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Hart RA, Dobos RC, Agnew LL, et al. Leptin pharmacokinetics in male mice. Endocr Connect. 2016;6(1):20-26doi: 10.1530/EC-16-0089.
Hart, R. A., Dobos, R. C., Agnew, L. L., Smart, N. A., & McFarlane, J. R. (2017). Leptin pharmacokinetics in male mice. Endocrine connections, 6(1), 20-26. https://doi.org/10.1530/EC-16-0089
Hart, Robert A, et al. "Leptin pharmacokinetics in male mice." Endocrine connections vol. 6,1 (2017): 20-26. doi: https://doi.org/10.1530/EC-16-0089
Hart RA, Dobos RC, Agnew LL, Smart NA, McFarlane JR. Leptin pharmacokinetics in male mice. Endocr Connect. 2017 Jan;6(1):20-26. doi: 10.1530/EC-16-0089. Epub 2016 Dec 20. PMID: 27998953; PMCID: PMC5302164.
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Endocr Connect. 2017 Jan;6(1):20-26. doi: 10.1530/EC-16-0089. Epub 2016 Dec 20.

Leptin pharmacokinetics in male mice.

Endocrine connections

Robert A Hart, Robin C Dobos, Linda L Agnew, Neil A Smart, James R McFarlane

Affiliations

  1. Centre for Bioactive Discovery in Health and AgeingUniversity of New England, Armidale, New South Wales, Australia [email protected].
  2. NSW Department of Primary IndustriesArmidale, New South Wales, Australia.
  3. Centre for Bioactive Discovery in Health and AgeingUniversity of New England, Armidale, New South Wales, Australia.

PMID: 27998953 PMCID: PMC5302164 DOI: 10.1530/EC-16-0089

Abstract

Pharmacokinetics of leptin in mammals has not been studied in detail and only one study has examined more than one time point in non-mutant mice and this was in a female mice. This is the first study to describe leptin distribution over a detailed time course in normal male mice. A physiologic dose (12 ng) of radiolabelled leptin was injected into adult male mice via the lateral tail vein and tissues were dissected out and measured for radioactivity over a time course of up to two hours. Major targets were the digestive tract, kidneys, skin and lungs. The brain was not a major target, and 0.15% of the total dose was recovered from the brain 5 min after administration. Major differences appear to exist in the distribution of leptin between the male and female mice, indicating a high degree of sexual dimorphism. Although the half-lives were similar between male and female mice, almost twice the proportion of leptin was recovered from the digestive tract of male mice in comparison to that reported previously for females. This would seem to indicate a major difference in leptin distribution and possibly function between males and females.

© 2017 The authors.

