Home
About Us
UI Blog
Contact Us
Clipboard & History
Search history (0)
Clipboard (0)
searchable interface
Affiliation
All Fields
Author
Author - First
Author - Identifier
Author - Last
Book
Conflict of Interest Statements
Editor
Issue
Journal
Language
MeSH Terms
Pagination
Publication Type
Publication Year
Publisher
Title
Title/Abstract
Transliterated Title
Volume
Find
Please fill out this field.
Display options
Format
Abstract
PubMed
PMID
Save
Email
Cite
Cite
AMA
Collaborative Research: Characterizing the Constraints on Virus Infection of Cyanobacteria. ;
APA
(). Collaborative Research: Characterizing the Constraints on Virus Infection of Cyanobacteria. .
MLA
"Collaborative Research: Characterizing the Constraints on Virus Infection of Cyanobacteria." vol. ().
NLM
Collaborative Research: Characterizing the Constraints on Virus Infection of Cyanobacteria. UIID-NSF: 395.
Copy
Download .nbib
Format:
NLM
AMA
APA
MLA
NLM
Send to
Clipboard
My Bibliography
Collections
Citation Manager
Share it on
Link
Direct link
Direct link
Collaborative Research: Characterizing the Constraints on Virus Infection of Cyanobacteria.
[No authors listed]
UIID-NSF: 395
Abstract
"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)." It is well established that viruses play an important role in regulating the structure and function of marine ecosystems. However, we lack a mechanistic understanding of how bacteria cell receptors constrain virus infection, which has implications for the ecology and evolutionary dynamics of marine microbial food webs. This project will identify receptors for three known groups of cyanophage (podo-, myo- and siphoviridae) on the on the outer membrane of the marine cyanobacterium Synechococcus sp. WH7803. The researchers will employ a series of proven methods to generate virus-resistant Synechococcus populations using chemostats, to narrow the mutants using fluorescent virus tags that monitor surface attachment, and to characterize the outer membrane protein compliment of the resistant populations using LC-MS/MS proteomic analyses. Once the receptors are identified, bioinformatic analyses of existing databases will be coupled with molecular biological queries of sample archives to document the diversity and prevalence of the different receptors in marine cyanobacterial genomes as well as of those expressed by natural populations. Lab studies in chemostats swill examine the regulation of receptor expression (using fluorescence viruses and qRT-PCR for gene expression) so as to begin to tease apart the environmental controls on virus receptor expression in culture populations of cyanobacteria. All information will be collected in a manner that will make it suitable for analyses by ecological competition models, thereby providing new insights on the cost-of-resistance to infection that cyanobacteria face as well as new information on how virus infection of cells may be constrained by environmental conditions. Broader Impacts: The broader impacts of this project include the training of a new generation of marine scientists in interdisciplinary sciences of microbiology, molecular biology and proteomics, marine geochemistry and classical ecology of students at an EPSCoR Institution (The University of Tennessee) and a research station (Kellogg Biological Station). The study will provide opportunities for students to work with researchers from a major federal research facility (Oak Ridge National Laboratory) where access to equipment exists that is not available at most academic institutions. Information from this study will be directly incorporated into course work, educational and public outreach, and disseminated through a novel partnership with the Science Journalism program at the University of Tennessee. One or more undergraduates interested in science journalism will be recruited in order to generate materials for paper and electronic dissemination on marine microbiology and ecology that will be appropriate for K-12 students as well as the general public. The PIs will continue their well-established history of recruitment of underrepresented groups and participation in methods-oriented courses. Scientifically the information generated by this study will be completely novel, extending and transforming the foundations of marine microbiology and virus ecology as well as providing insight into how shifts in the global climate and ocean biogeochemistry might influence microbial proliferation and control by marine viruses.
Other Details
Award Instrument:
Standard Grant
Email:
[email protected]
Organization:
University of Tennessee Knoxville
Other Investigators:
Michelle Sauther, Nathan VerBerkmoes, Nayuta Yamashita
Primary Investigator:
Steven Wilhelm
Program(s):
BIOLOGICAL OCEANOGRAPHY
Start Date:
06/15/2009
Save results to a file
No records selected. Please select records to continue.
Format
Summary (text)
PubMed
PMID
Abstract (text)
CSV
Email results
Only first 240 records will be saved in your file.
No records selected. Please select records to continue.
Email subject
UIINDEX - UIID-NSF: 395
Send email to
Format
Summary
Summary (text)
Abstract
Abstract (text)
Captcha
Citation copied successfully.