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Kesterson RA, Johnson LW, Lambert LJ, et al. Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer. Bio Protoc. 2016;6(20)doi: 10.21769/BioProtoc.1976.
Kesterson, R. A., Johnson, L. W., Lambert, L. J., Vivian, J. L., Welch, D. R., & Ballinger, S. W. (2016). Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer. Bio-protocol, 6(20), . https://doi.org/10.21769/BioProtoc.1976
Kesterson, Robert A, et al. "Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer." Bio-protocol vol. 6,20 (2016). doi: https://doi.org/10.21769/BioProtoc.1976
Kesterson RA, Johnson LW, Lambert LJ, Vivian JL, Welch DR, Ballinger SW. Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer. Bio Protoc. 2016 Oct 20;6(20). doi: 10.21769/BioProtoc.1976. PMID: 27840835; PMCID: PMC5100744.
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Bio Protoc. 2016 Oct 20;6(20). doi: 10.21769/BioProtoc.1976.

Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer.

Bio-protocol

Robert A Kesterson, Larry W Johnson, Laura J Lambert, Jay L Vivian, Danny R Welch, Scott W Ballinger

Affiliations

  1. Department of Genetics, University of Alabama, Birmingham, USA.
  2. Department of Pathology, University of Kansas Medical Center, Kansas City, USA.
  3. Department of Cancer Biology, University of Kansas Cancer Center, Kansas City, USA.
  4. Division of Molecular and Cellular Pathology, University of Alabama, Birmingham, USA.

PMID: 27840835 PMCID: PMC5100744 DOI: 10.21769/BioProtoc.1976

Abstract

The mitochondrial paradigm for common disease proposes that mitochondrial DNA (mtDNA) sequence variation can contribute to disease susceptibility and progression. To test this concept, we developed the Mitochondrial-nuclear eXchange (MNX) model, in which isolated embryonic pronuclei from one strain of species are implanted into an enucleated embryo of a different strain of the same species (

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