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Tattersall, E. A., Ergul, A., AlKayal, F., DeLuc, L., Cushman, J. C., & Cramer, G. R. (2005). Comparison of methods for isolating high-quality RNA from leaves of grapevine. American Journal of Enology and Viticulture, 56(4), 400-406.
Tattersall, Elizabeth AR, et al. "Comparison of methods for isolating high-quality RNA from leaves of grapevine." American Journal of Enology and Viticulture 56.4 (2005): 400-406.
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EAR Tattersall, A Ergul, F AlKayal… - … Journal of Enology …, 2005 - Am Soc Enol Viticulture

Comparison of methods for isolating high-quality RNA from leaves of grapevine.

EPSCoR Research Infrastructure Improvement Program AND Program Evaluation

AlKayal, Ergul

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Excerpt

High concentrations of polyphenols and polysaccharides make it challenging to extract high-quality RNA from grape organs. To determine an optimal protocol for grape leaves, 15 different methods of RNA extraction were evaluated based on cost, time to complete the extraction, and quality of the RNA isolated. The addition of specific compounds to the extraction buffer to remove polyphenols and polysaccharides is often critical for downstream applications such as polymerase chain reaction (PCR) and microarray hybridization. RNA …

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