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Lab techniques for a more sustainable world.

BioTechniques

Chard L.
PMID: 34587814
Biotechniques. 2021 Oct;71(4):501-504. doi: 10.2144/btn-2021-0081. Epub 2021 Sep 30.

Sustainability is an incredibly important topic for people to consider at the moment. Find out how your lab can make changes to reach a more sustainable future in our latest Tech News.

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Chard L. Lab techniques for a more sustainable world. Biotechniques. 2021;71(4):501-504doi: 10.2144/btn-2021-0081.
Chard, L. (2021). Lab techniques for a more sustainable world. BioTechniques, 71(4), 501-504. https://doi.org/10.2144/btn-2021-0081
Chard, Lucy. "Lab techniques for a more sustainable world." BioTechniques vol. 71,4 (2021): 501-504. doi: https://doi.org/10.2144/btn-2021-0081
Chard L. Lab techniques for a more sustainable world. Biotechniques. 2021 Oct;71(4):501-504. doi: 10.2144/btn-2021-0081. Epub 2021 Sep 30. PMID: 34587814.
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Assay system for mesocotyl elongation and hydrotropism of maize primary root in response to low moisture gradient.

BioTechniques

Sáenz-Rodríguez MN, Cassab López GI.
PMID: 34617460
Biotechniques. 2021 Oct;71(4):516-527. doi: 10.2144/btn-2021-0034. Epub 2021 Oct 07.

We designed and validated a test system that simulates a growth environment for 

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Sáenz-Rodríguez MN, Cassab López GI. Assay system for mesocotyl elongation and hydrotropism of maize primary root in response to low moisture gradient. Biotechniques. 2021;71(4):516-527doi: 10.2144/btn-2021-0034.
Sáenz-Rodríguez, M. N., & Cassab López, G. I. (2021). Assay system for mesocotyl elongation and hydrotropism of maize primary root in response to low moisture gradient. BioTechniques, 71(4), 516-527. https://doi.org/10.2144/btn-2021-0034
Sáenz-Rodríguez, Mery Nair, and Cassab López, Gladys Iliana. "Assay system for mesocotyl elongation and hydrotropism of maize primary root in response to low moisture gradient." BioTechniques vol. 71,4 (2021): 516-527. doi: https://doi.org/10.2144/btn-2021-0034
Sáenz-Rodríguez MN, Cassab López GI. Assay system for mesocotyl elongation and hydrotropism of maize primary root in response to low moisture gradient. Biotechniques. 2021 Oct;71(4):516-527. doi: 10.2144/btn-2021-0034. Epub 2021 Oct 07. PMID: 34617460.
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A short interspersed nuclear element-based quantitative PCR assay for simultaneous human and dog DNA detection and quantification.

BioTechniques

Liang JW, Coyle HM.
PMID: 33307787
Biotechniques. 2021 Mar;70(3):175-180. doi: 10.2144/btn-2020-0144. Epub 2020 Dec 14.

This study aimed to develop a quantitative PCR assay to simultaneously quantify human and dog DNA in a multispecies mixture to inform downstream analyses in routine forensic casework and scientific research. The human target is the

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Liang JW, Coyle HM. A short interspersed nuclear element-based quantitative PCR assay for simultaneous human and dog DNA detection and quantification. Biotechniques. 2020;70(3):175-180doi: 10.2144/btn-2020-0144.
Liang, J. W., & Coyle, H. M. (2021). A short interspersed nuclear element-based quantitative PCR assay for simultaneous human and dog DNA detection and quantification. BioTechniques, 70(3), 175-180. https://doi.org/10.2144/btn-2020-0144
Liang, James W, and Coyle, Heather Miller. "A short interspersed nuclear element-based quantitative PCR assay for simultaneous human and dog DNA detection and quantification." BioTechniques vol. 70,3 (2021): 175-180. doi: https://doi.org/10.2144/btn-2020-0144
Liang JW, Coyle HM. A short interspersed nuclear element-based quantitative PCR assay for simultaneous human and dog DNA detection and quantification. Biotechniques. 2021 Mar;70(3):175-180. doi: 10.2144/btn-2020-0144. Epub 2020 Dec 14. PMID: 33307787.
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Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering.

