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Engasser JM, Hisland P. Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions. Biochem J. 1978;173(1):341-3doi: 10.1042/bj1730341.
Engasser, J. M., & Hisland, P. (1978). Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions. The Biochemical journal, 173(1), 341-3. https://doi.org/10.1042/bj1730341
Engasser, J M, and Hisland, P. "Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions." The Biochemical journal vol. 173,1 (1978): 341-3. doi: https://doi.org/10.1042/bj1730341
Engasser JM, Hisland P. Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions. Biochem J. 1978 Jul 01;173(1):341-3. doi: 10.1042/bj1730341. PMID: 687375; PMCID: PMC1185780.
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Biochem J. 1978 Jul 01;173(1):341-3. doi: 10.1042/bj1730341.

Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions.

The Biochemical journal

J M Engasser, P Hisland

PMID: 687375 PMCID: PMC1185780 DOI: 10.1042/bj1730341
Free PMC Article

Abstract

Diffusional effects on two-substrate enzymic reactions mainly depend on the relative affinities of the enzyme for its two substrates. With two substrates of widely different affinities, diffusional limitations increase and decrease the half-maximal-activity concentration of the high-and low-affinity substrate respectively.

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