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Dynamic Stabilization of Unit Polyion Complexes Incorporating Small Interfering RNA by Fine-Tuning of Cationic Block Length in Two-Branched Poly(ethylene glycol)-.

Biomacromolecules

Chaya H, Naito M, Cho M, Toh K, Hayashi K, Fukushima S, Yamasaki Y, Kataoka K, Miyata K.
PMID: 34935361
Biomacromolecules. 2022 Jan 10;23(1):388-397. doi: 10.1021/acs.biomac.1c01344. Epub 2021 Dec 22.

To stabilize small interfering RNA (siRNA) in the bloodstream for systemic RNAi therapeutics, we previously fabricated ultrasmall siRNA nanocarriers that were sub-20 nm in hydrodynamic diameter, named as unit polyion complexes (uPICs), using two-branched poly(ethylene glycol)-

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Chaya H, Naito M, Cho M, et al. Dynamic Stabilization of Unit Polyion Complexes Incorporating Small Interfering RNA by Fine-Tuning of Cationic Block Length in Two-Branched Poly(ethylene glycol)-. Biomacromolecules. 2021;23(1):388-397doi: 10.1021/acs.biomac.1c01344.
Chaya, H., Naito, M., Cho, M., Toh, K., Hayashi, K., Fukushima, S., Yamasaki, Y., Kataoka, K., & Miyata, K. (2022). Dynamic Stabilization of Unit Polyion Complexes Incorporating Small Interfering RNA by Fine-Tuning of Cationic Block Length in Two-Branched Poly(ethylene glycol)-. Biomacromolecules, 23(1), 388-397. https://doi.org/10.1021/acs.biomac.1c01344
Chaya, Hiroyuki, et al. "Dynamic Stabilization of Unit Polyion Complexes Incorporating Small Interfering RNA by Fine-Tuning of Cationic Block Length in Two-Branched Poly(ethylene glycol)-." Biomacromolecules vol. 23,1 (2022): 388-397. doi: https://doi.org/10.1021/acs.biomac.1c01344
Chaya H, Naito M, Cho M, Toh K, Hayashi K, Fukushima S, Yamasaki Y, Kataoka K, Miyata K. Dynamic Stabilization of Unit Polyion Complexes Incorporating Small Interfering RNA by Fine-Tuning of Cationic Block Length in Two-Branched Poly(ethylene glycol)-. Biomacromolecules. 2022 Jan 10;23(1):388-397. doi: 10.1021/acs.biomac.1c01344. Epub 2021 Dec 22. PMID: 34935361.
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Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift.

bioRxiv : the preprint server for biology

Cameroni E, Saliba C, Bowen JE, Rosen LE, Culap K, Pinto D, VanBlargan LA, De Marco A, Zepeda SK, Iulio JD, Zatta F, Kaiser H, Noack J, Farhat N, Czudnochowski N, Havenar-Daughton C, Sprouse KR, Dillen JR, Powell AE, Chen A, Maher C, Yin L, Sun D, Soriaga L, Bassi J, Silacci-Fregni C, Gustafsson C, Franko NM, Logue J, Iqbal NT, Mazzitelli I, Geffner J, Grifantini R, Chu H, Gori A, Riva A, Giannini O, Ceschi A, Ferrari P, Cippà P, Franzetti-Pellanda A, Garzoni C, Halfmann PJ, Kawaoka Y, Hebner C, Purcell LA, Piccoli L, Pizzuto MS, Walls AC, Diamond MS, Telenti A, Virgin HW, Lanzavecchia A, Veesler D, Snell G, Corti D.
PMID: 34931194
bioRxiv. 2021 Dec 20; doi: 10.1101/2021.12.12.472269.

The recently emerged SARS-CoV-2 Omicron variant harbors 37 amino acid substitutions in the spike (S) protein, 15 of which are in the receptor-binding domain (RBD), thereby raising concerns about the effectiveness of available vaccines and antibody therapeutics. Here, we...