BioTechniques

Hsieh DJ, Srinivasan P, Yen KC, Yeh YC, Chen YJ, Wang HC, Tarng YW.
PMID: 33307815
Biotechniques. 2021 Feb;70(2):107-115. doi: 10.2144/btn-2020-0141. Epub 2020 Dec 14.

Extracellular matrix (ECM) scaffolds are extensively used in tissue engineering studies and numerous clinical applications for tissue and organ reconstructions. Due to the global severe shortage of human tissues and organs, xenogeneic biomaterials are a common source for human...

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Hsieh DJ, Srinivasan P, Yen KC, et al. Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering. Biotechniques. 2020;70(2):107-115doi: 10.2144/btn-2020-0141.
Hsieh, D. J., Srinivasan, P., Yen, K. C., Yeh, Y. C., Chen, Y. J., Wang, H. C., & Tarng, Y. W. (2021). Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering. BioTechniques, 70(2), 107-115. https://doi.org/10.2144/btn-2020-0141
Hsieh, Dar-Jen, et al. "Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering." BioTechniques vol. 70,2 (2021): 107-115. doi: https://doi.org/10.2144/btn-2020-0141
Hsieh DJ, Srinivasan P, Yen KC, Yeh YC, Chen YJ, Wang HC, Tarng YW. Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering. Biotechniques. 2021 Feb;70(2):107-115. doi: 10.2144/btn-2020-0141. Epub 2020 Dec 14. PMID: 33307815.
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A method for the temperature-controlled extraction of DNA from ancient bones.

BioTechniques

Essel E, Korlević P, Meyer M.
PMID: 34164993
Biotechniques. 2021 Jul;71(1):382-386. doi: 10.2144/btn-2021-0025. Epub 2021 Jun 24.

Contamination with microbial and other exogenous DNA poses a significant challenge in the generation of genome-wide sequence data from ancient skeletal remains. Here we describe a method for separating ancient DNA into multiple fractions during DNA extraction by sequential...

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Essel E, Korlević P, Meyer M. A method for the temperature-controlled extraction of DNA from ancient bones. Biotechniques. 2021;71(1):382-386doi: 10.2144/btn-2021-0025.
Essel, E., Korlević, P., & Meyer, M. (2021). A method for the temperature-controlled extraction of DNA from ancient bones. BioTechniques, 71(1), 382-386. https://doi.org/10.2144/btn-2021-0025
Essel, Elena, et al. "A method for the temperature-controlled extraction of DNA from ancient bones." BioTechniques vol. 71,1 (2021): 382-386. doi: https://doi.org/10.2144/btn-2021-0025
Essel E, Korlević P, Meyer M. A method for the temperature-controlled extraction of DNA from ancient bones. Biotechniques. 2021 Jul;71(1):382-386. doi: 10.2144/btn-2021-0025. Epub 2021 Jun 24. PMID: 34164993.
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Colonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis.

BioTechniques

Ahmad R, Kumar B, Tamang RL, Xu W, Talmon GA, Mohs AM, Dhawan P, Singh AB.
PMID: 34392706
Biotechniques. 2021 Sep;71(3):456-464. doi: 10.2144/btn-2020-0172. Epub 2021 Aug 16.

The conventional orthotopic/xenograft models or genetically engineered murine models of colon cancer (CRC) are limited in their scope for a true understanding of tumor growth, progression and eventual metastasis in its natural microenvironment. In the currently used murine models...

