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Ghieth MA, Kotb MA, Abu-Sarea EY, et al. Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals. J Parasit Dis. 2015;40(4):1470-1474doi: 10.1007/s12639-015-0714-9.
Ghieth, M. A., Kotb, M. A., Abu-Sarea, E. Y., & El-Badry, A. A. (2016). Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals. Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 40(4), 1470-1474. https://doi.org/10.1007/s12639-015-0714-9
Ghieth, Marwa A, et al. "Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals." Journal of parasitic diseases : official organ of the Indian Society for Parasitology vol. 40,4 (2016): 1470-1474. doi: https://doi.org/10.1007/s12639-015-0714-9
Ghieth MA, Kotb MA, Abu-Sarea EY, El-Badry AA. Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals. J Parasit Dis. 2016 Dec;40(4):1470-1474. doi: 10.1007/s12639-015-0714-9. Epub 2015 Sep 18. PMID: 27876969; PMCID: PMC5118340.
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J Parasit Dis. 2016 Dec;40(4):1470-1474. doi: 10.1007/s12639-015-0714-9. Epub 2015 Sep 18.

Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals.

Journal of parasitic diseases : official organ of the Indian Society for Parasitology

Marwa A Ghieth, Magd A Kotb, Enas Y Abu-Sarea, Ayman A El-Badry

Affiliations

  1. Department of Medical Parasitology, Faculty of Medicine, Beni Suef University, Beni Suef, Egypt.
  2. Department of Pediatrics, Kasr Al-Ainy Faculty of Medicine, Cairo University, Giza, Egypt.
  3. Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Giza, Egypt.

PMID: 27876969 PMCID: PMC5118340 DOI: 10.1007/s12639-015-0714-9

Abstract

Giardiasis is a prevailing intestinal disease in children. This study aimed to determine molecular prevalence of Giardia intestinalis in children attending Cairo University Pediatrics Hospitals, using copro-PCR assays, conventional methods and to evaluate diagnostic effectiveness of used tests. 229 fecal samples were collected from children suffering from gastrointestinal symptoms and examined for Giardia by microscopy, Immuno-chromatographic test (ICT), copro-DNA using two PCR assays targeting tpi [nested-PCR (nPCR)] and 18S [conventional-PCR (cPCR)] genes. Out of 229 samples assessed, Giardia was diagnosed in 13.9, 17, 17.9, 4.8 % of cases using microscopy, ICT, nPCR (tpi) and cPCR (18S), respectively. Nominating both PCR assays as composite reference standard, microscopy and ICT were of reliable specificity (100 and 96.9 %) and accuracy (95.6 and 93.6 %) but of limited sensitivity (78.6 and 76.2 %). Kappa agreement showed, there was substantial agreement of ICT (0.776) and almost perfect agreement of microscopy (0.839) with PCR assays.

Keywords: Copro-DNA; Giardia intestinalis; Molecular prevalence; PCR

Conflict of interest statement

The authors declare that they have no reported conflicts of interest. Author contribution All manuscript authors contributed to every activity of it; idea of paper, study design, collection of materia

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