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Cameroni E, Saliba C, Bowen JE, et al. Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift. bioRxiv. 2021;doi: 10.1101/2021.12.12.472269.
Cameroni, E., Saliba, C., Bowen, J. E., Rosen, L. E., Culap, K., Pinto, D., VanBlargan, L. A., De Marco, A., Zepeda, S. K., Iulio, J. D., Zatta, F., Kaiser, H., Noack, J., Farhat, N., Czudnochowski, N., Havenar-Daughton, C., Sprouse, K. R., Dillen, J. R., Powell, A. E., Chen, A., Maher, C., Yin, L., Sun, D., Soriaga, L., Bassi, J., Silacci-Fregni, C., Gustafsson, C., Franko, N. M., Logue, J., Iqbal, N. T., Mazzitelli, I., Geffner, J., Grifantini, R., Chu, H., Gori, A., Riva, A., Giannini, O., Ceschi, A., Ferrari, P., Cippà, P., Franzetti-Pellanda, A., Garzoni, C., Halfmann, P. J., Kawaoka, Y., Hebner, C., Purcell, L. A., Piccoli, L., Pizzuto, M. S., Walls, A. C., Diamond, M. S., Telenti, A., Virgin, H. W., Lanzavecchia, A., Veesler, D., Snell, G., & Corti, D. (2021). Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift. bioRxiv : the preprint server for biology, . https://doi.org/10.1101/2021.12.12.472269
Cameroni, Elisabetta, et al. "Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift." bioRxiv : the preprint server for biology vol. (2021). doi: https://doi.org/10.1101/2021.12.12.472269
Cameroni E, Saliba C, Bowen JE, Rosen LE, Culap K, Pinto D, VanBlargan LA, De Marco A, Zepeda SK, Iulio JD, Zatta F, Kaiser H, Noack J, Farhat N, Czudnochowski N, Havenar-Daughton C, Sprouse KR, Dillen JR, Powell AE, Chen A, Maher C, Yin L, Sun D, Soriaga L, Bassi J, Silacci-Fregni C, Gustafsson C, Franko NM, Logue J, Iqbal NT, Mazzitelli I, Geffner J, Grifantini R, Chu H, Gori A, Riva A, Giannini O, Ceschi A, Ferrari P, Cippà P, Franzetti-Pellanda A, Garzoni C, Halfmann PJ, Kawaoka Y, Hebner C, Purcell LA, Piccoli L, Pizzuto MS, Walls AC, Diamond MS, Telenti A, Virgin HW, Lanzavecchia A, Veesler D, Snell G, Corti D. Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift. bioRxiv. 2021 Dec 20; doi: 10.1101/2021.12.12.472269. PMID: 34931194; PMCID: PMC8687478.
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Inconvenience and adaptation in Japanese adult achondroplasia and hypochondroplasia: A cross-sectional study.

Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology

Ajimi A, Matsushita M, Mishima K, Haga N, Fujiwara S, Ozono K, Kubota T, Kitaoka T, Imagama S, Kitoh H.
PMID: 35002064
Clin Pediatr Endocrinol. 2022;31(1):18-24. doi: 10.1297/cpe.2021-0043. Epub 2021 Nov 01.

The health-related quality of life is reduced in patients with achondroplasia (ACH) and hypochondroplasia (HCH); however, the detailed inconveniences in the daily living and individual adaptations have not been elucidated. This study aimed to evaluate the inconvenience and adaptation...