Keywords: dimorphism; gastrointestinal tract; leptin; mice; pharmacokinetics

References

  1. Neuron. 1999 Aug;23 (4):775-86 - PubMed
  2. Science. 1995 Jul 28;269(5223):543-6 - PubMed
  3. Nature. 1994 Dec 1;372(6505):425-32 - PubMed
  4. Endocrinology. 2001 Dec;142(12):5198-202 - PubMed
  5. Nat Genet. 1996 Sep;14(1):95-7 - PubMed
  6. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6231-5 - PubMed
  7. Endocrinology. 1997 Feb;138(2):855-8 - PubMed
  8. Gut. 2000 Aug;47(2):178-83 - PubMed
  9. Physiol Res. 2016 Jun 20;65(2):311-20 - PubMed
  10. Am J Physiol. 1997 Dec;273(6 Pt 1):E1102-6 - PubMed
  11. J Clin Endocrinol Metab. 1963 Dec;23:1285-97 - PubMed
  12. J Pharmacol Exp Ther. 2003 Apr;305(1):362-7 - PubMed
  13. J Clin Endocrinol Metab. 1996 Sep;81(9):3424-7 - PubMed
  14. N Engl J Med. 1996 Feb 1;334(5):292-5 - PubMed
  15. Am J Physiol. 1997 Nov;273(5 Pt 1):E903-7 - PubMed
  16. Gastroenterology. 2002 Feb;122(2):259-63 - PubMed
  17. Clin Chem. 1998 Mar;44(3):565-70 - PubMed
  18. J Clin Invest. 2001 Nov;108(10):1483-94 - PubMed
  19. Nature. 1995 Oct 12;377(6549):530-2 - PubMed
  20. J Clin Invest. 2011 Mar;121(3):1191-8 - PubMed
  21. Gut. 2002 Jun;50(6):797-802 - PubMed
  22. J Endocrinol. 2006 Jul;190(1):117-27 - PubMed
  23. J Clin Endocrinol Metab. 1996 Nov;81(11):3909-13 - PubMed
  24. Curr Biol. 1996 Sep 1;6(9):1170-80 - PubMed
  25. J Endocrinol. 2003 May;177(2):215-22 - PubMed
  26. J Clin Endocrinol Metab. 1999 Jul;84(7):2270-4 - PubMed
  27. J Endocrinol. 1999 May;161(2):211-8 - PubMed
  28. Science. 1966 Sep 2;153(3740):1127-8 - PubMed
  29. Mol Reprod Dev. 2008 May;75(5):874-80 - PubMed
  30. FEBS Lett. 1998 Feb 27;423(3):302-6 - PubMed
  31. J Physiol. 1973 Jan;228(2):279-84 - PubMed
  32. Nat Med. 1995 Dec;1(12):1311-4 - PubMed
  33. Horm Metab Res. 1996 Dec;28(12):653-8 - PubMed
  34. Int J Obes Relat Metab Disord. 2000 Dec;24(12):1579-85 - PubMed
  35. FASEB J. 1998 Jan;12(1):57-65 - PubMed
  36. J Reprod Immunol. 2012 Dec;96(1-2):19-24 - PubMed
  37. Nature. 1996 Feb 15;379(6566):632-5 - PubMed
  38. Comp Biochem Physiol A Mol Integr Physiol. 2004 May;138(1):27-32 - PubMed
  39. Pediatr Res. 2006 Aug;60(2):200-4 - PubMed
  40. Proc Nutr Soc. 1998 Aug;57(3):441-8 - PubMed
  41. FEBS Lett. 2002 Mar 27;515(1-3):109-13 - PubMed
  42. Nat Med. 1997 Sep;3(9):1029-33 - PubMed
  43. Am J Physiol Regul Integr Comp Physiol. 2005 Apr;288(4):R879-84 - PubMed
  44. Int J Obes Relat Metab Disord. 1998 Aug;22(8):765-70 - PubMed
  45. J Hered. 1950 Dec;41(12):317-8 - PubMed
  46. Diabetologia. 1997 Oct;40(10):1178-84 - PubMed
  47. Nature. 1998 Oct 22;395(6704):763-70 - PubMed
  48. Int J Obes Relat Metab Disord. 1996 Dec;20(12):1120-6 - PubMed
  49. Obes Res. 2004 Sep;12(9):1481-91 - PubMed
  50. Endocrinology. 1997 Mar;138(3):1190-3 - PubMed
  51. Diabetes. 1996 Jul;45(7):984-7 - PubMed
  52. J Clin Endocrinol Metab. 1997 Feb;82(2):579-84 - PubMed
  53. Nature. 1998 Aug 20;394(6695):790-3 - PubMed
  54. Proc Soc Exp Biol Med. 1950 Oct;75(1):16-20 - PubMed
  55. Anat Rec. 1973 Nov;177(3):465-9 - PubMed
  56. J Physiol Biochem. 2001 Dec;57(4):345-6 - PubMed
  57. Mucosal Immunol. 2011 May;4(3):294-303 - PubMed
  58. Diabetes. 2001 Feb;50(2):425-35 - PubMed
  59. Endocrinology. 1999 Apr;140(4):1630-8 - PubMed
  60. Diabetes. 1997 Aug;46(8):1360-3 - PubMed

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