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Ream, J. A., Lewis, L. K., & Lewis, K. A. (2016). Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions. Analytical biochemistry, 511, 36-41.
Ream, Jennifer A., L. Kevin Lewis, and Karen A. Lewis. "Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions." Analytical biochemistry 511 (2016): 36-41.
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JA Ream, LK Lewis, KA Lewis - Analytical biochemistry, 2016 - Elsevier

Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions.

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Interactions between proteins and nucleic acids are frequently analyzed using electrophoretic mobility shift assays (EMSAs). This technique separates bound protein: nucleic acid complexes from free nucleic acids by electrophoresis, most commonly using polyacrylamide gels. The current study utilizes recent advances in agarose gel electrophoresis technology to develop a new EMSA protocol that is simpler and faster than traditional polyacrylamide methods. Agarose gels are normally run at low voltages (∼ 10 …

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