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Showing 2197 to 2206 of 2206 entries
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Revisiting mouse minute virus inactivation by high temperature short time treatment.

Biotechnology and bioengineering

Su J, Rice J, Hoffman J, Alvarado S, Bailey M, Kopp M, Murphy M, Kiss R, Barone PW, Wiebe ME, Springs SL, Dehghani H, Chen D.
PMID: 33913515
Biotechnol Bioeng. 2021 Aug;118(8):2967-2976. doi: 10.1002/bit.27805. Epub 2021 Jun 08.

In recent years, high temperature short time (HTST) treatment technology has been increasingly adopted for medium treatment to mitigate the potential risk of viral contamination in mammalian cell culture GMP manufacturing facilities. Mouse minute virus (MMV), also called minute...

Adropin decreases endothelial monolayer permeability after cell-free hemoglobin exposure and reduces MCP-1-induced macrophage transmigration.

Biochemical and biophysical research communications

Dodd WS, Patel D, Lucke-Wold B, Hosaka K, Chalouhi N, Hoh BL.
PMID: 34710824
Biochem Biophys Res Commun. 2021 Dec 10;582:105-110. doi: 10.1016/j.bbrc.2021.10.032. Epub 2021 Oct 16.

BACKGROUND: Cell-free heme-containing proteins mediate endothelial injury in a variety of disease states including subarachnoid hemorrhage and sepsis by increasing endothelial permeability. Inflammatory cells are also attracted to sites of vascular injury by monocyte chemotactic protein 1 (MCP-1) and...

Optimization of pre- treatments with Propidium Monoazide and PEMAX™ before real-time quantitative PCR for detection and quantification of viable Helicobacter pylori cells.

Journal of microbiological methods

Hortelano I, Moreno MY, García-Hernández J, Ferrús MA.
PMID: 33872638
J Microbiol Methods. 2021 Jun;185:106223. doi: 10.1016/j.mimet.2021.106223. Epub 2021 Apr 16.

Accurate detection of H. pylori in different environmental and clinical samples is essential for public health strtdudies. Now, a big effort is being made to design PCR methodologies that allow for the detection of viable and viable but non-culturable...

A New Role for Extracellular Vesicles in Cardiac Tissue Engineering and Regenerative Medicine.

Advanced nanobiomed research

Wagner KT, Radisic M.
PMID: 34927167
Adv Nanobiomed Res. 2021 Nov;1(11). doi: 10.1002/anbr.202100047. Epub 2021 Jun 24.

Cardiovascular diseases are the leading cause of death worldwide. Discovering new therapies to treat heart disease requires improved understanding of cardiac physiology at a cellular level. Extracellular vesicles (EVs) are plasma membrane-bound nano- and microparticles secreted by cells and...

High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling.

Stem cells translational medicine

Manstein F, Ullmann K, Kropp C, Halloin C, Triebert W, Franke A, Farr CM, Sahabian A, Haase A, Breitkreuz Y, Peitz M, Brüstle O, Kalies S, Martin U, Olmer R, Zweigerdt R.
PMID: 33660952
Stem Cells Transl Med. 2021 Jul;10(7):1063-1080. doi: 10.1002/sctm.20-0453. Epub 2021 Mar 04.

To harness the full potential of human pluripotent stem cells (hPSCs) we combined instrumented stirred tank bioreactor (STBR) technology with the power of in silico process modeling to overcome substantial, hPSC-specific hurdles toward their mass production. Perfused suspension culture...

Retinoprotective Effects Of Crocin And Crocetin via Anti-Angiogenic Mechanism in High Glucose-Induced Human Retinal Pigment Epithelium Cells.

Current molecular pharmacology

Sepahi S, Soheili ZS, Tavakkol-Afshari J, Mehri S, Hosseini SM, Mohajeri SA, Khodaverdi E.
PMID: 33881975
Curr Mol Pharmacol. 2021;14(5):883-893. doi: 10.2174/1874467214666210420111232.

BACKGROUND: Diabetic retinopathy (DR) is one of the most common side effects of diabetes. We aimed to investigate the effects of crocin and crocetin (as a deglycosylated form of crocin in blood stream) in gene expression or protein levels...

Measuring the density and viscosity of culture media for optimized computational fluid dynamics analysis of in vitro devices.

Journal of the mechanical behavior of biomedical materials

Poon C.
PMID: 34911025
J Mech Behav Biomed Mater. 2021 Dec 07;126:105024. doi: 10.1016/j.jmbbm.2021.105024. Epub 2021 Dec 07.

Culture medium is frequently modelled as water in computational fluid dynamics (CFD) analysis of in vitro culture systems involving flow, such as bioreactors and organ-on-chips. However, culture medium can be expected to have different properties to water due to...

Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model.

International journal of molecular sciences

Tremblay A, Simard M, Morin S, Pouliot R.
PMID: 34884896
Int J Mol Sci. 2021 Dec 03;22(23). doi: 10.3390/ijms222313091.

Healthy skin moLEdels produced by tissue-engineering often present a suboptimal skin barrier function as compared with normal human skin. Moreover, skin substitutes reconstructed according to the self-assembly method were found to be deficient in polyunsaturated fatty acids (PUFAs). Therefore,...

Chemical susceptibility testing of non-tuberculous mycobacterium strains and other aquatic bacteria: Results of a study for the development of a more sensitive and simple method for the detection of NTM in environmental samples.

Journal of microbiological methods

Ditommaso S, Giacomuzzi M, Memoli G, Garlasco J, Curtoni A, Iannaccone M, Zotti CM.
PMID: 34990646
J Microbiol Methods. 2022 Jan 03;193:106405. doi: 10.1016/j.mimet.2021.106405. Epub 2022 Jan 03.

The methods employed to detect non-tuberculous mycobacteria on environmental samples are essentially those classically used in clinical microbiology, which envisage a decontamination step to reduce the overgrowth of non-mycobacterial organisms before plating them on the culture medium. The aim...

Engineering novel designer glycopeptides as molecular carriers for boosting protein secretion in plant cell culture.

[No authors listed]
UIID-NSF: 430

1605564 Xu, Jianfeng With increasing needs for high-value protein-based therapeutics, plant cells offer a safe and cost-effective alternative bioproduction platform. However, low protein productivity is a common bottleneck in the commercialization of this technology. This project engineers plant cells...

Showing 2197 to 2206 of 2206 entries