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Yan, W. X., Hunnewell, P., Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., ... & Scott, D. A. (2019). Functionally diverse type V CRISPR-Cas systems. Science, 363(6422), 88-91.
Yan, Winston X., et al. "Functionally diverse type V CRISPR-Cas systems." Science 363.6422 (2019): 88-91.
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WX Yan, P Hunnewell, LE Alfonse, JM Carte… - Science, 2019 - science.org

Functionally diverse type V CRISPR-Cas systems.

Computer and Information Science and Engineering Minority-Serving Institutions Research Expansion Program AND Program Evaluation

Yan

GSID: -YZqT-35eywJ

Excerpt

Type V CRISPR-Cas systems are distinguished by a single RNA-guided RuvC domain-containing effector, Cas12. Although effectors of subtypes VA (Cas12a) and VB (Cas12b) have been studied in detail, the distinct domain architectures and diverged RuvC sequences of uncharacterized Cas12 proteins suggest unexplored functional diversity. Here, we identify and characterize Cas12c,-g,-h, and-i. Cas12c,-h, and-i demonstrate RNA-guided double-stranded DNA (dsDNA) interference activity. Cas12i exhibits markedly different efficiencies …

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