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Ajimi A, Matsushita M, Mishima K, et al. Inconvenience and adaptation in Japanese adult achondroplasia and hypochondroplasia: A cross-sectional study. Clin Pediatr Endocrinol. 2021;31(1):18-24doi: 10.1297/cpe.2021-0043.
Ajimi, A., Matsushita, M., Mishima, K., Haga, N., Fujiwara, S., Ozono, K., Kubota, T., Kitaoka, T., Imagama, S., & Kitoh, H. (2022). Inconvenience and adaptation in Japanese adult achondroplasia and hypochondroplasia: A cross-sectional study. Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology, 31(1), 18-24. https://doi.org/10.1297/cpe.2021-0043
Ajimi, Akiko, et al. "Inconvenience and adaptation in Japanese adult achondroplasia and hypochondroplasia: A cross-sectional study." Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology vol. 31,1 (2022): 18-24. doi: https://doi.org/10.1297/cpe.2021-0043
Ajimi A, Matsushita M, Mishima K, Haga N, Fujiwara S, Ozono K, Kubota T, Kitaoka T, Imagama S, Kitoh H. Inconvenience and adaptation in Japanese adult achondroplasia and hypochondroplasia: A cross-sectional study. Clin Pediatr Endocrinol. 2022;31(1):18-24. doi: 10.1297/cpe.2021-0043. Epub 2021 Nov 01. PMID: 35002064; PMCID: PMC8713058.
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Dual kinetics of OATP2B1: Inhibitory potency and pH-dependence of OATP2B1 inhibitors.

Drug metabolism and pharmacokinetics

Sato R, Akiyoshi T, Morita T, Katayama K, Yajima K, Kataoka H, Imaoka A, Ohtani H.
PMID: 34619547
Drug Metab Pharmacokinet. 2021 Dec;41:100416. doi: 10.1016/j.dmpk.2021.100416. Epub 2021 Aug 04.

Organic anion transporting polypeptide (OATP) 2B1 is expressed in the intestine and liver, and OATP2B1-mediated transport of estrone 3-sulfate is pH-dependent and consists of: the high-affinity component (Hc) and low-affinity component (Lc). This study aimed to evaluate the influence...

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Sato R, Akiyoshi T, Morita T, et al. Dual kinetics of OATP2B1: Inhibitory potency and pH-dependence of OATP2B1 inhibitors. Drug Metab Pharmacokinet. 2021;41:100416doi: 10.1016/j.dmpk.2021.100416.
Sato, R., Akiyoshi, T., Morita, T., Katayama, K., Yajima, K., Kataoka, H., Imaoka, A., & Ohtani, H. (2021). Dual kinetics of OATP2B1: Inhibitory potency and pH-dependence of OATP2B1 inhibitors. Drug metabolism and pharmacokinetics, 41100416. https://doi.org/10.1016/j.dmpk.2021.100416
Sato, Ryo, et al. "Dual kinetics of OATP2B1: Inhibitory potency and pH-dependence of OATP2B1 inhibitors." Drug metabolism and pharmacokinetics vol. 41 (2021): 100416. doi: https://doi.org/10.1016/j.dmpk.2021.100416
Sato R, Akiyoshi T, Morita T, Katayama K, Yajima K, Kataoka H, Imaoka A, Ohtani H. Dual kinetics of OATP2B1: Inhibitory potency and pH-dependence of OATP2B1 inhibitors. Drug Metab Pharmacokinet. 2021 Dec;41:100416. doi: 10.1016/j.dmpk.2021.100416. Epub 2021 Aug 04. PMID: 34619547.
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Risk Assessment of Passive Smoking Based on Analysis of Hair Nicotine and Cotinine as Exposure Biomarkers by In-Tube Solid-Phase Microextraction Coupled On-Line to LC-MS/MS.

Molecules (Basel, Switzerland)

Kataoka H, Kaji S, Moai M.
PMID: 34885941
Molecules. 2021 Dec 03;26(23). doi: 10.3390/molecules26237356.

Passive smoking due to environmental tobacco smoke is a serious public health concern because it increases the risk of lung cancer and cardiovascular disease. However, the current status and effect of passive smoking in various lifestyles are not fully...

