A Oka, K Sugimoto, M Takanami, Y Hirota - Molecular and General …, 1980 - Springer

Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication.

k-12

Oka

GSID: 2jz8tLLz9nwJ

Excerpt

A DNA fragment containing the replication origin of the Escherichia coli K-12 chromosome was inserted in two orientations at either the BamHI or SalI site of pBR322 DNA. All the resulting hybrid plasmids were found to replicate in both polA and polA + cells, whereas pBR322 …

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