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Tchetina EV, Mwale F, Poole AR. Changes in gene expression associated with matrix turnover, chondrocyte proliferation and hypertrophy in the bovine growth plate. Acta Naturae. 2014;6(3):89-97
Tchetina, E. V., Mwale, F., & Poole, A. R. (2014). Changes in gene expression associated with matrix turnover, chondrocyte proliferation and hypertrophy in the bovine growth plate. Acta naturae, 6(3), 89-97.
Tchetina, E V, et al. "Changes in gene expression associated with matrix turnover, chondrocyte proliferation and hypertrophy in the bovine growth plate." Acta naturae vol. 6,3 (2014): 89-97.
Tchetina EV, Mwale F, Poole AR. Changes in gene expression associated with matrix turnover, chondrocyte proliferation and hypertrophy in the bovine growth plate. Acta Naturae. 2014 Jul;6(3):89-97. PMID: 25349717; PMCID: PMC4207563.
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Acta Naturae. 2014 Jul;6(3):89-97.

Changes in gene expression associated with matrix turnover, chondrocyte proliferation and hypertrophy in the bovine growth plate.

Acta naturae

E V Tchetina, F Mwale, A R Poole

Affiliations

  1. Clinical Immunology Department, Research Institute of Rheumatology, Russian Academy of Sciences, Moscow, 115522, Russia.
  2. Orthopaedics Research Laboratory, Jewish General Hospital, Lady Davis Institute for Medical Research, Division of Orthopedic Surgery, McGill University, Montreal, Quebec, H3T 1E2, Canada.
  3. Department of Surgery, McGill University, McGill University, Montreal, Quebec, H3A 0G4, Canada.

PMID: 25349717 PMCID: PMC4207563

Abstract

The aim of the study is to investigate the interrelationships between the expression of genes for structural extracellular matrix molecules, proteinases and their inhibitors in the bovine fetal growth plate. This was analyzed by RT-PCR in microsections of the proximal tibial growth plate of bovine fetuses in relationship to expression of genes associated with chondrocyte proliferation, apoptosis, and matrix vascularization. In the resting zone the genes for extracellular matrix molecule synthesis were expressed. Extracellular matrix degrading enzymes and their inhibitors were also expressed here. Onset of proliferation involved cyclic upregulation of cell division-associated activity and reduced expression of extracellular matrix molecules. Later in the proliferative zone we noted transient expression of proteinases and their inhibitors, extracellular matrix molecules, as well as activity associated with vascularization and apoptosis. With the onset of hypertrophy expression of proteinases and their inhibitors, extracellular matrix molecules, as well as activity associated with vascularization and apoptosis were significantly upregulated. Terminal differentiation was characterized by high expression of proteinases and their inhibitors, extracellular matrix molecules, as well as activity associated with apoptosis. This study reveals the complex interrelationships of gene expression in the physis that accompany matrix assembly, resorption, chondrocyte proliferation, hypertrophy, vascularization and cell death while principal zones of the growth plate are characterized by a distinct signature profile of gene expression.

Keywords: chondrocyte differentiation; gene expression; growth plate; proteinases

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