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Kataoka H, Kaji S, Moai M. Risk Assessment of Passive Smoking Based on Analysis of Hair Nicotine and Cotinine as Exposure Biomarkers by In-Tube Solid-Phase Microextraction Coupled On-Line to LC-MS/MS. Molecules. 2021;26(23)doi: 10.3390/molecules26237356.
Kataoka, H., Kaji, S., & Moai, M. (2021). Risk Assessment of Passive Smoking Based on Analysis of Hair Nicotine and Cotinine as Exposure Biomarkers by In-Tube Solid-Phase Microextraction Coupled On-Line to LC-MS/MS. Molecules (Basel, Switzerland), 26(23), . https://doi.org/10.3390/molecules26237356
Kataoka, Hiroyuki, et al. "Risk Assessment of Passive Smoking Based on Analysis of Hair Nicotine and Cotinine as Exposure Biomarkers by In-Tube Solid-Phase Microextraction Coupled On-Line to LC-MS/MS." Molecules (Basel, Switzerland) vol. 26,23 (2021). doi: https://doi.org/10.3390/molecules26237356
Kataoka H, Kaji S, Moai M. Risk Assessment of Passive Smoking Based on Analysis of Hair Nicotine and Cotinine as Exposure Biomarkers by In-Tube Solid-Phase Microextraction Coupled On-Line to LC-MS/MS. Molecules. 2021 Dec 03;26(23). doi: 10.3390/molecules26237356. PMID: 34885941; PMCID: PMC8659248.
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Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation.

Nature

Saito A, Irie T, Suzuki R, Maemura T, Nasser H, Uriu K, Kosugi Y, Shirakawa K, Sadamasu K, Kimura I, Ito J, Wu J, Iwatsuki-Horimoto K, Ito M, Yamayoshi S, Loeber S, Tsuda M, Wang L, Ozono S, Butlertanaka EP, Tanaka YL, Shimizu R, Shimizu K, Yoshimatsu K, Kawabata R, Sakaguchi T, Tokunaga K, Yoshida I, Asakura H, Nagashima M, Kazuma Y, Nomura R, Horisawa Y, Yoshimura K, Takaori-Kondo A, Imai M, Tanaka S, Nakagawa S, Ikeda T, Fukuhara T, Kawaoka Y, Sato K.
PMID: 34823256
Nature. 2021 Nov 25; doi: 10.1038/s41586-021-04266-9. Epub 2021 Nov 25.

During the current coronavirus disease 2019 (COVID-19) pandemic, a variety of mutations have accumulated in the viral genome of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and, at the time of writing, four variants of concern are considered to...

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Saito A, Irie T, Suzuki R, et al. Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation. Nature. 2021;doi: 10.1038/s41586-021-04266-9.
Saito, A., Irie, T., Suzuki, R., Maemura, T., Nasser, H., Uriu, K., Kosugi, Y., Shirakawa, K., Sadamasu, K., Kimura, I., Ito, J., Wu, J., Iwatsuki-Horimoto, K., Ito, M., Yamayoshi, S., Loeber, S., Tsuda, M., Wang, L., Ozono, S., Butlertanaka, E. P., Tanaka, Y. L., Shimizu, R., Shimizu, K., Yoshimatsu, K., Kawabata, R., Sakaguchi, T., Tokunaga, K., Yoshida, I., Asakura, H., Nagashima, M., Kazuma, Y., Nomura, R., Horisawa, Y., Yoshimura, K., Takaori-Kondo, A., Imai, M., Tanaka, S., Nakagawa, S., Ikeda, T., Fukuhara, T., Kawaoka, Y., & Sato, K. (2021). Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation. Nature, . https://doi.org/10.1038/s41586-021-04266-9
Saito, Akatsuki, et al. "Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation." Nature vol. (2021). doi: https://doi.org/10.1038/s41586-021-04266-9
Saito A, Irie T, Suzuki R, Maemura T, Nasser H, Uriu K, Kosugi Y, Shirakawa K, Sadamasu K, Kimura I, Ito J, Wu J, Iwatsuki-Horimoto K, Ito M, Yamayoshi S, Loeber S, Tsuda M, Wang L, Ozono S, Butlertanaka EP, Tanaka YL, Shimizu R, Shimizu K, Yoshimatsu K, Kawabata R, Sakaguchi T, Tokunaga K, Yoshida I, Asakura H, Nagashima M, Kazuma Y, Nomura R, Horisawa Y, Yoshimura K, Takaori-Kondo A, Imai M, Tanaka S, Nakagawa S, Ikeda T, Fukuhara T, Kawaoka Y, Sato K. Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation. Nature. 2021 Nov 25; doi: 10.1038/s41586-021-04266-9. Epub 2021 Nov 25. PMID: 34823256.
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Reconfirmation of the accuracy of the taller-than-wide sign in multicenter collaborative research in Japan.

