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Ahi EP, Kapralova KH, Pálsson A, et al. Transcriptional dynamics of a conserved gene expression network associated with craniofacial divergence in Arctic charr. Evodevo. 2014;5(1):40doi: 10.1186/2041-9139-5-40.
Ahi, E. P., Kapralova, K. H., Pálsson, A., Maier, V. H., Gudbrandsson, J., Snorrason, S. S., Jónsson, Z. O., & Franzdóttir, S. R. (2014). Transcriptional dynamics of a conserved gene expression network associated with craniofacial divergence in Arctic charr. EvoDevo, 5(1), 40. https://doi.org/10.1186/2041-9139-5-40
Ahi, Ehsan Pashay, et al. "Transcriptional dynamics of a conserved gene expression network associated with craniofacial divergence in Arctic charr." EvoDevo vol. 5,1 (2014): 40. doi: https://doi.org/10.1186/2041-9139-5-40
Ahi EP, Kapralova KH, Pálsson A, Maier VH, Gudbrandsson J, Snorrason SS, Jónsson ZO, Franzdóttir SR. Transcriptional dynamics of a conserved gene expression network associated with craniofacial divergence in Arctic charr. Evodevo. 2014 Nov 03;5(1):40. doi: 10.1186/2041-9139-5-40. eCollection 2014. PMID: 25419450; PMCID: PMC4240837.
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Evodevo. 2014 Nov 03;5(1):40. doi: 10.1186/2041-9139-5-40. eCollection 2014.

Transcriptional dynamics of a conserved gene expression network associated with craniofacial divergence in Arctic charr.

EvoDevo

Ehsan Pashay Ahi, Kalina Hristova Kapralova, Arnar Pálsson, Valerie Helene Maier, Jóhannes Gudbrandsson, Sigurdur S Snorrason, Zophonías O Jónsson, Sigrídur Rut Franzdóttir

Affiliations

  1. Institute of Life and Environmental Sciences, University of Iceland, Sturlugata 7, 101 Reykjavik, Iceland.
  2. Institute of Life and Environmental Sciences, University of Iceland, Sturlugata 7, 101 Reykjavik, Iceland ; Biomedical Center, University of Iceland, Vatnsmýrarvegur 16, 101 Reykjavik, Iceland.

PMID: 25419450 PMCID: PMC4240837 DOI: 10.1186/2041-9139-5-40

Abstract

BACKGROUND: Understanding the molecular basis of craniofacial variation can provide insights into key developmental mechanisms of adaptive changes and their role in trophic divergence and speciation. Arctic charr (Salvelinus alpinus) is a polymorphic fish species, and, in Lake Thingvallavatn in Iceland, four sympatric morphs have evolved distinct craniofacial structures. We conducted a gene expression study on candidates from a conserved gene coexpression network, focusing on the development of craniofacial elements in embryos of two contrasting Arctic charr morphotypes (benthic and limnetic).

RESULTS: Four Arctic charr morphs were studied: one limnetic and two benthic morphs from Lake Thingvallavatn and a limnetic reference aquaculture morph. The presence of morphological differences at developmental stages before the onset of feeding was verified by morphometric analysis. Following up on our previous findings that Mmp2 and Sparc were differentially expressed between morphotypes, we identified a network of genes with conserved coexpression across diverse vertebrate species. A comparative expression study of candidates from this network in developing heads of the four Arctic charr morphs verified the coexpression relationship of these genes and revealed distinct transcriptional dynamics strongly correlated with contrasting craniofacial morphologies (benthic versus limnetic). A literature review and Gene Ontology analysis indicated that a significant proportion of the network genes play a role in extracellular matrix organization and skeletogenesis, and motif enrichment analysis of conserved noncoding regions of network candidates predicted a handful of transcription factors, including Ap1 and Ets2, as potential regulators of the gene network. The expression of Ets2 itself was also found to associate with network gene expression. Genes linked to glucocorticoid signalling were also studied, as both Mmp2 and Sparc are responsive to this pathway. Among those, several transcriptional targets and upstream regulators showed differential expression between the contrasting morphotypes. Interestingly, although selected network genes showed overlapping expression patterns in situ and no morph differences, Timp2 expression patterns differed between morphs.

CONCLUSION: Our comparative study of transcriptional dynamics in divergent craniofacial morphologies of Arctic charr revealed a conserved network of coexpressed genes sharing functional roles in structural morphogenesis. We also implicate transcriptional regulators of the network as targets for future functional studies.

Keywords: Arctic charr; Coexpression; Craniofacial development; Divergent evolution; Gene network; Morphogenesis; Salvelinus alpinus

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