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RA LaCroix, TE Sandberg, EJ O'Brien… - Applied and …, 2015 - Am Soc Microbiol

Use of adaptive laboratory evolution to discover key mutations enabling rapid growth of Escherichia coli K-12 MG1655 on glucose minimal medium.

k-12

Sandberg, O'Brien

GSID: R9eLUhzErdsJ

Excerpt

… coli K-12 MG1655 was adaptively evolved in minimal medium at 37C with excess glucose in eight parallel experiments. At the end of the ALE experiments, clones from the final populations were characterized in terms of their growth rate, metabolic uptake and secretion rates, …

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