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Showing 13 to 24 of 27 entries
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[Filamentous fungi as cellular factories: Biodiversity of secondary metabolites].

Revista iberoamericana de micologia

Gutiérrez S, Casqueiro J, Martín JF.
PMID: 15762783
Rev Iberoam Micol. 2000 Mar;17(1):S54-60.

The increase in the production of beta-lactam antibiotics has been carried out traditionally by classical mutagenic techniques, this method has been shown to be very effective and it has been the responsible for high increases in production. The development...

Persistence of HBV Vaccine's Protection and Response to Hepatitis B Booster Immunization in 5- to 7-Year-Old Children in the Kohgiloyeh and Boyerahmad Province, Iran.

Hepatitis monthly

Yazdanpanah B, Safari M, Yazdanpanah S.
PMID: 22308120
Hepat Mon. 2010;10(1):17-21. Epub 2010 Mar 01.

BACKGROUND AND AIMS: The duration of the protection of hepatitis B vaccination for infants and the necessity of a booster dose administration is unknown. The aim of the present study was to evaluate the persistence of seroprotection after hepatitis...

A novel recombinant baculovirus overexpressing a Bacillus thuringiensis Cry1Ab toxin enhances insecticidal activity.

Biological procedures online

El-Menofy W, Osman G, Assaeedi A, Salama M.
PMID: 24735532
Biol Proced Online. 2014 Apr 15;16:7. doi: 10.1186/1480-9222-16-7. eCollection 2014.

Baculoviruses have been genetically modified to express foreign genes under powerful promoters in order to accelerate their speed of killing. In this study a truncated form of cry1Ab gene derived from Bacillus thuringinsis (Bt) subsp. aegypti isolate Bt7 was...

Precision Tagging: A Novel Seamless Protein Tagging by Combinational Use of Type II and Type IIS Restriction Endonucleases.

Bio-protocol

Xu Z, Rui YN, Hagan JP, Kim DH.
PMID: 29770347
Bio Protoc. 2018 Feb 05;8(3). doi: 10.21769/BioProtoc.2721.

Protein tagging is a powerful tool for performing comprehensive analyses of the biological functions of a protein of interest owing to the existence of a wide variety of tags. It becomes indispensable in some cases, such as in tracking...

Nucleotide sequences of yeast genes for tRNA(2), tRNA(2) and tRNA(1): homology blocks occur in the vicinity of different tRNA genes.

The EMBO journal

Baker RE, Eigel A, Vögtel D, Feldmann H.
PMID: 16453416
EMBO J. 1982;1(3):291-5.

Three members of a collection of pBR322-yeast DNA recombinant plasmids containing yeast tRNA genes have been analyzed and sequenced. Each plasmid carries a single tRNA gene: pY44, tRNA(2); pY41, tRNA(2); pY7, tRNA(1). All three genes are intronless and terminate...

Construction and mapping of safflower chloroplast DNA recombinants and location of selected gene markers.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Ma C, Smith MA.
PMID: 24253119
Theor Appl Genet. 1985 Sep;70(6):620-7. doi: 10.1007/BF00252287.

DNA was isolated and purified from chloroplasts of safflower (Carthamus tinctorius L.), digested with HindIII restriction endonuclease, and ligated into the HindIII site of the plasmid pUC9. Recombinant DNAs were isolated from ampicillin resistant white colonies which grew in...

Insulin discovery: A pivotal point in medical history.

Metabolism: clinical and experimental

Falcetta P, Aragona M, Bertolotto A, Bianchi C, Campi F, Garofolo M, Del Prato S.
PMID: 34838778
Metabolism. 2021 Nov 25;127:154941. doi: 10.1016/j.metabol.2021.154941. Epub 2021 Nov 25.

The discovery of insulin in 1921 - due to the efforts of the Canadian research team based in Toronto - has been a landmark achievement in the history of medicine. Lives of people with diabetes were changed forever, considering...

A Descriptive-Multivariate Analysis of Community Knowledge, Confidence, and Trust in COVID-19 Clinical Trials among Healthcare Workers in Uganda.

Vaccines

Kasozi KI, Laudisoit A, Osuwat LO, Batiha GE, Al Omairi NE, Aigbogun E, Ninsiima HI, Usman IM, DeTora LM, MacLeod ET, Nalugo H, Crawley FP, Bierer BE, Mwandah DC, Kato CD, Kiyimba K, Ayikobua ET, Lillian L, Matama K, Nelly Mak SC, Onanyang D, Pius T, Nalumenya DP, Ssebuufu R, Rugambwa NO, Musoke GH, Bardosh K, Ochieng JJ, Ssempijja F, Kyamanywa P, Tumwine G, Alzahrani KJ, Welburn SC.
PMID: 33809269
Vaccines (Basel). 2021 Mar 12;9(3). doi: 10.3390/vaccines9030253.

Background-misinformation and mistrust often undermines community vaccine uptake, yet information in rural communities, especially of developing countries, is scarce. This study aimed to identify major challenges associated with coronavirus disease 2019 (COVID-19) vaccine clinical trials among healthcare workers and...

Human Growth and Growth Hormone: From Antiquity to the Recominant Age to the Future.

Frontiers in endocrinology

Graber E, Reiter EO, Rogol AD.
PMID: 34290673
Front Endocrinol (Lausanne). 2021 Jul 05;12:709936. doi: 10.3389/fendo.2021.709936. eCollection 2021.

Since antiquity Man has been fascinated by the variations in human (and animal) growth. Stories and art abound about giants and little people. Modern genetics have solved some of etiologies at both extremes of growth. Serious study began with...

Expression and fate of CAT reporter gene microinjected into fertilized medaka (Oryzias latipes) eggs in the form of plasmid DNA, recombinant phage particles and its DNA.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Chong SS, Vielkind JR.
PMID: 24227244
Theor Appl Genet. 1989 Sep;78(3):369-80. doi: 10.1007/BF00265299.

Fertilized medaka (Oryzias latipes) eggs were cytoplasmically injected with the chloramphenicol acetyltransferase (CAT) gene encompassed in supercoiled and linear plasmid DNA, as well as in intact recombinant phage particles and DNA isolated from the phage. Expression for the CAT...

Targeted treatment of liver metastasis from gastric cancer using specific binding peptide.

American journal of translational research

Gong J, Tan G, Sheng N, You W, Wang Z.
PMID: 27347305
Am J Transl Res. 2016 May 15;8(5):1945-56. eCollection 2016.

Gastric cancer ranks the first in China among all gastrointestinal cancers in terms of incidence, and liver metastasis is the leading cause of death for patients with advanced gastric cancer. Tumor necrosis factor (TNF) is a cytokine commonly chosen...

Human bone morphogenetic protein-2 (hBMP-2) characterization by physical-chemical, immunological and biological assays.

AMB Express

Suzuki MF, Oliveira JE, Damiani R, Lima ER, Amaral KC, Santos AMS, Magalhães GS, Faverani LP, Pereira LAVD, Silva FM, Bartolini P.
PMID: 32067115
AMB Express. 2020 Feb 17;10(1):34. doi: 10.1186/s13568-020-0964-5.

Commercially available preparations of methionyl-human BMP-2 and CHO-derived hBMP-2, which belongs to the transforming growth factor β (TGF-β) superfamily, were used for a complete characterization. This protein is an extremely efficient osteoinductor that plays an important role during bone...

Showing 13 to 24 of 27 entries