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Fairfoul G, McGuire LI, Pal S, et al. Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies. Ann Clin Transl Neurol. 2016;3(10):812-818doi: 10.1002/acn3.338.
Fairfoul, G., McGuire, L. I., Pal, S., Ironside, J. W., Neumann, J., Christie, S., Joachim, C., Esiri, M., Evetts, S. G., Rolinski, M., Baig, F., Ruffmann, C., Wade-Martins, R., Hu, M. T., Parkkinen, L., & Green, A. J. (2016). Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies. Annals of clinical and translational neurology, 3(10), 812-818. https://doi.org/10.1002/acn3.338
Fairfoul, Graham, et al. "Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies." Annals of clinical and translational neurology vol. 3,10 (2016): 812-818. doi: https://doi.org/10.1002/acn3.338
Fairfoul G, McGuire LI, Pal S, Ironside JW, Neumann J, Christie S, Joachim C, Esiri M, Evetts SG, Rolinski M, Baig F, Ruffmann C, Wade-Martins R, Hu MT, Parkkinen L, Green AJ. Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies. Ann Clin Transl Neurol. 2016 Aug 28;3(10):812-818. doi: 10.1002/acn3.338. eCollection 2016 Oct. PMID: 27752516; PMCID: PMC5048391.
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Ann Clin Transl Neurol. 2016 Aug 28;3(10):812-818. doi: 10.1002/acn3.338. eCollection 2016 Oct.

Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies.

Annals of clinical and translational neurology

Graham Fairfoul, Lynne I McGuire, Suvankar Pal, James W Ironside, Juliane Neumann, Sharon Christie, Catherine Joachim, Margaret Esiri, Samuel G Evetts, Michal Rolinski, Fahd Baig, Claudio Ruffmann, Richard Wade-Martins, Michele T M Hu, Laura Parkkinen, Alison J E Green

Affiliations

  1. The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom.
  2. The National CJD Research & Surveillance Unit Western General Hospital University of Edinburgh Edinburgh EH4 2XU United Kingdom; Centre for Clinical Brain Sciences Western General Hospital Edinburgh EH4 2XU United Kingdom.
  3. Oxford Parkinson's Disease Centre Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
  4. Oxford Project to Investigate Memory and Ageing (OPTIMA) Nuffield Department of Clinical Neurosciences University of Oxford John Radcliffe Hospital Level 6 West Wing Oxford OX3 9DU United Kingdom.
  5. Oxford Parkinson's Disease Centre Department of Physiology Anatomy and Genetics University of Oxford Le Gros Clark Building, South Parks Road Oxford OX1 3QX United Kingdom.

PMID: 27752516 PMCID: PMC5048391 DOI: 10.1002/acn3.338

Abstract

We have developed a novel real-time quaking-induced conversion RT-QuIC-based assay to detect alpha-synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha-synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT-QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha-synucleinopathies.

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