Endocrine journal

Fukushima M, Fukunari N, Murakami T, Kunii Y, Suzuki S, Kitaoka M.
PMID: 33790086
Endocr J. 2021 Aug 28;68(8):897-904. doi: 10.1507/endocrj.EJ20-0379. Epub 2021 Apr 01.

The taller-than-wide sign indicates that the anteroposterior dimension-to-transverse dimension ratio (AP/T ratio) is higher than 1. The aim of the present study was to reconfirm the accuracy of the taller-than-wide sign for diagnosing malignant thyroid nodules by ultrasonography in...

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Fukushima M, Fukunari N, Murakami T, et al. Reconfirmation of the accuracy of the taller-than-wide sign in multicenter collaborative research in Japan. Endocr J. 2021;68(8):897-904doi: 10.1507/endocrj.EJ20-0379.
Fukushima, M., Fukunari, N., Murakami, T., Kunii, Y., Suzuki, S., & Kitaoka, M. (2021). Reconfirmation of the accuracy of the taller-than-wide sign in multicenter collaborative research in Japan. Endocrine journal, 68(8), 897-904. https://doi.org/10.1507/endocrj.EJ20-0379
Fukushima, Mitsuhiro, et al. "Reconfirmation of the accuracy of the taller-than-wide sign in multicenter collaborative research in Japan." Endocrine journal vol. 68,8 (2021): 897-904. doi: https://doi.org/10.1507/endocrj.EJ20-0379
Fukushima M, Fukunari N, Murakami T, Kunii Y, Suzuki S, Kitaoka M. Reconfirmation of the accuracy of the taller-than-wide sign in multicenter collaborative research in Japan. Endocr J. 2021 Aug 28;68(8):897-904. doi: 10.1507/endocrj.EJ20-0379. Epub 2021 Apr 01. PMID: 33790086.
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Health-Related Quality of Life May Predict Disease Staging in 10 Years in Parkinson Disease With Hoehn-Yahr Stage III.

Neurology. Clinical practice

Kataoka H, Sugie K.
PMID: 34484900
Neurol Clin Pract. 2021 Jun;11(3):e261-e266. doi: 10.1212/CPJ.0000000000000924.

BACKGROUND: Ten years ago, we examined 30 patients with Hoehn-Yahr stage III Parkinson disease (PD) to determine predictors related to falling, including walking speed, step number, and step time by using the originally designed narrowed paths. We verified the...

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Kataoka H, Sugie K. Health-Related Quality of Life May Predict Disease Staging in 10 Years in Parkinson Disease With Hoehn-Yahr Stage III. Neurol Clin Pract. 2021;11(3):e261-e266doi: 10.1212/CPJ.0000000000000924.
Kataoka, H., & Sugie, K. (2021). Health-Related Quality of Life May Predict Disease Staging in 10 Years in Parkinson Disease With Hoehn-Yahr Stage III. Neurology. Clinical practice, 11(3), e261-e266. https://doi.org/10.1212/CPJ.0000000000000924
Kataoka, Hiroshi, and Sugie, Kazuma. "Health-Related Quality of Life May Predict Disease Staging in 10 Years in Parkinson Disease With Hoehn-Yahr Stage III." Neurology. Clinical practice vol. 11,3 (2021): e261-e266. doi: https://doi.org/10.1212/CPJ.0000000000000924
Kataoka H, Sugie K. Health-Related Quality of Life May Predict Disease Staging in 10 Years in Parkinson Disease With Hoehn-Yahr Stage III. Neurol Clin Pract. 2021 Jun;11(3):e261-e266. doi: 10.1212/CPJ.0000000000000924. PMID: 34484900; PMCID: PMC8382375.
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Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift.

