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Guttikonda VR, Patil R, Kumar G. DNA damage in peripheral blood leukocytes in tobacco users. J Oral Maxillofac Pathol. 2014;18:S16-20doi: 10.4103/0973-029X.141329.
Guttikonda, V. R., Patil, R., & Kumar, G. (2014). DNA damage in peripheral blood leukocytes in tobacco users. Journal of oral and maxillofacial pathology : JOMFP, 18S16-20. https://doi.org/10.4103/0973-029X.141329
Guttikonda, Venkateswara Rao, et al. "DNA damage in peripheral blood leukocytes in tobacco users." Journal of oral and maxillofacial pathology : JOMFP vol. 18 (2014): S16-20. doi: https://doi.org/10.4103/0973-029X.141329
Guttikonda VR, Patil R, Kumar G. DNA damage in peripheral blood leukocytes in tobacco users. J Oral Maxillofac Pathol. 2014 Sep;18:S16-20. doi: 10.4103/0973-029X.141329. PMID: 25364170; PMCID: PMC4211229.
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J Oral Maxillofac Pathol. 2014 Sep;18:S16-20. doi: 10.4103/0973-029X.141329.

DNA damage in peripheral blood leukocytes in tobacco users.

Journal of oral and maxillofacial pathology : JOMFP

Venkateswara Rao Guttikonda, Rekha Patil, Gs Kumar

Affiliations

  1. Department of Oral Pathology, Mamata Dental College and Hospital, Khammam, Andhra Pradesh, India.
  2. Department of Oral Pathology, Raja Rajeswari Dental College and Hospital, Bengaluru, Karnataka, India.
  3. Department of Oral Pathology, K S R institute of Dental Sciences, Tiruchengod, Tamil Nadu, India.

PMID: 25364170 PMCID: PMC4211229 DOI: 10.4103/0973-029X.141329

Abstract

AIM: To Quantify the DNA single-stranded breaks in the peripheral blood leukocytes (PBLs) of tobacco-habituated individuals with clinically normal mucosa and patients with oral carcinoma.

OBJECTIVES: To evaluate DNA damage levels in PBLs of tobacco-habituated individuals with clinically normal mucosa and patients with oral carcinoma and compare with a control group of healthy volunteers. To evaluate the extent of DNA damage in PBLs using Single Cell Gel Electrophoresis (SCGE) in the above groups.

MATERIALS AND METHODS: Patients who were attending the outpatient department were enrolled in this study. A control group of 30 healthy volunteers included in Group I were selected from various age groups who are not tobacco users in any form. Thirty patients with tobacco habituation but with clinically normal mucosa were included in Group II, while 30 tobacco-habituated patients with oral squamous carcinoma were included in Group III. A biopsy was taken from the representative area and confirmed histologically. Intravenous blood samples were collected from all the groups for evaluation of the extent of DNA damage using ethidium bromide-stained slides under fluorescent microscope. The DNA tail length was calculated by subtracting the diameter from the total length. Twenty-five randomly selected cells per slide were analyzed and mean calculated.

RESULTS: The mean DNA damage levels in patients with tobacco habits were compared with that of the control group and the results were found to be statistically significant. The mean DNA damage level in PBLs between tobacco-habituated patients with normal mucosa and oral cancer patients was found to be statistically significant. The DNA damage in cancer patients was compared with the control group and the results were found to be statistically significant.

CONCLUSION: DNA damage evaluation in PBLs by SCGE technique is a sensitive and reliable indicator of tobacco insult.

Keywords: DNA damage; single cell gel electrophoresis (comet assay); tobacco users

References

  1. Mutat Res. 1996 Sep 13;370(2):115-20 - PubMed
  2. Cancer. 1968 Jul;22(1):35-42 - PubMed
  3. Exp Cell Res. 1988 Mar;175(1):184-91 - PubMed
  4. J Oral Pathol Med. 1997 Sep;26(8):377-80 - PubMed
  5. Cancer. 1992 Sep 1;70(5):1017-23 - PubMed
  6. Mutagenesis. 1992 Sep;7(5):357-8 - PubMed
  7. Acta Cytol. 1966 May-Jun;10(3):197-9 - PubMed
  8. Cancer Res. 1993 Apr 1;53(7):1522-8 - PubMed

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