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Erdeş E, Doğan TS, Coşar I, et al. Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity. J Venom Anim Toxins Incl Trop Dis. 2014;20(1):48doi: 10.1186/1678-9199-20-48.
Erdeş, E., Doğan, T. S., Coşar, I., Danışman, T., Kunt, K. B., Seker, T., Yücel, M., & Ozen, C. (2014). Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity. The journal of venomous animals and toxins including tropical diseases, 20(1), 48. https://doi.org/10.1186/1678-9199-20-48
Erdeş, Efe, et al. "Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity." The journal of venomous animals and toxins including tropical diseases vol. 20,1 (2014): 48. doi: https://doi.org/10.1186/1678-9199-20-48
Erdeş E, Doğan TS, Coşar I, Danışman T, Kunt KB, Seker T, Yücel M, Ozen C. Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity. J Venom Anim Toxins Incl Trop Dis. 2014 Nov 03;20(1):48. doi: 10.1186/1678-9199-20-48. eCollection 2014. PMID: 25414725; PMCID: PMC4237746.
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J Venom Anim Toxins Incl Trop Dis. 2014 Nov 03;20(1):48. doi: 10.1186/1678-9199-20-48. eCollection 2014.

Characterization of Leiurus abdullahbayrami (Scorpiones: Buthidae) venom: peptide profile, cytotoxicity and antimicrobial activity.

The journal of venomous animals and toxins including tropical diseases

Efe Erdeş, Tuğba Somay Doğan, Ilhan Coşar, Tarık Danışman, Kadir Boğaç Kunt, Tamay Seker, Meral Yücel, Can Ozen

Affiliations

  1. Department of Biotechnology, Graduate School of Natural and Applied Sciences, METU, Ankara, 06800 Turkey ; Molecular Biology and Biotechnology Research and Development Center, Central Laboratory, METU, Ankara, Turkey.
  2. Molecular Biology and Biotechnology Research and Development Center, Central Laboratory, METU, Ankara, Turkey ; Department of Biology, Faculty of Science, Hecettepe University, Ankara, Turkey.
  3. Department of Biology, Faculty of Arts and Sciences, Kirikkale University, Kirikkale, Turkey.
  4. Department of Biology, Faculty of Science, Anadolu University, Eskisehir, Turkey.
  5. Molecular Biology and Biotechnology Research and Development Center, Central Laboratory, METU, Ankara, Turkey.
  6. Molecular Biology and Biotechnology Research and Development Center, Central Laboratory, METU, Ankara, Turkey ; Department of Biological Sciences, Faculty of Arts and Sciences, METU, Ankara, Turkey.
  7. Department of Biotechnology, Graduate School of Natural and Applied Sciences, METU, Ankara, 06800 Turkey ; Molecular Biology and Biotechnology Research and Development Center, Central Laboratory, METU, Ankara, Turkey ; Center of Excellence in Biomaterials and Tissue Engineering, METU, Ankara, Turkey.

PMID: 25414725 PMCID: PMC4237746 DOI: 10.1186/1678-9199-20-48

Abstract

BACKGROUND: Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs. Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.

METHODS: In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.

RESULTS: Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of 6 to 7 kDa peptides and proved to be a highly practical peptidomics tool with better resolution when compared to conventional polyacrylamide gel electrophoresis. Mass spectroscopy analysis helped us to identify 45 unique peptide masses between 1 to 7 kDa with a bimodal mass distribution peaking between molecular weights of 1 to 2 kDa (29%) and 3 to 4 kDa (31%). L. abdullahbayrami crude venom had a proliferative effect on MCF-7 cells, which may be explained by the high concentration of polyamines as well as potassium and calcium ions in the arachnid venoms. Antimicrobial effect was stronger on gram-negative bacteria.

CONCLUSIONS: This work represents the first peptidomic characterization of L. abdullahbayrami venom. Considering the molecular weight-function relationship of previously identified venom peptides, future bioactivity studies may lead to the discovery of novel potassium and chloride ion channel inhibitors as well as new antimicrobial peptides from L. abdullahbayrami venom.

Keywords: Antimicrobial activity; Cytotoxicity; Electrophoresis; Leiurus abdullahbayrami; Microfluidic capillary; Peptide; Peptidomics; Scorpion venom; Toxin; Turkey; Venomics

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