Display options
Cite
Gutte H, Hansen AE, Henriksen ST, et al. Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner. Am J Nucl Med Mol Imaging. 2014;5(1):38-45
Gutte, H., Hansen, A. E., Henriksen, S. T., Johannesen, H. H., Ardenkjaer-Larsen, J., Vignaud, A., Hansen, A. E., Børresen, B., Klausen, T. L., Wittekind, A. M., Gillings, N., Kristensen, A. T., Clemmensen, A., Højgaard, L., & Kjær, A. (2015). Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner. American journal of nuclear medicine and molecular imaging, 5(1), 38-45.
Gutte, Henrik, et al. "Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner." American journal of nuclear medicine and molecular imaging vol. 5,1 (2015): 38-45.
Gutte H, Hansen AE, Henriksen ST, Johannesen HH, Ardenkjaer-Larsen J, Vignaud A, Hansen AE, Børresen B, Klausen TL, Wittekind AM, Gillings N, Kristensen AT, Clemmensen A, Højgaard L, Kjær A. Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner. Am J Nucl Med Mol Imaging. 2014 Dec 15;5(1):38-45. eCollection 2015. PMID: 25625025; PMCID: PMC4299777.
Copy Download .nbib Format: NLM
Share it on

Am J Nucl Med Mol Imaging. 2014 Dec 15;5(1):38-45. eCollection 2015.

Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner.

American journal of nuclear medicine and molecular imaging

Henrik Gutte, Adam E Hansen, Sarah T Henriksen, Helle H Johannesen, Jan Ardenkjaer-Larsen, Alexandre Vignaud, Anders E Hansen, Betina Børresen, Thomas L Klausen, Anne-Mette N Wittekind, Nic Gillings, Annemarie T Kristensen, Andreas Clemmensen, Liselotte Højgaard, Andreas Kjær

Affiliations

  1. Department of Clinical Physiology, Nuclear Medicine and PET, Rigshospitalet, University of Copenhagen Denmark ; Cluster for Molecular Imaging, Faculty of Health Sciences, University of Copenhagen Denmark.
  2. Department of Clinical Physiology, Nuclear Medicine and PET, Rigshospitalet, University of Copenhagen Denmark.
  3. Department of Clinical Physiology, Nuclear Medicine and PET, Rigshospitalet, University of Copenhagen Denmark ; Department of Electrical Engineering, Technical University of Denmark Lyngby, Denmark.
  4. Department of Electrical Engineering, Technical University of Denmark Lyngby, Denmark ; GE Healthcare Brøndby, Denmark.
  5. CEA Saclay, I2BM, NeuroSpin, UNIRS Gif sur Yvette, France.
  6. Cluster for Molecular Imaging, Faculty of Health Sciences, University of Copenhagen Denmark ; Center for Nanomedicine and Theranostics, Technical University of Denmark Denmark.
  7. Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Frederiksberg C, Denmark.

