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Kirkland JJ, Yau WW, Stoklosa HJ, et al. Sampling and extra-column effects in high-performance liquid chromatography; influence of peak skew on plate count calculations. J Chromatogr Sci. 1977;15(8):303-16doi: 10.1093/chromsci/15.8.303.
Kirkland, J. J., Yau, W. W., Stoklosa, H. J., & Dilks, C. H. (1977). Sampling and extra-column effects in high-performance liquid chromatography; influence of peak skew on plate count calculations. Journal of chromatographic science, 15(8), 303-16. https://doi.org/10.1093/chromsci/15.8.303
Kirkland, J J, et al. "Sampling and extra-column effects in high-performance liquid chromatography; influence of peak skew on plate count calculations." Journal of chromatographic science vol. 15,8 (1977): 303-16. doi: https://doi.org/10.1093/chromsci/15.8.303
Kirkland JJ, Yau WW, Stoklosa HJ, Dilks CH. Sampling and extra-column effects in high-performance liquid chromatography; influence of peak skew on plate count calculations. J Chromatogr Sci. 1977 Aug;15(8):303-16. doi: 10.1093/chromsci/15.8.303. PMID: 893648.
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J Chromatogr Sci. 1977 Aug;15(8):303-16. doi: 10.1093/chromsci/15.8.303.

Sampling and extra-column effects in high-performance liquid chromatography; influence of peak skew on plate count calculations.

Journal of chromatographic science

J J Kirkland, W W Yau, H J Stoklosa, C H Dilks

PMID: 893648 DOI: 10.1093/chromsci/15.8.303

Abstract

Chromatographic resolution and analysis accuracy can be seriously affected by broadening of very sharp bands associated with both high-performance size-exclusion chromatography and other liquid chromatography methods using less than 5 micron particles. The effect on column plate count by extra-column band broadening from injection systems, detectors, connectors and "guard" columns is discussed and exemplified with experimental data. The special cases of tailing and doublet peaks for a single species are investigated. These effects are eliminated by a special sample injection technique. Computer simulation studies demonstrated that serious errors can result from calculating column plate count with non-Gaussian peaks. The maximum allowable peak tailing is suggested and a new method for quantitating peak skew is proposed.

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