Display options
Cite
Huk A, Collins AR, El Yamani N, et al. Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay. Mutagenesis. 2015;30(1):85-8doi: 10.1093/mutage/geu077.
Huk, A., Collins, A. R., El Yamani, N., Porredon, C., Azqueta, A., de Lapuente, J., & Dusinska, M. (2015). Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay. Mutagenesis, 30(1), 85-8. https://doi.org/10.1093/mutage/geu077
Huk, Anna, et al. "Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay." Mutagenesis vol. 30,1 (2015): 85-8. doi: https://doi.org/10.1093/mutage/geu077
Huk A, Collins AR, El Yamani N, Porredon C, Azqueta A, de Lapuente J, Dusinska M. Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay. Mutagenesis. 2015 Jan;30(1):85-8. doi: 10.1093/mutage/geu077. PMID: 25527731.
Copy Download .nbib Format: NLM
Share it on

Mutagenesis. 2015 Jan;30(1):85-8. doi: 10.1093/mutage/geu077.

Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay.

Mutagenesis

Anna Huk, Andrew R Collins, Naouale El Yamani, Constanca Porredon, Amaya Azqueta, Joaquín de Lapuente, Maria Dusinska

Affiliations

  1. Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute for Air Research, Instituttveien 18, 2007 Kjeller, Norway.
  2. Department of Nutrition, University of Oslo, Sognsvannsveien 9, 0372 Oslo, Norway.
  3. Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute for Air Research, Instituttveien 18, 2007 Kjeller, Norway, Department of Nutrition, University of Oslo, Sognsvannsveien 9, 0372 Oslo, Norway.
  4. Unit of Experimental Toxicology and Ecotoxicology (UTOX-CERETOX), Barcelona Science Park, Parc Científic de Barcelona c/ Baldiri Reixac, 10-12, Edifici Cluster, 08028 Barcelona, Spain.
  5. Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Navarra, C/Irunlarrea 1, 31009 Pamplona, Spain.
  6. Health Effects Laboratory, Department of Environmental Chemistry, NILU-Norwegian Institute for Air Research, Instituttveien 18, 2007 Kjeller, Norway, [email protected].

PMID: 25527731 DOI: 10.1093/mutage/geu077

Abstract

The comet assay is widely used to test the genotoxicity of engineered nanomaterials (ENMs) but outcomes may vary when results from different laboratories, or even within one laboratory, are compared. We address some basic methodological considerations, such as the importance of carrying out physico-chemical characterisation of the ENMs in test-medium, performing uptake and cytotoxicity tests, and testing several genotoxicity-related endpoints. In this commentary, we discuss the different ways in which concentration of ENMs can be expressed, and stress the need to include appropriate controls and reference standards to monitor variation and avoid interference. Treatment conditions, including cell number, cell culture plate format and volume of treatment medium on the plate are crucial factors that may impact on results and thus should be kept constant within the study.

© The Author 2014. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: [email protected].

MeSH terms

Publication Types