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Ahmad R, Kumar B, Tamang RL, et al. Colonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis. Biotechniques. 2021;71(3):456-464doi: 10.2144/btn-2020-0172.
Ahmad, R., Kumar, B., Tamang, R. L., Xu, W., Talmon, G. A., Mohs, A. M., Dhawan, P., & Singh, A. B. (2021). Colonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis. BioTechniques, 71(3), 456-464. https://doi.org/10.2144/btn-2020-0172
Ahmad, Rizwan, et al. "Colonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis." BioTechniques vol. 71,3 (2021): 456-464. doi: https://doi.org/10.2144/btn-2020-0172
Ahmad R, Kumar B, Tamang RL, Xu W, Talmon GA, Mohs AM, Dhawan P, Singh AB. Colonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis. Biotechniques. 2021 Sep;71(3):456-464. doi: 10.2144/btn-2020-0172. Epub 2021 Aug 16. PMID: 34392706.
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Design and characterization of a 3D-printed staggered herringbone mixer.

BioTechniques

Shenoy VJ, Edwards CE, Helgeson ME, Valentine MT.
PMID: 34000813
Biotechniques. 2021 May;70(5):285-289. doi: 10.2144/btn-2021-0009. Epub 2021 May 18.

3D printing holds potential as a faster, cheaper alternative compared with traditional photolithography for the fabrication of microfluidic devices by replica molding. However, the influence of printing resolution and quality on device design and performance has yet to receive...

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Shenoy VJ, Edwards CE, Helgeson ME, et al. Design and characterization of a 3D-printed staggered herringbone mixer. Biotechniques. 2021;70(5):285-289doi: 10.2144/btn-2021-0009.
Shenoy, V. J., Edwards, C. E., Helgeson, M. E., & Valentine, M. T. (2021). Design and characterization of a 3D-printed staggered herringbone mixer. BioTechniques, 70(5), 285-289. https://doi.org/10.2144/btn-2021-0009
Shenoy, Vedika J, et al. "Design and characterization of a 3D-printed staggered herringbone mixer." BioTechniques vol. 70,5 (2021): 285-289. doi: https://doi.org/10.2144/btn-2021-0009
Shenoy VJ, Edwards CE, Helgeson ME, Valentine MT. Design and characterization of a 3D-printed staggered herringbone mixer. Biotechniques. 2021 May;70(5):285-289. doi: 10.2144/btn-2021-0009. Epub 2021 May 18. PMID: 34000813.
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Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells.

BioTechniques

Xu T, Gilliam M, Sayler G, Ripp S, Close D.
PMID: 34350768
Biotechniques. 2021 Aug;71(2):403-415. doi: 10.2144/btn-2021-0017. Epub 2021 Aug 05.

Due to the public health concerns of endocrine-disrupting chemicals, there is an increasing demand to develop improved high-throughput detection assays for enhanced exposure control and risk assessment. A substrate-free, autobioluminescent HEK293

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Xu T, Gilliam M, Sayler G, et al. Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells. Biotechniques. 2021;71(2):403-415doi: 10.2144/btn-2021-0017.
Xu, T., Gilliam, M., Sayler, G., Ripp, S., & Close, D. (2021). Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells. BioTechniques, 71(2), 403-415. https://doi.org/10.2144/btn-2021-0017
Xu, Tingting, et al. "Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells." BioTechniques vol. 71,2 (2021): 403-415. doi: https://doi.org/10.2144/btn-2021-0017
Xu T, Gilliam M, Sayler G, Ripp S, Close D. Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells. Biotechniques. 2021 Aug;71(2):403-415. doi: 10.2144/btn-2021-0017. Epub 2021 Aug 05. PMID: 34350768; PMCID: PMC8371548.
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Unbiased automated quantitation of ROS signals in live retinal neurons of .

BioTechniques

Deshpande P, Gogia N, Chimata AV, Singh A.
PMID: 34350780
Biotechniques. 2021 Aug;71(2):416-424. doi: 10.2144/btn-2021-0006. Epub 2021 Aug 05.

