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Showing 13 to 24 of 885 entries
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Multiscattering-enhanced optical biosensor: multiplexed, non-invasive and continuous measurements of cellular processes.

Biomedical optics express

Koman VB, Santschi C, Martin OJ.
PMID: 26203366
Biomed Opt Express. 2015 Jun 05;6(7):2353-65. doi: 10.1364/BOE.6.002353. eCollection 2015 Jul 01.

The continuous measurement of uptake or release of biomarkers provides invaluable information for understanding and monitoring the metabolism of cells. In this work, a multiscattering-enhanced optical biosensor for the multiplexed, non-invasive, and continuous detection of hydrogen peroxide (H2O2), lactate...

Intestinal glucose uptake responses to infusion of glucose, fructose and galactose in dogs Intestinal glucose uptake responses to infusion of glucose, fructose and galactose in dogs.

Nigerian journal of physiological sciences : official publication of the Physiological Society of Nigeria

Salman TM, Alada AR, Oyebola DD.
PMID: 26196562
Niger J Physiol Sci. 2014 Jun 19;29(1):23-7.

The present study was designed to investigate the effects of intravanous (i.v) infusion of fructose, galactose and glucose on canine IGU during postprandial state. Experiments were carried out on fasted, male, anaesthethized adult mongrel dogs divided into four groups...

Tissue printing for myrosinase activity in roots of turnip and Japanese radish and horseradish: a technique for localizing myrosinases.

Plant science : an international journal of experimental plant biology

Hara M, Eto H, Kuboi T.
PMID: 11166428
Plant Sci. 2001 Feb 05;160(3):425-431. doi: 10.1016/s0168-9452(00)00400-3.

A method for analyzing the tissue distribution of myrosinase activity in Brassicaceous plants was developed. This technique is based on 'tissue printing' to visualize enzyme activity. The freshly-cut surface (transverse direction) of the root of three species, Japanese radish...

Salivary glucose oxidase: multifunctional roles for helicoverpa zea?.

Archives of insect biochemistry and physiology

Eichenseer H, Mathews MC, Bi JL, Murphy JB, Felton GW.
PMID: 10467060
Arch Insect Biochem Physiol. 1999 Sep;42(1):99-109. doi: 10.1002/(SICI)1520-6327(199909)42:1<99::AID-ARCH10>3.0.CO;2-B.

Labial glands of Helicoverpa zea produced a glucose oxidase (GOX), which was present in the saliva and midgut lumen. We purified GOX 23-fold by isoelectric focusing of labial gland homogenates and investigated physical and kinetic properties of the enzyme....

Microscopic measurement of pH with iridium oxide microelectrodes.

Analytical chemistry

Wipf DO, Ge F, Spaine TW, Baur JE.
PMID: 11055710
Anal Chem. 2000 Oct 15;72(20):4921-7. doi: 10.1021/ac000383j.

Microscopic pH electrodes were produced by deposition of hydrous iridium oxide onto carbon fiber microelectrodes. The electrodes exhibit two linear regions of potentiometric response between pH 2-6 and pH 6-12. The electrodes respond to pH changes within 50 ms,...

The Study of a New Type of Membrane for Biosensor.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Wang SG, Ji XS, Yuan ZY.
PMID: 12232640
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(4):442-446.

Polyvinyl alcohol (PVA) was selected to develop a new type of membrane for biosensor. The PVA membrane was first activated by cyanogens bromide, followed by coupling with ethyl diamine. After treated by glutaraldehyde, glucose oxidase and lactate oxidase were...

Enzyme modified microband electrodes: cross-talk effects and their elimination.

The Analyst

Quinto M, Koudelka-Hep M, Palmisano F.
PMID: 11478637
Analyst. 2001 Jul;126(7):1068-72. doi: 10.1039/b101529j.

One microband of an array of four microband electrodes (1 mm long and 25 microm wide with a 25 microm gap) was modified with glucose oxidase by direct electrochemically assisted immobilisation, giving a stable microbiosensor with an apparent Michaelis-Menten...

Effect of interfering substances on current response of recombinant peroxidase and glucose oxidase-recombinant peroxidase modified graphite electrodes.

The Analyst

Dock E, Lindgren A, Ruzgas T, Gorton L.
PMID: 11763069
Analyst. 2001 Nov;126(11):1929-35. doi: 10.1039/b104815p.

Graphite electrodes have been modified with different forms of horseradish peroxidase (HRP). These included native HRP, wild-type recombinant HRP, and two single-point recombinant HRP mutants, N70V and N70D. The mediator-less response of these electrodes to H2O2 was studied indicating...

Detection of pepsin and glucose in tracheal secretions as indicators of aspiration in mechanically ventilated children.

Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies

Meert KL, Daphtary KM, Metheny NA.
PMID: 12793917
Pediatr Crit Care Med. 2002 Jan;3(1):19-22. doi: 10.1097/00130478-200201000-00005.

OBJECTIVES: The detection of glucose in tracheal secretions has been used as an indicator of aspiration in mechanically ventilated, tube-fed children. Pepsin detection may be a more specific indicator. We determined the frequency of pepsin and glucose detection in...

Multilayered assembly of dendrimers with enzymes on gold: thickness-controlled biosensing interface.

Analytical chemistry

Yoon HC, Kim HS.
PMID: 10739193
Anal Chem. 2000 Mar 01;72(5):922-6. doi: 10.1021/ac991299a.

A new approach to construct a multilayered enzyme film on the Au surface for use as a biosensing interface is described. The film was prepared by alternate layer-by-layer depositions of G4 poly(amidoamine) dendrimers and periodate-oxidized glucose oxidase (GOx). The...

Effects of stress on exacerbation of diabetes mellitus, serum glucose and cortisol levels and body weight in rats.

Pathophysiology : the official journal of the International Society for Pathophysiology

Radahmadi M, Shadan F, Karimian SM, Sadr SS, Nasimi A.
PMID: 16102950
Pathophysiology. 2006 Feb 21;13(1):51-5. doi: 10.1016/j.pathophys.2005.07.001. Epub 2005 Aug 15.

OBJECTIVE: The effects of stress on the serum glucose, serum cortisol levels and body weight were investigated to clarify the possible link between the stress and diabetes.METHODS: The experiments were performed on nondiabetic and streptozotocin diabetic rats divided to...

Fluorometric sensors based on chemically modified enzymes Glucose determination in drinks.

Talanta

Sanz V, Galban J, de Marcos S, Castillo JR.
PMID: 18969063
Talanta. 2003 Jun 13;60(2):415-23. doi: 10.1016/S0039-9140(03)00075-4.

In this paper an enzymatic fluorometric sensor for glucose determination in drinks is presented. The sensor film was obtained by immobilisation of glucose oxidase chemically modified with a fluorescein derivative (GOx-FS) in a polyacrylamide polymer. During the enzymatic reaction...

Showing 13 to 24 of 885 entries