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Cornish-Bowden A, Eisenthal R. Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods. Biochem J. 1974;139(3):721-30doi: 10.1042/bj1390721.
Cornish-Bowden, A., & Eisenthal, R. (1974). Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods. The Biochemical journal, 139(3), 721-30. https://doi.org/10.1042/bj1390721
Cornish-Bowden, A, and Eisenthal, R. "Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods." The Biochemical journal vol. 139,3 (1974): 721-30. doi: https://doi.org/10.1042/bj1390721
Cornish-Bowden A, Eisenthal R. Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods. Biochem J. 1974 Jun;139(3):721-30. doi: 10.1042/bj1390721. PMID: 4854389; PMCID: PMC1166336.
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Biochem J. 1974 Jun;139(3):721-30. doi: 10.1042/bj1390721.

Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods.

The Biochemical journal

A Cornish-Bowden, R Eisenthal

PMID: 4854389 PMCID: PMC1166336 DOI: 10.1042/bj1390721
Free PMC Article

Abstract

The statistical implications of the direct linear plot for enzyme kinetic data, described in the preceding paper (Eisenthal & Cornish-Bowden, 1974), are discussed for the case of the Michaelis-Menten equation. The plot is shown to lead directly to non-parametric confidence limits for the kinetic parameters, V and K(m), which depend on far less sweeping assumptions about the nature of experimental error than those implicit in the method of least squares. Median estimates of V and K(m) can also be defined, which are shown to be more robust than the least-squares estimates in a wide variety of experimental situations.

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