Numerous imaging modules are utilized to study changes that occur during cellular processes. Besides qualitative (immunohistochemical) or semiquantitative (Western blot) approaches, direct quantitation method(s) for detecting and analyzing signal intensities for disease(s) biomarkers are lacking. Thus, there is a...

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Deshpande P, Gogia N, Chimata AV, et al. Unbiased automated quantitation of ROS signals in live retinal neurons of . Biotechniques. 2021;71(2):416-424doi: 10.2144/btn-2021-0006.
Deshpande, P., Gogia, N., Chimata, A. V., & Singh, A. (2021). Unbiased automated quantitation of ROS signals in live retinal neurons of . BioTechniques, 71(2), 416-424. https://doi.org/10.2144/btn-2021-0006
Deshpande, Prajakta, et al. "Unbiased automated quantitation of ROS signals in live retinal neurons of ." BioTechniques vol. 71,2 (2021): 416-424. doi: https://doi.org/10.2144/btn-2021-0006
Deshpande P, Gogia N, Chimata AV, Singh A. Unbiased automated quantitation of ROS signals in live retinal neurons of . Biotechniques. 2021 Aug;71(2):416-424. doi: 10.2144/btn-2021-0006. Epub 2021 Aug 05. PMID: 34350780.
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Welcome to the 72nd Volume of .

BioTechniques

Torrington E, Free T, Sawyer A, Itano M.
PMID: 34846162
Biotechniques. 2022 Jan;72(1):1-4. doi: 10.2144/btn-2021-0112. Epub 2021 Nov 30.

No abstract available.

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Torrington E, Free T, Sawyer A, et al. Welcome to the 72nd Volume of . Biotechniques. 2021;72(1):1-4doi: 10.2144/btn-2021-0112.
Torrington, E., Free, T., Sawyer, A., & Itano, M. (2022). Welcome to the 72nd Volume of . BioTechniques, 72(1), 1-4. https://doi.org/10.2144/btn-2021-0112
Torrington, Ebony, et al. "Welcome to the 72nd Volume of ." BioTechniques vol. 72,1 (2022): 1-4. doi: https://doi.org/10.2144/btn-2021-0112
Torrington E, Free T, Sawyer A, Itano M. Welcome to the 72nd Volume of . Biotechniques. 2022 Jan;72(1):1-4. doi: 10.2144/btn-2021-0112. Epub 2021 Nov 30. PMID: 34846162.
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Strategies for increasing fertility in high productivity dairy herds.

Animal reproduction

Bragança LG, Zangirolamo AF.
PMID: 34457070
Anim Reprod. 2018 Aug 17;15(3):256-260. doi: 10.21451/1984-3143-AR2018-0079. eCollection 2018.

Over the years, the dairy cow has been selected and managed for high performance and efficiency in milk production. Thus, different factors influence the fertility of dairy cows of high productivity. It has been observed that genetic selection for...

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Bragança LG, Zangirolamo AF. Strategies for increasing fertility in high productivity dairy herds. Anim Reprod. 2018;15(3):256-260doi: 10.21451/1984-3143-AR2018-0079.
Bragança, L. G., & Zangirolamo, A. F. (2018). Strategies for increasing fertility in high productivity dairy herds. Animal reproduction, 15(3), 256-260. https://doi.org/10.21451/1984-3143-AR2018-0079
Bragança, Luiz Gustavo, and Zangirolamo, Amanda F. "Strategies for increasing fertility in high productivity dairy herds." Animal reproduction vol. 15,3 (2018): 256-260. doi: https://doi.org/10.21451/1984-3143-AR2018-0079
Bragança LG, Zangirolamo AF. Strategies for increasing fertility in high productivity dairy herds. Anim Reprod. 2018 Aug 17;15(3):256-260. doi: 10.21451/1984-3143-AR2018-0079. eCollection 2018. PMID: 34457070; PMCID: PMC8382311.
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Showing 193 to 203 of 203 entries