PMID: 25625025 PMCID: PMC4299777

Abstract

In this paper we demonstrate, for the first time, the feasibility of a new imaging concept - combined hyperpolarized (13)C-pyruvate magnetic resonance spectroscopic imaging (MRSI) and (18)F-FDG-PET imaging. This procedure was performed in a clinical PET/MRI scanner with a canine cancer patient. We have named this concept hyper PET. Intravenous injection of the hyperpolarized (13)C-pyruvate results in an increase of (13)C-lactate, (13)C-alanine and (13)C-CO2 ((13)C-HCO3) resonance peaks relative to the tissue, disease and the metabolic state probed. Accordingly, with dynamic nuclear polarization (DNP) and use of (13)C-pyruvate it is now possible to directly study the Warburg Effect through the rate of conversion of (13)C-pyruvate to (13)C-lactate. In this study, we combined it with (18)F-FDG-PET that studies uptake of glucose in the cells. A canine cancer patient with a histology verified local recurrence of a liposarcoma on the right forepaw was imaged using a combined PET/MR clinical scanner. PET was performed as a single-bed, 10 min acquisition, 107 min post injection of 310 MBq (18)F-FDG. (13)C-chemical shift imaging (CSI) was performed just after FDG-PET and 30 s post injection of 23 mL hyperpolarized (13)C-pyruvate. Peak heights of (13)C-pyruvate and (13)C-lactate were quantified using a general linear model. Anatomic (1)H-MRI included axial and coronal T1 vibe, coronal T2-tse and axial T1-tse with fat saturation following gadolinium injection. In the tumor we found clearly increased (13)C-lactate production, which also corresponded to high (18)F-FDG uptake on PET. This is in agreement with the fact that glycolysis and production of lactate are increased in tumor cells compared to normal cells. Yet, most interestingly, also in the muscle of the forepaw of the dog high (18)F-FDG uptake was observed. This was due to activity in these muscles prior to anesthesia, which was not accompanied by a similarly high (13)C-lactate production. Accordingly, this clearly demonstrates how the Warburg Effect directly can be demonstrated by hyperpolarized (13)C-pyruvate MRSI. This was not possible with (18)F-FDG-PET imaging due to inability to discriminate between causes of increased glucose uptake. We propose that this new concept of simultaneous hyperpolarized (13)C-pyruvate MRSI and PET may be highly valuable for image-based non-invasive phenotyping of tumors. This methods may be useful for treatment planning and therapy monitoring.

Keywords: 13C-pyruvate; 18F-FDG-PET; Cancer; DNP; MR; PET/MR; hyperpolarized; molecular imaging; response monitoring

References

  1. Science. 1956 Feb 24;123(3191):309-14 - PubMed
  2. Clin Cancer Res. 2010 Feb 1;16(3):978-85 - PubMed
  3. Nat Med. 2007 Nov;13(11):1382-7 - PubMed
  4. Cancer Metastasis Rev. 2007 Jun;26(2):225-39 - PubMed
  5. Nat Rev Cancer. 2004 Nov;4(11):891-9 - PubMed
  6. Nat Rev Cancer. 2011 Feb;11(2):85-95 - PubMed
  7. Radiother Oncol. 2012 Mar;102(3):424-8 - PubMed
  8. J Magn Reson Imaging. 2012 Dec;36(6):1314-28 - PubMed
  9. J Nucl Med. 2009 May;50 Suppl 1:122S-50S - PubMed
  10. Sci Transl Med. 2013 Aug 14;5(198):198ra108 - PubMed
  11. Cell. 2011 Mar 4;144(5):646-74 - PubMed
  12. PLoS One. 2014 Mar 13;9(3):e91387 - PubMed
  13. Nat Rev Cancer. 2002 Sep;2(9):683-93 - PubMed
  14. Nat Rev Cancer. 2008 Feb;8(2):94-107 - PubMed
  15. J Nucl Med. 2013 Jul;54(7):1113-9 - PubMed
  16. MAGMA. 2013 Feb;26(1):37-47 - PubMed
  17. J Nucl Med. 2011 Oct;52(10 ):1585-600 - PubMed
  18. J Bioenerg Biomembr. 2007 Jun;39(3):223-9 - PubMed
  19. Appl Magn Reson. 2008;34(3-4):533-544 - PubMed
  20. J Am Osteopath Assoc. 2006 Jan;106(1):23-7 - PubMed
  21. Neoplasia. 2009 Jun;11(6):574-82, 1 p following 582 - PubMed
  22. J Magn Reson. 2013 Apr;229:187-97 - PubMed
  23. Science. 2009 May 22;324(5930):1029-33 - PubMed
  24. BMC Cancer. 2013 Apr 01;13:168 - PubMed
  25. Magn Reson Med. 2011 Aug;66(2):505-19 - PubMed
  26. AJR Am J Roentgenol. 2007 Aug;189(2):323-8 - PubMed
  27. Radiat Oncol. 2012 Jun 15;7:89 - PubMed
  28. Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10158-63 - PubMed

Publication Types