Types