Nature

Cameroni E, Bowen JE, Rosen LE, Saliba C, Zepeda SK, Culap K, Pinto D, VanBlargan LA, De Marco A, di Iulio J, Zatta F, Kaiser H, Noack J, Farhat N, Czudnochowski N, Havenar-Daughton C, Sprouse KR, Dillen JR, Powell AE, Chen A, Maher C, Yin L, Sun D, Soriaga L, Bassi J, Silacci-Fregni C, Gustafsson C, Franko NM, Logue J, Iqbal NT, Mazzitelli I, Geffner J, Grifantini R, Chu H, Gori A, Riva A, Giannini O, Ceschi A, Ferrari P, Cippà PE, Franzetti-Pellanda A, Garzoni C, Halfmann PJ, Kawaoka Y, Hebner C, Purcell LA, Piccoli L, Pizzuto MS, Walls AC, Diamond MS, Telenti A, Virgin HW, Lanzavecchia A, Snell G, Veesler D, Corti D.
PMID: 35016195
Nature. 2021 Dec 23; doi: 10.1038/s41586-021-04386-2. Epub 2021 Dec 23.

The recently emerged SARS-CoV-2 Omicron variant encodes 37 amino acid substitutions in the spike (S) protein, 15 of which are in the receptor-binding domain (RBD), thereby raising concerns about the effectiveness of available vaccines and antibody therapeutics. Here, we...

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Cameroni E, Bowen JE, Rosen LE, et al. Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift. Nature. 2021;doi: 10.1038/s41586-021-04386-2.
Cameroni, E., Bowen, J. E., Rosen, L. E., Saliba, C., Zepeda, S. K., Culap, K., Pinto, D., VanBlargan, L. A., De Marco, A., di Iulio, J., Zatta, F., Kaiser, H., Noack, J., Farhat, N., Czudnochowski, N., Havenar-Daughton, C., Sprouse, K. R., Dillen, J. R., Powell, A. E., Chen, A., Maher, C., Yin, L., Sun, D., Soriaga, L., Bassi, J., Silacci-Fregni, C., Gustafsson, C., Franko, N. M., Logue, J., Iqbal, N. T., Mazzitelli, I., Geffner, J., Grifantini, R., Chu, H., Gori, A., Riva, A., Giannini, O., Ceschi, A., Ferrari, P., Cippà, P. E., Franzetti-Pellanda, A., Garzoni, C., Halfmann, P. J., Kawaoka, Y., Hebner, C., Purcell, L. A., Piccoli, L., Pizzuto, M. S., Walls, A. C., Diamond, M. S., Telenti, A., Virgin, H. W., Lanzavecchia, A., Snell, G., Veesler, D., & Corti, D. (2021). Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift. Nature, . https://doi.org/10.1038/s41586-021-04386-2
Cameroni, Elisabetta, et al. "Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift." Nature vol. (2021). doi: https://doi.org/10.1038/s41586-021-04386-2
Cameroni E, Bowen JE, Rosen LE, Saliba C, Zepeda SK, Culap K, Pinto D, VanBlargan LA, De Marco A, di Iulio J, Zatta F, Kaiser H, Noack J, Farhat N, Czudnochowski N, Havenar-Daughton C, Sprouse KR, Dillen JR, Powell AE, Chen A, Maher C, Yin L, Sun D, Soriaga L, Bassi J, Silacci-Fregni C, Gustafsson C, Franko NM, Logue J, Iqbal NT, Mazzitelli I, Geffner J, Grifantini R, Chu H, Gori A, Riva A, Giannini O, Ceschi A, Ferrari P, Cippà PE, Franzetti-Pellanda A, Garzoni C, Halfmann PJ, Kawaoka Y, Hebner C, Purcell LA, Piccoli L, Pizzuto MS, Walls AC, Diamond MS, Telenti A, Virgin HW, Lanzavecchia A, Snell G, Veesler D, Corti D. Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift. Nature. 2021 Dec 23; doi: 10.1038/s41586-021-04386-2. Epub 2021 Dec 23. PMID: 35016195.
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